Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Abstract

Introduction The Paeonia ostii T. Hong & J. X. Zhang seed shell, characterised by a high content of oligostilbenes, is one of the two most important by‐products in the preparation of seed oil. Oligostilbenes are considered characteristic constituents of the genus Paeonia, and can be used in fingerprinting to determine the geographical origin and the quality of raw materials. Objective To develop and optimise a simple and reproducible high‐performance liquid chromatography diode array detection (HPLC‐DAD) method for the simultaneous determination of seven oligostilbenes in P. ostii seed shell from different geographical areas, and to associate the cultivation area. Methodology A validated HPLC method coupled with a DAD detector was performed for the detection and determination of target compounds in the samples. Optimal chromatographic conditions were achieved using an Agilent Zorbax Eclipse SB‐AQ‐C18 column and a gradient elution with acetonitrile and potassium dihydrogen phosphate solution. Results The proposed quantitative method showed appropriate accuracy and precision, and was successfully applied to the routine analysis of seven oligostilbenes and the quality evaluation of 50 P. ostii seed shell samples. There were significant differences between the contents of the seven oligostilbenes in different samples (P < 0.01). Conclusion The results demonstrated that the oligostilbenes were main secondary metabolites in the P. ostii seed shells, and the content of seven components in P. ostii seed shells sourced from different cultivation areas in China was different.

Ultrasonic-assisted extraction and adsorption separation: Large-scale preparation of trans-ε-Viniferin, suffruficosol B and trans-Gnetin H for the first time

“…It was found that oligostilbenes existed in the seeds, mainly concentrated in the seeds shell of species of genus Paeonia [3] , [4] , [5] , [6] . The research of our research group found that the seeds shell of oil tree peony contained a variety of oligostilbenes, and these compounds were presented at high levels in the seeds shell of oil tree peony.…”

“…The research of our research group found that the seeds shell of oil tree peony contained a variety of oligostilbenes, and these compounds were presented at high levels in the seeds shell of oil tree peony. Among them, SB, TV and TG had the highest content with important development and utilization value [3] , [7] , [8] . The bioactivities evaluation revealed that SB, TV and TG had good antioxidant [5] , antibacterial [9] , anti-inflammatory [10] , [11] , anti-tumor [4] , [9] , [12] , cognitive function improvement [13] , hypoglycemic effects [4] , enzyme inventory activity [4] , etc.…”

“…As a kind of authorized edible oil, tree peony seed oil (TPSO) was widely concerned for its rich content of unsaturated fatty acids, which led to the increasing cultivation area of oil tree peony year by year [14] , [15] . As a principal byproduct in TPSO production process, PSS occupied over 30 % of the total mass of oil tree peony seeds [3] , [8] , [9] , [15] . Therefore, the study on the extraction, enrichment and separation of oligostilbenes from PSS has broad development and application space.…”

“…The molecular structures of oligostilbenes in PSS possessed more phenolic hydroxyl groups, which resulted in the relative instability of oligostilbenes [3] , [5] , [9] . UAE was an effective method to extract oligostilbenes for the low extraction temperature, short extraction time and high efficiency [7] , [9] , [16] , [17] .…”

Phenolic Profile and Antioxidant Activity of the Edible Tree Peony Flower and Underlying Mechanisms of Preventive Effect on H2O2-Induced Oxidative Damage in Caco-2 Cells

“…Various phytochemicals, including phenolics [11,12], stilbenes [13], and terpenoids [14,15,16], were identified in tree peony in the previous studies. The phytochemical species and contents in tree peony differ according to the cultivars and organs involved [6,17,18,19].…”