Integrated Phytochemical Analysis Based on UPLC–MS/MS and Network Pharmacology Approaches to Explore the Effect of Odontites vulgaris Moench on Rheumatoid Arthritis

Ji, Minjun; Wang, Congcong; Yang, Tieyi; Meng, Xiangxi; Wang, Xiaoqin; Li, Min-Hui

doi:10.3389/fphar.2021.707687

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…[41], respectively. In the recent literature, it has been reported that compound 4 has anti-inflammatory activity [42], along with other iridoids not reported herein, which linked to a study on the potential activity of Odontites vulgaris against rheumatoid arthritis. In an even earlier study [43] including other iridoids and compound 4, the latter was also found to be cardioactive in Wistar rats.…”

Section: Resultsmentioning

confidence: 62%

Iridoid Glycosides Isolated from Bellardia trixago Identified as Inhibitors of Orobanche cumana Radicle Growth

Soriano

Siciliano

Fernández‐Aparicio

et al. 2022

Toxins

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Orobanche cumana is an obligate holoparasitic plant with noxious effects in sunflower crops. Bellardia trixago is a facultative hemiparasitic plant that infects ruderal plants without noxious significance in agriculture and is known to produce a wide spectrum of bioactive metabolites. The objective of this study was to evaluate the allelopathic effects of B. trixago on the growth of O. cumana seedlings. Three different extracts using solvents of increasing polarity (n-hexane, dichloromethane and ethyl acetate) were prepared from the flowers, aerial green organs and roots of two populations, a white-flowered and a yellow-flowered population of B. trixago, both collected in southern Spain. Each extract was studied using allelopathic screenings on O. cumana which resulted in the identification of allelopathic activity of the ethyl acetate extracts against Orobanche radicles. Five iridoid glycosides were isolated together with benzoic acid from the ethyl acetate extract of aerial green organs by bio-guided purification. These compounds were identified as bartsioside, melampyroside, mussaenoside, gardoside methyl ester and aucubin. Among them, melampyroside was found to be the most abundant constituent in the extract (44.3% w/w), as well as the most phytotoxic iridoid on O. cumana radicle, showing a 72.6% inhibition of radicle growth. This activity of melampyroside was significantly high when compared with the inhibitory activity of benzoic acid (25.9%), a phenolic acid with known allelopathic activity against weeds. The ecotoxicological profile of melampyroside was evaluated using organisms representing different trophic levels of the aquatic and terrestrial ecosystems, namely producers (green freshwater algae Raphidocelis subcapitata and macrophyte Lepidium sativum), consumers (water flea Daphnia magna and nematode Caenorhabditis elegans) and decomposers (bacterium Aliivibrio fischeri). The ecotoxicity of melampyroside differed significantly depending on the test organism showing the highest toxicity to daphnia, nematodes and bacteria, and a lower toxicity to algae and macrophytes. The findings of the present study may provide useful information for the generation of green alternatives to synthetic herbicides for the control of O. cumana.

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Section: Resultsmentioning

confidence: 62%

Iridoid Glycosides Isolated from Bellardia trixago Identified as Inhibitors of Orobanche cumana Radicle Growth

Soriano

Siciliano

Fernández‐Aparicio

et al. 2022

Toxins

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“…The main compositions of Khurtsiin Deed-6 traditional medicine are Odontites Vulgaris Moench and Carthamus tinctorius L, and they contain apigenin, flavonol-O-glycoside (hyperoside, isoquercetin), flavonoids (quercetin, kaempferol, rutin) and Carthamus tintorius L. also contains hydroxysafflor yellow A. 4,24,25 The rhizome of Saussurea lappa L. contains biologically active compounds such as sesquiterpene, costunolide, and dihydrocostulactone. 26 Some study results of Saussurea lappa L. showed that costunolide can be an inhibitor of NO, iNOS, and TNF-a.…”

Section: Discussionmentioning

confidence: 99%

“…The compounds are mainly divided into four categories: phenylethanoid glycosides (verbascoside, isoacteoside, arenarioside, salidroside), flavonoids (kaempferol, cynaroside, luteolin, luteolin-7-O-glucuronide, apigenin, apigenin-7-O-glucoside, tricin, chrysoeriol, quercetin, diosmetin, hydroxygenkwanin), iridoids (melampyroside, shanzhiside methyl ester, aucubin, geniposide, geniposidic acid, shanzhiside methyl ester), phenolic acids (caffeic acid, trans-cinnamic acid, 2-hydroxycinnamic acid, salicylic acid, ferulic acid, 7,8-dihydroxycoumarin) and others (adenosine, syringaresinol, D-Mannitol, esculetin). 4 Numerous researches conducted on T.chebula have confirmed the presence of wide range of the phytochemicals such as flavonoids, tannins, phenolic acids and other bioactive compounds. Juang et al (2004) developed an reverse-phase HPLC method for the determination of 14 phenolic components of hydrolyzable tannins (gallic acid, chebulic acid, chebulanin, corilagin, punicalagin, neochebulinic acid, ellagic acid, chebulegic acid, chebulinic acid,1,2,3,4,6-Penta-O-galloyl-β-D-glucose, 1,6,-di-O-galloyl-D-glucose, casuarinin, 3,4,6-tri-O-galloyl-D-glucose, terchebulin) in the fruits of T.chebula.…”

