Simultaneous determination of galanthamine and lycorine in Lycoris radiata by a capillary electrophoresis with an electrochemiluminescence method

Abstract

A novel capillary electrophoresis with electrochemiluminescence determination method was developed for the determination of two alkaloids based on the electrochemiluminescence signal enhancement effect of the tertiary amine group on tris(2,2'-bipyridyl)ruthenium(II). A linear relationship between the electrochemiluminescence peak area and concentrations of galanthamine and lycorine in the range of 0.07 ∼ 17 μg/mL and 0.07 ∼ 18 μg/mL was obtained and the detection limit was 0.008 and 0.002 μg/mL, respectively. The method is selective, simple, and convenient. It had been successfully applied to the analysis of galanthamine and lycorine in Lycoris radiata samples purchased from a local market.

Recent advances in the analysis of plant alkaloids by capillary electrophoresis and micellar electrokinetic chromatography

“…Borate buffer is also used in CZE analysis of analytes as this buffer interacts with polyhydroxy molecules results in enhancement of absorbance by UV detection. Analysis of nortropane polyhydroxylated alkaloids calystegines isolated from a different plant family such as Convolvulaceae , Moraceae and, Solanaceae , using tetraborate (80 mM) as BGE at pH 9.2 permitted good UV detection in the CZE method [27].…”

Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector

“…There are many methods for the determination of galantamine, including high‐performance liquid chromatography (HPLC) . The determination of galantamine in dosage form using the UV–photometric method has also been described …”

“…[1] The mean maximum plasma drug concentration (C max ) and time to reach C max (t max ) after a single oral (tablet) 10 mg dose of galantamine are 49.2 μg/L and 0.88 h. [2] There are many methods for the determination of galantamine, including high-performance liquid chromatography (HPLC). [3][4][5][6][7][8][9][10][11][12][13][14][15] The determination of galantamine in dosage form using the UV-photometric method has also been described. [7] This study examined a new spectrofluorimetric method for the determination of galantamine in plasma and urine.…”

Total monitoring of the constituents of Danshen tablet using micellar electrokinetic chromatography fingerprinting for antioxidant activity profiling

“…By contrast, CE is an economical method with many valuable advantages such as short analysis time, simple sample pretreatment, minimal solvent consumption, high peak capacity, high resolution and excellent mass sensitivity [2,4,5,[7][8][9][10][11][12]. Thus, CE has gradually become a powerful tool in separation science and achieved better separation performances in TCM [7][8][9][10][11][12][13][14][15][16][17]. There are multiple separation modes in CE technique: CZE, MEKC, ACE, and non-gel CE, etc.…”