Simultaneous determination of hydroxysafflor yellow A and ferulic acid in rat plasma after oral administration of the co‐extractum of Rhizoma chuanxiong and Flos Carthami by HPLC–diode array detector

Abstract

A simple, rapid and accurate HPLC method has been developed for simultaneous determination of hydroxysafflor yellow A and ferulic acid in rat plasma after oral administration of the co-extractum of Rhizoma chuanxiong and Flos Carthami. Plasma samples were deproteinized with 6% perchloric acid, and riboflavin was used as internal standard. The supernatant after centrifuge was injected into a Shimadzu C(18) (150 x 4.6 mm, i.d. 5 microm) column. Gradient elution for A:B (0 min, 90:10; 25 min, 70:30; 27 min, stop) was applied. The mobile phase was composed of 0.022 mol/L potassium dihydrogen phosphate solutions, adjusted to pH 3.0 with phosphoric acid for pump A, and 90% (v/v) acetonitrile for pump B. The assay was shown to be linear over the range 0.046-4.6 microg/mL (r(2) = 0.9995) for hydroxysafflor yellow A and 0.037-3.7 microg/mL (r(2) = 0.9998) for ferulic acid. Mean recovery was 97.5% for hydroxysafflor yellow A and 83.6% for ferulic acid. Both of the intra-day and inter-day precisions were <or=8.1%. The limits of quantification of hydroxysafflor yellow A and ferulic acid were 0.046 and 0.037 microg/mL, respectively. The HPLC method was successfully applied to the pharmacokinetic studies of hydroxysafflor yellow A and ferulic acid in rat plasma after oral administration of the co-extractum of Rhizoma chuanxiong and Flos Carthami.

Simultaneous determination of six hydrophilic components in rat plasma after oral administration of Jitai tablet by liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to a pharmacokinetic study

“…This prediction proved to be correct based on the obtained results, which was also in conformity with the literature reference that intestinal absorption of intact ADL was rather limited [18]. It was reported that the absorption of HSYA was rapid with peak concentrations occurring at 10 min after oral administration [40]. As calculated from the mean plasma concentrations of HSYA following oral administration of JTT, the absorption speed of HSYA was rapid with highest plasma concentration observed (T max ) at 0.31 ± 0.12 h (T max less than 1 h).…”

Enhanced effect and mechanism of water-in-oil microemulsion as an oral delivery system of hydroxysafflor yellow A

“…Chromatographic analysis was performed as previously described, 22 with some modifications. A Shim-pack CLC-ODS C18 (150 × 4.6 mm, internal diameter, 5 µm) HPLC column (Shimadzu, Kyoto, Japan) was used.…”

A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the determination of hydroxysafflor yellow A in human plasma: Application to a pharmacokinetic study

“…Some high-performance liquid chromatography methods employing UV detection have previously been developed to determine HSYA in rat plasma [8,9]. However, the lower limit of quantification (LLOQ ≥ 0.046 g/ml) of those methods coupled with UV detection is too high to monitor the therapeutic levels of safflower oral solution.…”