Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography–electrospray mass spectrometry

Bonato, Pierina Sueli; Oliveira, Anderson Rodrigo Moraes de; Santana, Fernando José Malagueño de; Fernandes, B. J.; Lanchote, Vera Lúcia; González, Armando E.; Garcı́a, Héctor H.; Takayanagui, Osvaldo Massaiti

doi:10.1016/j.jpba.2006.11.020

Cited by 39 publications

(21 citation statements)

References 32 publications

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“…Plasma samples were analysed by a previously validated analytical method using LC‐MS/MS [20]. The plasma samples (1 ml) spiked with phenacetin as internal standard were prepared by liquid–liquid extraction and ABSO and PZQ were separated on a Lichrospher CN column using methanol : water (3:7, v : v) plus 0.5% of acetic acid as mobile phase.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of combined treatment with praziquantel and albendazole in neurocysticercosis

Garcı́a

Lescano

Lanchote

et al. 2011

Brit J Clinical Pharma

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WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT• The antiparasitic drugs albendazole and prazoquantel have been used together before for geohelminths, echinococcosis and cysticercosis, but there is only scarce and discrepant data on the pharmacokinetics of these drugs when given together. WHAT THIS STUDY ADDS• This is a randomized, double-blinded, placebo-controlled phase II evaluation of the pharmacokinetics of albendazole and praziquantel in 32 patients with neurocysticercosis which demonstrates that combined albendazole + praziquantel is associated with increased albendazole sulfoxide concentrations. These increased concentrations could independently contribute to increased cysticidal efficacy by themselves or in addition to a possible synergistic effect. AIMSNeurocysticercosis is the most common cause of acquired epilepsy in the world. Antiparasitic treatment of viable brain cysts is of clinical benefit, but current antiparasitic regimes provide incomplete parasiticidal efficacy. Combined use of two antiparasitic drugs may improve clearance of brain parasites. Albendazole (ABZ) has been used together with praziquantel (PZQ) before for geohelminths, echinococcosis and cysticercosis, but their combined use is not yet formally recommended and only scarce, discrepant data exist on their pharmacokinetics when given together. We assessed the pharmacokinetics of their combined use for the treatment of neurocysticercosis. METHODSA randomized, double-blind, placebo-controlled phase II evaluation of the pharmacokinetics of ABZ and PZQ in 32 patients with neurocysticercosis was carried out. Patients received their usual concomitant medications including an antiepileptic drug, dexamethasone, and ranitidine. Randomization was stratified by antiepileptic drug (phenytoin or carbamazepine). Subjects had sequential blood samples taken after the first dose of antiparasitic drugs and again after 9 days of treatment, and were followed for 3 months after dosing. RESULTSTwenty-one men and 11 women, aged 16 to 55 (mean age 28) years were included. Albendazole sulfoxide concentrations were increased in the combination group compared with the ABZ alone group, both in patients taking phenytoin and patients taking carbamazepine. PZQ concentrations were also increased by the end of therapy. There were no significant side effects in this study group. CONCLUSIONSCombined ABZ + PZQ is associated with increased albendazole sulfoxide plasma concentrations. These increased concentrations could independently contribute to increased cysticidal efficacy by themselves or in addition to a possible synergistic effect.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of combined treatment with praziquantel and albendazole in neurocysticercosis

Garcı́a

Lescano

Lanchote

et al. 2011

Brit J Clinical Pharma

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“…Many publications describe the determination of ABZ and PZQ in pharmaceutical formulations. Individual and simultaneous determination with other drugs are possible by FIA using UV-detection and HPLC method in suspensions, 3 adsorptive stripping voltammetry (ASV) and linear sweep voltammetry (LSV), square-wave voltammetry (SWV), differential pulse voltammetry (DPV), 4,5 Vis-spectrophotometry, [6][7][8][9][10] HPLC, 11,12 derivative UV-spectrophotometry, 13,14 GLC 15 and PMR spectrometry. 16 In biological samples for individuals, The simultaneous determination of ABZ and PZQ has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Albendazole and Praziquantel by Second Derivative Spectrophotometry and Multivariated Calibration Methods in Veterinary Pharmaceutical Formulation

et al. 2010

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The simultaneous determination of albendazole (ABZ) and praziquantel (PZQ) was performed by different mathematical approaches: second derivative spectrophotometry (SDS), classical least squares, regression of partial least squares and principal components regression based on spectral data of drugs dissolved in methanol-hydrochloric acid solution. The detection limits for multivariate calibrations were determined by creating a surrogate variable signal. SDS presented the best analytical features. The recoveries of ABZ and PZQ from the synthetic samples were near to 100 ± 5%. The methods were applied in veterinary pharmaceutical formulation whose mass ratio ABZ:PZQ is 10:1; the results obtained were according to nominal content.

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“…The PZQ residues are usually extracted from plasma with an organic solvent like dichloromethane, toluene, acetonitrile and further clean-up is made by solid-phase extraction (SPE) with C 18 , silica or strong cation-exchange (SCX) cartridges [6][7][8][9]. Ethyl acetate was chosen as extraction solvent because it can be evaporated rapidly under vacuum at 45 • C. This evaporation step is critical.…”

Section: Methods Developmentmentioning

confidence: 99%

Simple, cheap and effective high-performance liquid chromatographic method for determination of praziquantel in bovine muscle

Sun

Shi-jin

2012

Journal of Chromatography B

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Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography–electrospray mass spectrometry

Cited by 39 publications

References 32 publications

Pharmacokinetics of combined treatment with praziquantel and albendazole in neurocysticercosis

Pharmacokinetics of combined treatment with praziquantel and albendazole in neurocysticercosis

Simultaneous Determination of Albendazole and Praziquantel by Second Derivative Spectrophotometry and Multivariated Calibration Methods in Veterinary Pharmaceutical Formulation

Simple, cheap and effective high-performance liquid chromatographic method for determination of praziquantel in bovine muscle

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