Simultaneous Determination of Mebeverine Hydrochloride and Sulpiride Using the First Derivatives of Ratio Spectra and Chemometric Methods

Abstract

Mebeverine hydrochloride (MB), 4-[ethyl(4-methoxy-α-methylphenethyl)amino]butyl veratrate hydrochloride [2753-45-9], is a potent direct antispasmodic acting mainly on the smooth muscles of the gastrointestinal tract; it is particularly effective against colonic spasms. 1 Sulpiride (SU), N-(1-ethylpyrrolidin-2-ylmethyl)-2-methoxy-5-sulfamoyl benzamide [15676-16-1], is a substituted benzamide antipsychotic which is reported to be a selective antagonist of central dopamine (D2, D3 and D4) receptors. It is also claimed to have mood-elevating properties. 1The binary mixture of the two drugs is used in the treatment of gastointestinal and colic spasms, specially in the presence of psychasomatic manifestations of nervous tension, mental stress or anxiety. Several analytical methods have been used for the determination of MB, including high performance liquid chromatography (HPLC), 2-4 thin layer chromatography (TLC), 5,6 spectrophotometric methods 25 first used derivative UV spectrophotometry, TLC-densitometry and HPLC for the simultaneous determination of both of these two compounds in pharmaceutical tablets. In this study, ratio spectra first derivative spectrophotometry and classical least squares method are proposed for simultaneous determination of MB and SU in their mixtures and their pharmaceutical preparation, Colona tablets. The proposed ratio spectra first derivative method was found to be more sensitive than the published UV first derivative method 25 (Beer's law linear range was 4.0 -40.0 and 1.0 -10.0 µg/ml for MB and SU, respectively while the linear range was 10.0 -30.0 and 2.0 -8.0 µg/ml in the first derivative published method). The proposed chemometric method was more rapid, simple than the published methods. 25 Also, the proposed methods can be used as alternative methods to those reported for the simultaneous determination of MB and SU. Experimental ApparatusA Shimadzu 1601 double beam UV-Vis spectrophotometer with a fixed slit width (2 nm) connected to an IBM compatible computer and a HP 600 inkjet printer was used. The bundled software was UV PC personal spectroscopy version 3.91 (Shimadzu). MaterialsMB, SU and Colona tablets containing 100 mg MB and 25 mg SU per tablet, were kindly supplied by Rameda (6 October City, Egypt). Analytical grade methanol and hydrochloric acid were used throughout these experiments. Standard solutionsStock solutions of MB and of SU were prepared separately by dissolving 100 mg and 10 mg of each drug, respectively, in 100 ml methanol. The standard solutions were prepared by dilution of the stock solutions with 0.1 M HCl to reach a concentration range of 4 -20 and 1 -10 µg/ml for MB and SU, respectively. Faculty of Industrial Education, Beni Suef, EgyptTwo methods are described for the simultaneous determination of mebeverine hydrochloride (MB) and sulpiride (SU) in their combinations. The first method depends on the first derivative of the ratio spectra by measurement of the amplitudes at 263.7 and 234.9 nm for MB and SU, respectively. The linear ranges and detection...

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

“…MEB and SUL were determined in their binary mixture via derivative spectroscopy [3,4], first derivative synchronous fluorescence spectroscopy [5]. TLC- densitometry [4], HPLC [4-6] and chemometric techniques [7].…”

Spectrofluorimetric method for the determination of sulpiride in pharmaceutical preparations and human plasma through derivatization with 2‐cyanoacetamide

“…These methods include gas chromatography (GC) (9,10), spectrophotometry (11,12), high-performance liquid chromatography (HPLC) with UV detection (13)(14)(15), HPLC with fluorescence detection (16,17), HPLC-mass spectrometry (MS) (18), GC-MS (19), capillary electrophoresis (20,21) and adsorptive stripping voltammetry (22). These methods include gas chromatography (GC) (9,10), spectrophotometry (11,12), high-performance liquid chromatography (HPLC) with UV detection (13)(14)(15), HPLC with fluorescence detection (16,17), HPLC-mass spectrometry (MS) (18), GC-MS (19), capillary electrophoresis (20,21) and adsorptive stripping voltammetry (22).…”

“…A number of analytical methods have been published in the literature for the analysis of sulpiride in pharmaceutical formulations and biological fluids. These methods include gas chromatography (GC) (9,10), spectrophotometry (11,12), high-performance liquid chromatography (HPLC) with UV detection (13)(14)(15), HPLC with fluorescence detection (16,17), HPLC-mass spectrometry (MS) (18), GC-MS (19), capillary electrophoresis (20,21) and adsorptive stripping voltammetry (22). An oscillopolarographic method has also been reported for the determination of sulpiride in the presence of acetic acid:sodium acetate buffer at pH 5 (23).…”

Development of Membrane Selective Electrode for Determination of the Antipsychotic Sulpiride in Pharmaceuticals and Urine

“…A review of the literature revealed that several analytical methods have been described for the determination of sulpiride in pharmaceuticals or biological fluids, including spectrophotometric [4-7], fluorimetric [8], chromatographic [9-14], electrophoretic [15-17], voltammetric [18] and chemiluminometric [19]; however, the methods proposed for the analysis of biological fluids suffer the inconvenience of time-consuming procedures and expensive instrumentation.…”