Simultaneous detection of gastric cancer-involved miR-106a and let-7a through a dual-signal-marked electrochemical nanobiosensor

Abstract

miRNAs are among the novel biomarkers that can be evaluated for sensitive and early cancer diagnosis. In the present study, an electrochemical nanobiosensor has been fabricated to detect two gastric cancer (GC) related miRNAs simultaneously. By employing Au nanoparticles- and CdSe@CdS quantum dots-contained magnetic nanocomposites as electrochemical labels along with the polythiophene/reduced graphene oxide-modified carbon electrodes, this dual signal nanobiosensor showed a considerable performance in quantifying miR-106a (a GC oncogenic miRNA) and let-7a (a GC tumor suppressor miRNA). Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), not only the accomplishment of desired biosensing platform construction was confirmed, but also its great specificity, appropriate selectivity, acceptable stability, and significant sensitivity were indicated. Through the combined analyses of the dual miRNAs, our nanobiosensor reached detection limit of 0.02 fM and 0.06 fM for let-7a and miR-106a, respectively. This multiplex PCR-free miRNA nanobiosensor demonstrates attractive potentials for promising applications in early diagnosis of GC and additionally the screen of any miRNA sequence.

Microbial Fabricated Nanosystems: Applications in Drug Delivery and Targeting

“…They were also found compatible with red blood cells with no observed hemolysis implying their potential biomedical applications (Fahmy et al, 2020). Cadmium sulfide (CdS) nanocrystals were synthesized by the non-sulfur bacterium Rhodopseudomonas palustris growing on cadmium sulfate (CdSO 4 ) (Bai et al, 2009) which were used in the design of biosensing devices for molecular diagnosis (Daneshpour et al, 2018). A cell-free supernatant of actinobacteria was used to synthesize Fe-NP revealing its strong antioxidant and antimicrobial activity.…”

Point-of-care biosensors in medicine: a brief overview of our achievements in this field based on the conducted research in EMRI (endocrinology and metabolism research Institute of Tehran University of medical sciences) over the past fourteen years

“…In 2018, this group can develop an electrochemical genosensor to recognize two cancer related miRNAs simultaneously by using Au nanoparticles and CdSe@CdS quantum dots-contained magnetic nanocomposite as tracing tags along with the polythiophene/reduced graphene oxide-modified electrodes. The presented POC system showed the detection limit around 0.02 fM and 0.06 fM for let-7a and miR-106a, respectively [ 14 ].…”

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

“…Since the year 2,000, miRNAs have been widely studied worldwide and more than two thousand miRNAs have been identified and characterized. miRNAs are widely involved in the development of gastric cancer ( Daneshpour et al, 2018 ; Huang et al, 2019 ). H. pylori (HP) is a class I gastric carcinogen.…”