Section: Introductionmentioning

confidence: 99%

Standardization Study of Khurtsiin deed-6 Traditional Medicine

Myadagbadam¹,

Purevsuren²,

CH³

et al. 2022

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Introduction:Although traditional medicines are widely used, quality and safety of some of them are not ensured due to lack of suitable quality controls and inadequate labeling. he diagnostic cellular structures and cell content for all powdered ingredients in Khurtsiin deed-6 traditional medicine were defined by "Novel" light microscopy. Biologically active compounds were identified by using TLC and HPLC. Main biologically active compounds contents were determined by UV spectrophotometer methods. Some quality and safety parameters of Khurtsiin deed-6 traditional medicine were determined by Mongolian National First Pharmacopoeia methods. Results: Some quality and safety parameters of the traditional medicine were determined as: moisture 9.63±0.09%, total ash 4.725±0.22%, and water-soluble extractive 21.28±0.11%, Alcohol soluble extractive total 46.30±0.09%, aerobic microbial count 5x10 3 , total yeast and mold count 3x10 2 . The fingerprints of TLC and HPLC to reveal gallic acid, apigenin, costunolide, E-guggulsterone were defined. The total contents were measured for phenolic compounds as 65.95±1.05 mg/g and for flavonoids as 10.55 ±0.16 mg/g. Conclusion: The standardization criteria for Khurtsiin deed-6 traditional medicine were defined and Mongolian National Pharmacopeia Monograph's draft for Khurtsiin deed-6 traditional medicine was developed.

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“…Generally, a binding energy of ≤ 5.0 kcal/mol indicates a good binding effect. PyMol 2.3.2 software was used for visualization ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of Koumiss on Intestinal Immune Modulation in Immunosuppressed Rats

Zhang

Xilin

et al. 2022

Front. Nutr.

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Koumiss is a traditional fermented dairy product with health and medicinal benefits. It is very popular in the Inner Mongolia Autonomous Region of China. The results of relevant studies have shown that koumiss can regulate the gastrointestinal environment, improve the absorption of nutrients, improve the body's intolerance to lactose, enhance the body's immunity, prevent scurvy and atherosclerosis, and aid in the treatment of tuberculosis. However, there are no systematic reports on the effects of koumiss on immunity. In this study, we aimed to decipher the effects of koumiss on intestinal immune modulation. We used liquid chromatography-tandem mass spectrometry (LC-MS) analysis to determine the composition of Koumiss. Using Compound Discoverer software, we compared the mass spectrometry data with the compound information in the online databases ChemSpider and mzCloud to intelligently identify the main chemical components of koumiss. Additionally, we used Mass Frontier small molecule fragmentation libraryTM to determine the structure of fragment ions. A total of 21 components were identified, which clarified the chemical basis of koumiss. These 21 compounds were then used to perform molecular docking with immune-related targets, such as TNF, IL2, IL10, etc. The results indicated good docking activity between most of the compounds and the targets. Then, an immunosuppressive rat model was used to determine the therapeutic effect of koumiss. The results of this study showed that koumiss could, to a certain extent, correct the atrophy of the thymus and spleen in immunosuppressed model rats. The number of leukocytes, lymphocytes, and the CD4+/CD8+ ratio of peripheral blood lymphocytes was also increased. In addition, it could effectively improve the structure of the small intestinal mucosa, which shows that koumiss has a positive effect on the intestinal immune function of immunosuppressed rats. These findings provide an experimental basis for the development and utilization of koumiss as a therapeutic product.

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Integrated Phytochemical Analysis Based on UPLC–MS/MS and Network Pharmacology Approaches to Explore the Effect of Odontites vulgaris Moench on Rheumatoid Arthritis

Cited by 7 publications

References 61 publications

Iridoid Glycosides Isolated from Bellardia trixago Identified as Inhibitors of Orobanche cumana Radicle Growth

Iridoid Glycosides Isolated from Bellardia trixago Identified as Inhibitors of Orobanche cumana Radicle Growth

Standardization Study of Khurtsiin deed-6 Traditional Medicine

Effects of Koumiss on Intestinal Immune Modulation in Immunosuppressed Rats

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