Simple, cheap and effective high-performance liquid chromatographic method for determination of praziquantel in bovine muscle

Sun, Yu; Shi-jin, BU

doi:10.1016/j.jchromb.2012.05.004

Cited by 17 publications

(8 citation statements)

References 7 publications

(10 reference statements)

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“…HPLC analysis was also used to calculate the solubility and the dissolution properties of the different samples. The method was adapted from literature [ 26 ] and have been already validated and employed for PZQ quantification in previous studies [ 11 , 12 , 13 ]. Briefly, the HPLC system consisted of two mobile phase delivery pumps (LC-10ADvp, Shimadzu, Japan) and a UV–vis detector (SPD-10Avp, Shimadzu, Japan).…”

Section: Methodsmentioning

confidence: 99%

Combining Mechanochemistry and Spray Congealing for New Praziquantel Pediatric Formulations in Schistosomiasis Treatment

Albertini

Perissutti

Bertoni

et al. 2019

IJMS

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Praziquantel (PZQ) is the first line drug for the treatment of schistosome infections and is included in the WHO Model List of Essential Medicines for Children. In this study, the association of mechanochemical activation (MA) and the spray congealing (SC) technology was evaluated for developing a child-friendly PZQ dosage form, with better product handling and biopharmaceutical properties, compared to MA materials. A 1:1 by wt PZQ—Povidone coground—was prepared in a vibrational mill under cryogenic conditions, for favoring amorphization. PZQ was neat ground to obtain its polymorphic form (Form B), which has an improved solubility and bioactivity. Then, activated PZQ powders were loaded into microparticles (MPs) by the SC technology, using the self-emulsifying agent Gelucire® 50/13 as a carrier. Both, the activated powders and the corresponding loaded MPs were characterized for morphology, wettability, solubility, dissolution behavior, drug content, and drug solid state (Hot Stage Microscopy (HSM), Differential Scanning Calorimetry (DSC), X-Ray Powder Diffraction Studies (PXRD), and FT-IR). Samples were also in vitro tested for a comparison with PZQ against Schistosoma mansoni newly transformed schistosomula (NTS) and adults. MPs containing both MA systems showed a further increase of biopharmaceutical properties, compared to the milled powders, while maintaining PZQ bioactivity. MPs containing PZQ Form B represented the most promising product for designing a new PZQ formulation.

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Section: Methodsmentioning

confidence: 99%

Combining Mechanochemistry and Spray Congealing for New Praziquantel Pediatric Formulations in Schistosomiasis Treatment

Albertini

Perissutti

Bertoni

et al. 2019

IJMS

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“…The content of PZQ after milling was assayed by means of a reverse phase HPLC-UV method [24], reported in literature and already applied in previous experiments [17][18][19].…”

Section: Physical Characterization Of the Physical Mixtures And Coground Samples And Drug Recoverymentioning

confidence: 99%

Impact of mesoporous silica on the chemical degradation of Praziquantel upon grinding

Šagud¹,

Zanolla²,

Zingone³

et al. 2021

Comptes Rendus. Chimie

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Praziquantel and Syloid 244 FP (amorphous silicon dioxide) were ground in a vibrational mill in standard conditions (15 or 30 at 25 Hz). Amorphous solid dispersions were obtained in a very short grinding time (15 min), as testified by DSC, PXRD and ESEM analyses. Samples ground at 15 and 30 showed the presence of the same degradation product, as was evident from UPLC, MS and NMR analyses. This short paper brings to light the tremendous lattice de-structuration ability of Syloid 244 FP upon grinding toward Praziquantel as well as its detrimental influence on promoting chemical degradation through ring opening and oxidation.

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“…After optimization (1-7) represents concentrations of 0, 0.1, 0.25, 0.5, 1, 2.5, and 5 ng/mL. 2017); in bovine muscle using HPLC with a quantification limit of 0.02 mg/kg and a detection limit of 0.01 mg/kg (Sun & Bu, 2012), and in edible bovine tissues using liquid chromatography-tandem mass spectrometry with an LOD of 1 mug/kg and at a minimum limit of quantification of 2.5 mug/kg. In conclusion, the chromatographic assays remain the most common high sensitivity assay for the determination of analytes.…”

Section: Determination Of Pzq In Lateolabrax Japonicusmentioning

confidence: 99%

Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay

Hao

Suryoprabowo

Kuang

et al. 2020

Food and Agricultural Immunology

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Praziquantel (PZQ) is a compound that usually used as insecticide, drug residue could be harm to human health. In this study, we have developed a simple, sensitive, and rapid lateral flow immunochromatographic assay (LFIA) for the detection of PZQ in fish (Lateolabrax Japonicus). We produced a sensitive mAb using enzyme-linked immunosorbent assay with half-maximal inhibitory concentration (2A11, IC50) of 2.06 ng/mL. The coating antigen (PZQ-HS-GA-OVA) and goat anti-mouse IgG antibody were sprayed onto a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cutoff limits for PZQ detection on the test strips were determined to be 5 ng/mL in both 0.01 M phosphate buffered saline and fish samples, and results were obtained within 5 min. Our data suggests that this LFIA can be used for the sensitive, rapid, and specific on-site screening for PZQ in fish.

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Simple, cheap and effective high-performance liquid chromatographic method for determination of praziquantel in bovine muscle

Cited by 17 publications

References 7 publications

Combining Mechanochemistry and Spray Congealing for New Praziquantel Pediatric Formulations in Schistosomiasis Treatment

Combining Mechanochemistry and Spray Congealing for New Praziquantel Pediatric Formulations in Schistosomiasis Treatment

Impact of mesoporous silica on the chemical degradation of Praziquantel upon grinding

Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay

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