Simultaneous determination of loratadine and desloratadine in pharmaceutical preparations using liquid chromatography with a microemulsion as eluent

El-Sherbiny, Dina T.; El‐Enany, N.; Belal, F.; Hansen, Steen Ingemann

doi:10.1016/j.jpba.2006.10.027

Cited by 63 publications

(25 citation statements)

References 26 publications

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“…Samples were analyzed using a Luna Silica 50 mm × 2.0 mm, 5 m particle size, analytical column, Phenomenex (Torrance, CA) along with a Gemini C 18 Table 1.…”

Section: Gradient Methods For the Analysis Of Endogenous Componentsmentioning

confidence: 99%

“…Chlorpheniramine has both a proton acceptor site and a electron donor site and represents a good example compound for possible analyte-endogenous matrix component ion suppression interactions and was selected in this study as a test compound. Loratadine, ethyl-4-(8-chloro-5,6 dihydro-11H-benzo[5,6] cyclohepta[1,2b]pyridin-11-ylidene)-1-piperidine carboxylate (pKa 5.0) [18] has a pyridine nitrogen atom, a different pKa and was chosen as a second confirmatory test compound.…”

Section: Post-column Infusionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry

Ismaiel

Zhang

Jenkins

et al. 2010

Journal of Chromatography B

134

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“…Samples were analyzed using a Luna Silica 50 mm × 2.0 mm, 5 m particle size, analytical column, Phenomenex (Torrance, CA) along with a Gemini C 18 Table 1.…”

Section: Gradient Methods For the Analysis Of Endogenous Componentsmentioning

confidence: 99%

Section: Post-column Infusionmentioning

confidence: 99%

Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry

Ismaiel

Zhang

Jenkins

et al. 2010

Journal of Chromatography B

134

View full text Add to dashboard Cite

“…Desloratadine is partially soluble in water and completely soluble in ethanol [21]. Literature survey revealed that spectrophotometry [22], RP-HPLC [23][24][25] methods were used for estimation of desloratadine in combination with other drugs.…”

Section: Montelukast (Fig 1) Chemically Is (1-[[[1r-1-[3-(1e)-2-(7-cmentioning

confidence: 99%

RP-HPLC-PDA Method for Simultaneous Quantification of Montelukast, Acebrophylline and Desloratadine Tablets

Mamatha¹,

Devanna²

2018

Asian J. Chem.

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A simple, fast, accurate and specific RP-HPLC-PDA method has been developed for the simultaneous quantification of montelukast, acebrophylline and desloratadine in bulk and tablet dosage form. The chromatographic separation was performed on a reverse phase BDS C8 Column (150 × 4.6mm, 5 µm particle size) consisting mobile phase of potassium hydrogen ortho-phosphate buffer:acetonitrile (40:60 v/v), with a flow rate 1 mL/min, temperature 30 ºC and UV detection wavelength 280 nm. The retention times of montelukast, acebrophylline and desloratadine were observed as 2.04, 2.68 and 3.77 min, respectively. The developed method was validated by validation parameters such as linearity, range, accuracy, precision and robustness. The results obtained for validation parameters are within the limits as per ICH guidelines. The linearity of the drugs were obtained in the range of 5-30 ppm for montelukast, 100-600 ppm acebrophylline and 2.5-15 ppm for desloratadine. % RSD from precision studies were 0.4, 0.1 and 1.0 mean percentage recovery from accuracy studies were found to be 98.47, 98.71 and 100.11 for montelukast, acebrophylline and desloratadine respeactively. Limit of detection values were found as 0.01, 0.32 and 0.01 for montelukast, acebrophylline and desloratadine, respectively. Limit of quantification values were found as 0.03, 0.98 and 0.04 montelukast, acebrophylline and desloratadine, respectively. The method designed and validated can be successfully used for the regular quantification of montelukast, acebrophylline and desloratadine in tablet and bulk forms.

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“…The evaluation of the operating parameters in order to assess its capabilities in pharmaceutical analysis was performed. [4][5][6][7][8][9][10] Also, the potential of MELC is proved by its expansion to the optimization of microemulsion mobile phase composition in order to model the drug penetration across blood-brain barrier. 11 Apropos the selected substances, they were previously investigated by the same authors employing RP-HPLC in order to determine their pKa values, as well as for the method optimization and robustness testing.…”

Section: Definition Of the Investigation Objectivementioning

confidence: 99%

Physicochemical factors governing the partition of pramipexole and its five impurities in microemulsion liquid chromatographic systems

Vemić¹,

Malenović²,

Rakić³

et al. 2012

J. Braz. Chem. Soc.

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As microemulsões, quando usadas como fase móvel, representam uma ferramenta cromatográfica poderosa devido à sua estrutura polifásica. O objetivo deste estudo foi investigar a influência da composição da fase móvel e as propriedades das gotículas resultantes da microemulsão, bem como a lipofilicidade da fase estacionária no comportamento cromatográfico de pramipexol e suas cinco impurezas. A investigação foi realizada de acordo com o plano experimental definido pelo planejamento fatorial completo 2 4 com quatro repetições do ponto central. Embora o mais afetado pelas características da coluna tenha sido o último eluente, a substância mais lipofílica, a composição e as características das gotículas de microemulsão foram igualmente importantes, de modo que o sistema de partição gotículas de microemulsão/fase estacionária provou ser o mais significativo. A lipofilicidade da fase estacionária não afetou o componente mais hidrofílico e somente o sistema de partição gotículas de microemulsão/volume do eluente causou impacto em seu comportamento. Para a separação do par crítico, foram notados efeitos quase iguais dos sistemas de partição gotículas de microemulsão/fase estacionária e volume do eluente/fase estacionária.Microemulsions when used as mobile phases represent a very powerful chromatographic tool due to their polyphase structure. The aim of this study was to investigate the influence of the mobile phase composition and resulting microemulsion droplet's properties, as well as the stationary phase lipophilicity on the chromatographic behavior of pramipexole and its five impurities. Investigation was carried out according to the experimental plan defined by a full factorial design 2 4 with four central point replications. While the most affected by column characteristics was the last eluting, most lipophilic substance, the composition and characteristics of microemulsion droplets were equally important, so the partition system microemulsion droplets/stationary phase proved to be the most significant. Stationary phase lipophilicity did not affect the most hydrophilic component and only the partition system microemulsion droplets/bulk of the eluent impacted its behavior. For the separation of the critical pair, almost equal effects of the microemulsion droplets/stationary phase and bulk of the eluent/stationary phase partition systems were noted.

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Simultaneous determination of loratadine and desloratadine in pharmaceutical preparations using liquid chromatography with a microemulsion as eluent

Cited by 63 publications

References 26 publications

Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry

Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry

RP-HPLC-PDA Method for Simultaneous Quantification of Montelukast, Acebrophylline and Desloratadine Tablets

Physicochemical factors governing the partition of pramipexole and its five impurities in microemulsion liquid chromatographic systems

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