Simultaneous determination of eight components in Angelica sinensis based on UHPLC‐ESI‐MS/MS method for quality evaluation

Abstract

A method employing ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for determination of eight components including ferulic acid, senkyunolide A, butylphthalide, ligustilide, butylidenephalide, senkyunolide I, senkyunolide H and levistolide A in Angelica sinensis was established. The separation was carried out using a Waters ACQUITY UHPLC BEH C column with gradient elution with 0.1% formic acid aqueous and acetonitrile at a flow rate of 0.4 mL/min. Good linearity was attained with R of 0.9983-0.9998 in wide concentration ranges. The method had limit of detection (LOD) and quantitation (LOQ) in the range of 0.42-6.98 ng/mL and 1.39-23.28 ng/mL, respectively. Intra- and inter-day precisions varied with relative standard deviations (RSDs) from 0.33% to 0.88% and 0.37% to 1.04%, respectively. Moreover, the average recoveries were in a satisfactory range of 92.7%-102.1% with RSDs of less than 3.60%. Finally, the method was successfully applied to analyze 19 batches of A. sinensis samples grown in Min County, Gansu province, China, as well as that collected in other regions. The findings indicated that the established method is reliable and may thus be applied as a powerful tool for qualitative and quantitative analysis of components in A. sinensis, which has its implications in quality control of A. sinensis.

Effects of Tokishakuyakusan on Regeneration of Murine Olfactory Neurons In Vivo and In Vitro

“…However, we examined β-eudesmol at 100 μmol/L and found out no activity, as shown in Supplementary Figure S1. Levistolide A, the other NGF-increasing active ingredient identified in this study, is found in CR (Zuo et al 2011) and JAR (Gui and Zheng 2019). However, CR was unable to increase NGF in our study, as the concentration in the CR extract might have been low.…”

“…In addition, to identify NGF enhancers in TSS, 30 compounds present in TSS were examined at a single concentration (100 μmol/L); the results are shown in Supplementary Figure S1. Atractylodin, which is present in ALR (Chen et al 2012), and levistolide A, which is present in CR (Zuo et al 2011) and JAR (Gui and Zheng 2019), significantly enhanced NGF production in astrocytes. These active ingredients further showed concentration-dependent actions (Figure 8).…”

Integrated Phytochemical Analysis Based on UPLC–MS/MS and Network Pharmacology Approaches to Explore the Effect of Odontites vulgaris Moench on Rheumatoid Arthritis

“…Melampyroside is an iridoid compound, and its relative peak areas in the ethyl acetate and n-butanol extracts reached 11.39% and 13.31%, respectively. Iridoids have been studied for their anti-inflammatory activity (Gui and Zheng, 2018). Taken together, the four selected compounds were representative and formed a basis for the verification experiment.…”

Modular Characteristics and Mechanism of Action of Herbs for Endometriosis Treatment in Chinese Medicine: A Data Mining and Network Pharmacology–Based Identification

“…levistolide A (Gui and Zheng, 2019). The volatile oil of A. sinensis, has evident antiinflammatory activities (Zhong et al, 2016).…”