Total monitoring of the constituents of Danshen tablet using micellar electrokinetic chromatography fingerprinting for antioxidant activity profiling

Abstract

A micellar electrokinetic chromatography fingerprint coupled with quantification was developed for assessing the quality consistency of Danshen tablets, a traditional Chinese medicine, with pharmacological activities of activating blood to remove blood stasis and regulating the flow of vital energy to alleviate pain. Square optimization method was proposed to select the suitable background electrolyte. Subsequently, the index of chromatographic fingerprints F was simultaneously employed as an objective function to evaluate capillary electrophoresis conditions. After that, the method was proved to meet the fingerprint analysis criteria by evaluating stability, linearity, limit of detection, limit of quantification, precision, accuracy, and repeatability. Moreover, systematic quantified fingerprint method was used to evaluate chromatographic fingerprints of 30 batches of samples in terms of both quality and quantitation. The result indicated that the chemical compositions of samples were basically similar while their contents showed marked variation. In addition, the fingerprint-efficacy relationship was established using partial least squares model, which can serve as anti-oxidant activity evaluation of Danshen tablets as well as a reference for the selection of active constituents. The proposed method can be applied for the holistic quality control of Danshen tablets.

Assessment of quality consistency in traditional Chinese medicine using multi‐wavelength fusion profiling by integrated quantitative fingerprint method: Niuhuang Jiedu pill as an example

“…First, sample and reference fingerprint vectors are respectively converted into and , the cosine of the two vectors after the conversion is denoted as the qualitative ratio similarity (), as calculated by Eq. . This conversion makes all the peaks in the same range.…”

Simultaneous separation and determination of praeruptorin A, B and C by micellar electrokinetic chromatography using sodium dodecyl sulphate and sodium cholate as mixed micelles

“…The concentration of the running buffer can affect ionic strength which in turn will affect separation behaviour including migration time, resolution, peak shape and running current. In this work, different concentrations of borate solution (15,20 and 25 mM) were tested with Typically, pH of running buffer plays a prominent role in the separation selectivity as it affects both analyte ionisation and the velocity of EOF. In this study, the effect of pH on the separation was explored in the range between 8.00 and 10.50 (at intervals of 0.50).…”

“…For the past decades, capillary electrophoresis (CE) has been increasingly regarded as a promising technique for the quality control of herbal drugs because it introduces numerous attractive advantages such as high resolving power, short analysis time, less consumption of solvents, ultra-small sample requirements, simple preparation of sample and low operating cost. 15 These features are beneficial in separating natural compounds with complicated matrices. 16 CE is capable of analysing different types of analytes according to their molecular weight, charge amount in buffer solution, hydrophobicity and chirality by its various modes of operations.…”

Capillary electrophoresis fingerprints combined with chemometric methods to evaluate the quality consistency and predict the antioxidant activity of Yinqiaojiedu tablet

“…PLS is a supervised technique. It has been used to find the fundamental relation between CE fingerprint and biological activity . Based on these, chemometric methods have been increasingly applied to CE fingerprints analysis in support of the QC of TCM.…”