Liquid Chromatographic Determination of Dexamethasone and Fluoroquinolones; in vitro Study

Abstract

A precise, accurate and sensitive liquid chromatographic method is presented for the simultaneous analysis of moxifloxacin, levofloxacin, ciprofloxacin and dexamethasone in reference drugs, ophthalmic dosage form and in physiological fluids. Separation was achieved on a C 18 analytical column with acetonitrile-phosphate buffer (35:65 v/v) as mobile phase which was pumped isocratically at a flow rate of 1.0 mL min-1 and monitored at 254 nm wavelength. The calibration curve was linear in range of 40-10 000 ng mL-1. Test results in pharmaceutical and physiological samples showed excellent drug recovery (96.5-102.3 %) with good precision (0.81-2.3 % RSD). Test results were linear to sample concentration and correlation coefficient was more than 0.9990. The small value of quantification and detection levels showed the method to be sensitive enough for use in clinical and industrial investigations of all the mentioned drugs.

An ion‐paired moxifloxacin nanosuspension eye drop provides improved prevention and treatment of ocular infection

“…MOX was analyzed by HPLC using a previously reported method with minor modifications 48,49 . The HPLC apparatus consisted of a binary pump (Shimadzu, Burnsville, MN), a Shimadzu SPD‐20A UV–Vis detector (Shimadzu), and a prominence autosampler injector.…”

“…MOX was analyzed by HPLC using a previously reported method with minor modifications. 48 , 49 The HPLC apparatus consisted of a binary pump (Shimadzu, Burnsville, MN), a Shimadzu SPD‐20A UV–Vis detector (Shimadzu), and a prominence autosampler injector. Briefly, a stock solution of MOX was prepared in water and/or methanol, and the stock solution was further diluted with acetonitrile to obtain a concentration range of 1–10 μg/ml.…”

“…MOX was analyzed by HPLC using a previously reported method with minor modifications. 48,49 The HPLC apparatus consisted of a binary MOX was monitored at 296 nm, and the retention time was 2.75 min.…”

A Novel RP-HPLC method for simultaneous estimation of Dorzolamide and Timolol maleate in ophthalmic products

“…A comprehensive method validation protocol was used to establish method validation. It included linearity, range, specificity, accuracy, precision, robustness, and system suitability ICH, [16][17][18].…”

Stepwise optimization and sensitivity improvement of green micellar electrokinetic chromatography method to simultaneously determine some fluoroquinolones and glucocorticoids present in various binary ophthalmic formulations

“…Various methods have been reported describing the assay of the fluoroquinolones and corticosteroids either singly or in combination. These methods include spectrophotometry (Abdel‐Razeq, Darwish, Zaazaa, Nasr, & Zeinab, 2015; Patel & Patel, 2013; Pradhan, Raiyani, Shah, Patel, & Upadhyay, 2015; Sversut et al, 2017), HPLC (El Gammal, El‐Wasseef, El‐Ashry, & Saadia, 2018; Gandhi, Rao, & Rao, 2016; Saad, Essam, Elzanfaly, & Amer, 2020; Sher, Fatima, Perveen, & Siddiqui, 2019; Sversut et al, 2014), thin‐layer chromatography (TLC) (Dorofeev, Konovalov, Kochin, & Arzamastsev, 2004; Musharraf, Fatima, & Sultana, 2012; Saad et al, 2020; Seid, Hymete, & Bekhit, 2012), capillary electrophoresis (Al Azzam, Saad, Adnan, & Aboul‐Enein, 2010; Alnajjar, 2013; Bourdon et al, 2013; Gallego & Arroyo, 2003; Horstkötter & Blaschke, 2001; Maher et al, 2013; Olędzka, Kowalski, Plenis, & Bączek, 2017; British Pharmacopoeia, 2013; Song, Bai, Jia, & Zhou, 2008; Suliman, Elbashir, & Schmitz, 2015; Wang et al, 2009; Zhu, Jiang, & Hu, 2002) and electrochemical methods (El‐Rahman, Lotfy, Hegazy, Rezk, & Rostom, 2015; Smajdor, Piech, & Paczosa‐Bator, 2016; Zhang, Gu, Ding, Li, & Liu, 2013; Zhang, Gu, Ding, Zhang, & Li, 2013). After a thorough investigation of literature, it was observed that no capillary electrophoretic method has been published for the simultaneous assay of the four chosen drugs.…”