Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector

Abstract

A new method based on fluorescence derivatization with 5-(dimethylamino) naphthalene-1-sulfonyl chloride (dansyl chloride) was developed for the quantitative determination of galantamine in human plasma and urine using high-performance liquid chromatography. The reaction between galantamine and dansyl chloride was optimally realized in 30 min at room temperature and pH 10.5, with a reagent to galantamine molar ratio of 2.13. The derivative was extracted with dichloromethane, and the extract was dried under a nitrogen stream and dissolved in the mobile phase. Chromatographic analysis was performed with an Inertsil C column and a mobile phase comprising 40% acetonitrile and 60% 10 mM o-phosphoric acid, 1.2 ml/min. The injection volume was 20 μl. The derivatives were detected with a fluorescence detector (excitation 375 nm/emission 537 nm). The retention time for the dansyl derivative of galantamine was 16.8 min. Linearity was observed between 125 and 2000 ng/ml in water, urine and plasma. The limit of detection and limit of quantification for the developed method were 6.27-70.99 and 18.81-212.97 ng/ml, respectively. Per cent recovery was calculated as 95.15 for urine and 95.78 for plasma. Interday repeatability values for urine and plasma samples (n = 6) at three different concentrations were calculated as a per cent relative standard deviation of 0.24-0.59 and 0.35-0.56. The corresponding per cent relative standard deviation values for intraday repeatability were 0.13-0.51 and 0.04-0.15, respectively.

Graphene Ink Modified Glassy Carbon Electrode as electrochemical sensor for Galantamine Determination

“…Galantamine can improve the conduction between nerve and muscle. Compared with physostigmine, neostigmine and piazimine, galantamine has a wide therapeutic range, low toxicity and weak muscarinic action [2,3]. Galantamine is mainly used in the treatment of myasthenia gravis, poliomyelitis and sequelae but also for children with cerebral palsy, polyneuritis, spinal radiculitis, and nervous system diseases [4].…”

Astemizole-based turn-on fluorescent probes for imaging hERG potassium channel

“…In addition, some fluorophores with good fluorescent properties could be used in small-molecule fluorescent probes, such as naphthalimide 18 and dansyl derivative. 19 In order to keep the inhibitory activity of the probe, the major interaction sites of astemizole's bonding were retained. Herein, the naphthalimide and dansyl fluorophores were conjugated to the recognition moiety using an aliphatic spacer.…”