Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC–MS–MS method

Tao, Wuqun; Zhou, Zhiguang; Zhao, Bin; Wei, Tongyu

doi:10.1016/j.jpba.2016.08.020

Cited by 73 publications

(59 citation statements)

References 32 publications

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“…The contents of main compounds of teas are highly depended on tea types, and also on the tea grades (Tao, Zhou, Zhao, & Wei, 2016). In the same tea type, the variation of polyphenols can also be affected by geographical factors.…”

Section: Tea Chemistry Main Composition Of Various Teasmentioning

confidence: 99%

Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review

Liang

Zhou

et al. 2019

Comp Rev Food Sci Food Safe

314

237

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Tea is a typical processed beverage from the fresh leaves of Camellia sinensis [Camellia sinensis (L.) O. Kuntze] or Camellia assamica [Camellia sinensis var. assamica (Mast.) Kitamura] through different manufacturing techniques. The secondary metabolites of fresh tea leaves are mainly flavan‐3‐ols, phenolic acids, purine alkaloids, condensed tannins, hydrolysable tannins, saponins, flavonols, and their glycoside forms. During the processing, tea leaves go through several steps, such as withering, rolling, fermentation, postfermentation, and roasting (drying) to produce different types of tea. After processing, theaflavins, thearubigins, and flavan‐3‐ols derivatives emerge as the newly formed compounds with a corresponding decrease in concentrations of catechins. Each type of tea has its own critical process and presents unique chemical composition and flavor. The components among different teas also cause significant changes in their biological activities both in vitro and in vivo. In the present review, the progress of tea chemistry and the effects of individual unit operation on components were comprehensively described. The health benefits of tea were also reviewed based on the human epidemiological and clinical studies. Although there have been multiple studies about the tea chemistry and biological activities, most of existing results are related to tea polyphenols, especially (‐)‐epigallocatechin gallate. Other compounds, including the novel compounds, as well as isomers of amino acids and catechins, have not been explored in depth.

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Section: Tea Chemistry Main Composition Of Various Teasmentioning

confidence: 99%

Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review

Liang

Zhou

et al. 2019

Comp Rev Food Sci Food Safe

314

237

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“…In the MS spectrum of epigallocatechin, the characteristic fragments of m/z 179.0325 and 125.0234 were formed due to the cleavage of B ring and C ring at ( 1,4 A − ) directly from the parent ion [M–H] – at m/z 305.0660. Epigallocatechin gallate gave the [M–H] − ion at m/z 457.0766; the fragments at m/z 305.0658 were detected due to the loss of galloyl group in negative mode, while the fragment at m/z 169.0129 corresponded to the gallic acid unit .…”

Section: Resultsmentioning

confidence: 99%

Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba

Gao²,

et al. 2017

J. Sep. Sci.

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Ardisiae Japonicae Herba is a well-known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high-performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.

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“…In the study, the developed method for simultaneous determination of seven oligostilbenes in P. ostii seed shell by HPLC‐DAD was validated using metrics including calibration linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, repeatability and recovery . Linearity of standards was determined from the relationship between the peak area and concentration over the five concentration ranges of the working solutions.…”

Section: Methodsmentioning

confidence: 99%

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Zhang

Liu

Gao

et al. 2019

Phytochemical Analysis

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Introduction The Paeonia ostii T. Hong & J. X. Zhang seed shell, characterised by a high content of oligostilbenes, is one of the two most important by‐products in the preparation of seed oil. Oligostilbenes are considered characteristic constituents of the genus Paeonia, and can be used in fingerprinting to determine the geographical origin and the quality of raw materials. Objective To develop and optimise a simple and reproducible high‐performance liquid chromatography diode array detection (HPLC‐DAD) method for the simultaneous determination of seven oligostilbenes in P. ostii seed shell from different geographical areas, and to associate the cultivation area. Methodology A validated HPLC method coupled with a DAD detector was performed for the detection and determination of target compounds in the samples. Optimal chromatographic conditions were achieved using an Agilent Zorbax Eclipse SB‐AQ‐C18 column and a gradient elution with acetonitrile and potassium dihydrogen phosphate solution. Results The proposed quantitative method showed appropriate accuracy and precision, and was successfully applied to the routine analysis of seven oligostilbenes and the quality evaluation of 50 P. ostii seed shell samples. There were significant differences between the contents of the seven oligostilbenes in different samples (P < 0.01). Conclusion The results demonstrated that the oligostilbenes were main secondary metabolites in the P. ostii seed shells, and the content of seven components in P. ostii seed shells sourced from different cultivation areas in China was different.

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Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC–MS–MS method

Cited by 73 publications

References 32 publications

Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review

Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review

Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

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