Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba

Abstract

Ardisiae Japonicae Herba is a well-known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high-performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.

Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components

“…Flavonoids are mainly as follows: compounds 1, 8, 10, 12, 14, 18, 19, 20, 21, 24, and 25 were assigned to be procyanidin dimmer, isorhamnetin-3-pyranosearabinose-7-glucosyl-rhamnoside, kaempferol-3-O-furananose-7-O-rhamnosyl-glucoside, rutin, isoquercetin, kaempferol-3-O-rutone, astragalin, quercetin, vitexin, kaempferol, and isorhamnetin [18], and compound 17 was assigned to be quercitrin [21]. Compounds 11 and 21 were assigned to be vitexin-rhamnoside [21] and vitexin [22]. Compound 21 was also detected in positive ion mode.…”

Validation of an optimized HPLC–UV method for the quantification of formononetin and biochanin A in Trifolium pratense extract

“…Determination of the chemical composition of medicinal plants has been a challenge because of the structural diversity of compounds contained by them [17]. Therefore, developing a standardized analytical technique is crucial for identifying compounds present in plant extracts [18].…”

“…For instance, formononetin is a very potent phytoestrogen and has many benefits in hormone therapy. It is well-documented to have anti-tumor, anti-proliferative, growth inhibitory, vasorelaxant, neuro-protective, anti-apoptotic, cardio-protective, mammary gland proliferative, anti-microbial, anti-inflammatory, and anti-oxidant activities [14,17,[19][20][21][22]. Biochanin A plays complex roles in controlling various biological functions by binding to DNA as well as several specific proteins.…”

“…It has been shown to have chemopreventive activity against various cancer cell lines and plays a therapeutic role in metabolic and hormonal disorders [24]. It also showed anti-inflammatory and neuroprotective effects [17,24].…”

Metabolic profile of alkaloids in Rhizoma Coptidis in rat plasma, urine and feces after oral administration using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

“…In recent years, it has been possible to identify metabolite peaks from a complex matrix by obtaining good chromatographic separation and accurate mass data with the improvement in resolution of chromatography and mass spectrometry . Ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC/QTOF‐MS) has been widely used in the field of analytical chemistry because of its high sensitivity and high resolution . In this work, an UPLC/QTOF‐MS method was established to identify the metabolites of the main active components in RC in rat plasma, urine and feces.…”