A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the determination of hydroxysafflor yellow A in human plasma: Application to a pharmacokinetic study

Simultaneous determination of six hydrophilic components in rat plasma after oral administration of Jitai tablet by liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to a pharmacokinetic study

“…Various analytical methods based on liquid chromatography technology have been developed for the determination of the six compounds in biological fluids, respectively [18][19][20][21][22][23][24]. However, these methods have shortens: high limit of quantification, long analysis time, or could not fulfill oral PK study due to the insufficient sensitivity and specificity.…”

Determination of hydroxysafflor yellow A in biological fluids of patients with traumatic brain injury by UPLC‐ESI‐MS/MS after injection of Xuebijing

“…The measured therapeutic plasma concentrations ranged from 4350.3 to 5431.1 ng/mL for HSYA. The results seem to be in agreement with those reported previously (Wen et al ., ). According to results of CSF determination, the CSF‐to‐plasma ratio ranged from 0.45 to 1.02% after 4 h, which indicated that, even under the state of blood–brain barrier disruption, relatively a low concentration of HSYA in CSF can keep Xuebijing injection safe after intravenous injection in the treatment of TBI patients.…”

“…However, whether and which anthraquinones contained in the five bioactive components of XBJ are absorbed by TBI patients remain unclear. Analytical methods have been developed for the determination of HSYA in human plasma, rat plasma, rat intestines and rat lung (Li et al ., , ; Wen et al ., ). These include high‐performance liquid chromatography (HPLC) and HPLC coupled with tandem mass spectrometry (HPLC‐MS/MS).…”

Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

“…In previous reports (Li et al, ; Liu et al, ; Wen, Qi, et al, ; Wen, Yang, et al, ), most of researchers applied solid‐phase extraction in sample preparation to obtain better sensitivity for analysis of HSYA, ASIV, CAG, calycosin, C‐3′‐G and C‐3′‐S. This technique usually uses a larger sample volume (200 μL) and its operation is more complex.…”

“…Some LC–MS/MS methods were developed for simultaneous analysis of CAG, calycosin and/or ASIV as well as other components in bio‐samples (Liu et al, ; Shaw, Lin, & Tsai, ; Shi et al, ). Some LC–MS/MS methods were established to quantify HSYA with/without other compounds in rat or human plasma (Jin et al, ; Li et al, ; Wang, Liu, et al, ; Wen, Yang, et al, ). In the published LC–MS/MS methods, CAG, calycosin and ASIV were detected most frequently in positive ionization mode while HSYA was monitored in negative mode.…”