Simultaneous determination of antihistamine anti-allergic drugs, cetirizine, domperidone, chlorphenamine maleate, loratadine, meclizine and buclizine in pharmaceutical formulations, human serum and pharmacokinetics application

Perveen³

et al. 2019

Braz. J. Pharm. Sci.

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A novel reverse phase, isocratic HPLC method is described to separate five anti-diabetic drugs i.e., glimepiride, metformin, sitagliptin, rosiglitazone and pioglitazone. Nucleosil C 18 analytical column was used as stationary phase, while mobile phase consisted of acetonitrile:phosphate buffer: methanol (40/40/20, v/v) pH 2.0. Effluent was monitored at a flow rate 1 mL/min and detected at wavelength of 240 nm. This research produced excellent chromatography over a wide concentration range of 25-10000 ng/mL. Sepprated and well resolved quantifiable peaks were obtained and test results were linear in this range. Correlation coefficient of more than 0.9990 was witnessed as well as Low %RSD values i.e., maximum 2.0% documented excellent precision of the method. Good recoveries from pharmaecutical (99-101%), urine and plasma samples (>96%) in a range of concentrtion granted very good linearity, accuracy and precision. The projected method has satisfactory applications in quality control of these molecules as well as quantification of these molecules in urine and plasma samples.

Section: Specificity and Robustnessmentioning

confidence: 99%

Simultaneous determination of anti-diabetic drugs

Perveen³

et al. 2019

Braz. J. Pharm. Sci.

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“…Samples were prepared in multiple and passed through 0.45 µm membrane filter, before chromatography. [17][18][19][20]…”

Section: Serum Analysismentioning

confidence: 99%

Liquid Chromatographic Determination of Dexamethasone and Fluoroquinolones; in vitro Study

Perveen³

et al. 2019

S.Afr.j.chem.

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A precise, accurate and sensitive liquid chromatographic method is presented for the simultaneous analysis of moxifloxacin, levofloxacin, ciprofloxacin and dexamethasone in reference drugs, ophthalmic dosage form and in physiological fluids. Separation was achieved on a C 18 analytical column with acetonitrile-phosphate buffer (35:65 v/v) as mobile phase which was pumped isocratically at a flow rate of 1.0 mL min-1 and monitored at 254 nm wavelength. The calibration curve was linear in range of 40-10 000 ng mL-1. Test results in pharmaceutical and physiological samples showed excellent drug recovery (96.5-102.3 %) with good precision (0.81-2.3 % RSD). Test results were linear to sample concentration and correlation coefficient was more than 0.9990. The small value of quantification and detection levels showed the method to be sensitive enough for use in clinical and industrial investigations of all the mentioned drugs.

“…Like other antihistamines, HZ is reversibly blocking histamine H1 receptors, and thus expresses its physiological functions [4] . Cytochrome P450-2D6 in liver is known to be involved in its metabolism which oxidizes the alcohol moiety of the parent molecule [5] and thus produces a highly important carboxylated metabolite cetirizine (CZ) [6] .…”

Section: Introductionmentioning

confidence: 99%

New Method Development for Hydroxyzine Determination: Application in Stability Studies, Pharmaceutical Formulations, and Humane Serum

Siddiqui

Journal of Liquid Chromatography & Related Technologies

et al. 2015

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This article pertains to development and validation of an economically fast, sensitive and accurate Rp-HPLC method for quantitative analysis of HZ. A Hibar® μBondapak® C 18 column as stationary phase and acetonitrile: methanol: buffer (500:200:300) as mobile phase were used to accomplish the separation, when drawn at a flow rate of 1.0 mL/min, with 235 nm as monitoring wavelength. Linearity was established by studying the drug over the concentration range of 10-10000 ngmL -1 , correlation coefficient of r = 0.9993, drug recovery (97 to 102%) and high reproducibility in serum samples (less than 2.5% R.S.D) displayed excellent linearity, accuracy and precision. Force degradation studies of the drug under various stress conditions (acid, base, oxidation, photo and thermal) proved the stability indicating power of the method. Substantial method validation study was carried out inline with ICH guidelines and was applied successfully to quantify the amount of HZ in bulk, pharmaceutical formulations and blood serum samples. Downloaded by [Erciyes University]