Simultaneous determination of dihydroxybenzene positional isomers by capillary electrochromatography using gold nanoparticles as stationary phase

Abstract

The paper describes the enhanced separation of o-, m-, p-dihydroxybenzene by capillary electrochromatography (CEC) using gold nanoparticles (AuNPs) as stationary phase. The effect of the AuNPs concentration upon separation was investigated. The experimental parameters, including separation voltage, pH, and concentration of running buffer, were optimized. Under the optimum conditions, a good resolution of three dihydroxybenzene isomers was obtained within 15 min in a 50 cm effective length capillary modified with 0.02 nmol/L AuNPs at a separation voltage of 16 kV in a 50 mmol/L acetate buffer (pH 5.0). The linear ranges were from 10(-6) to 10(-4) mol/L and the detection limits were as low as 10(-7) mol/L. This method was successfully used to analysis two kinds of hair coloring agent sample with recoveries in the range of 90-105% and relative standard deviations (RSD) less than 5.0%.

Gold nanoparticle decorated graphene oxide/silica composite stationary phase for high-performance liquid chromatography

“…A). It had been reported that using modified GNPs for enantioselective recognition of chiral analytes, such as dansyl‐ d and l ‐norvaline, and in the work of He , GNPs were used as a stationary phase for separation of dihydroxybenzene positional isomers by CEC. All these above reports illustrated GNPs may possess some structural recognition capability.…”

“…As a pseudo‐stationary phase or stabilized on modified fused‐silica capillary, GNPs can separate biomolecules , neutral steroid drugs , and polycyclic aromatic hydrocarbons . GNPs can also be used as novel stationary phases providing high separation efficiencies for a variety of analytes in various separation systems , including GC and HPLC .…”

Recent advances in nanomaterial‐based capillary electrophoresis

“…An enzyme–antibody dual labeled AuNPs enhancing chemiluminescence (CL) strategy was developed for highly sensitive CE‐CL‐immunoassay of prostate‐specific antigen by Zhao's research group. They reported that the use of horseradish peroxidedase and antiprostate‐specific antigen–antibody labelled AuNPs complex as a marker can significantly enhance the CL signals, thus this method offers many advantages including high separation efficiency, simple operation, and low sample consumption .…”

Recent applications of nanomaterials in capillary electrophoresis

“…As already mentioned, the use of new nanomaterials also offers higher selectivity, even more as a result of the possibility of post‐attaching a large number of functionalities in the SP containing NPs (see Table ). In the case of AuNPs, although they have also been directly used as bare NPs , it must be taken into account their facility to be functionalized. As an example, You et al.…”