Simultaneous Analysis of Seven Biomarkers of Oxidative Damage to Lipids, Proteins, and DNA in Urine

Martinez, Maria P.; Kannan, Kurunthachalam

doi:10.1021/acs.est.8b00883

Cited by 42 publications

(30 citation statements)

References 31 publications

(135 reference statements)

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“…In many structural proteins, dityrosine confers resistance to proteolysis and physicochemical trauma as a stabilizing crosslink (DiMarco and Giulivi, 2007). Dityrosine has also been found in oxidative/nitrative stress under a variety of conditions and biological systems because tyrosine is sensitive to hydroxyl radical and is easily oxidized into its dimer form (Martinez and Kannan, 2018). Furthermore, oxidized tyrosine and dityrosine exposure changed systemic metabolic diseases and object recognition deficits in rodents (Ran et al, 2016; Yang et al, 2017b).…”

Section: Resultsmentioning

confidence: 99%

Tetramethylpyrazine Protects Oxidative Stability and Gelation Property of Rabbit Myofibrillar Proteins

Wang

Liu

Zhang

2019

Food Sci Anim Resour

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Tetramethylpyrazine (TMP), an alkaloid rich in Ligusticum wallichii and fermented products, possesses multiple pharmacological activities in antioxidant, anti-inflammatory, and antibacterial. This study aimed to investigate the effect of TMP (15 mg/L) on the physicochemical and gelation properties of rabbit myofibrillar proteins (MPs) with/without oxidative stress. Results showed that compared to the control, oxidative stress to MPs decreased free thiol content, gel yield, whiteness, water-holding capacity, bounder water, immobilized water, and endogenous tryptophan fluorescence intensity, but increased surface hydrophobicity, dityrosine content, and free water content (p<0.01). Without oxidative stress, MPs treated with TMP increased free thiol content, whiteness, and bound water, but decreased dityrosine content and free water (p<0.05). Under oxidative conditions, all parameters were conversely affected by TMP (p<0.01). The results suggest that TMP can be an antioxidant to decrease the concern on oxidative deterioration during meat processing and storage by improving the oxidative stability, water retention, and gel forming property of rabbit MPs.

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Section: Resultsmentioning

confidence: 99%

Tetramethylpyrazine Protects Oxidative Stability and Gelation Property of Rabbit Myofibrillar Proteins

Wang

Liu

Zhang

2019

Food Sci Anim Resour

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“…Institutional Review Board approvals of the New York State Department of Health were obtained for the analysis of OSBs in urine. Creatinine, o , o ′-dityrosine (diY), 8-hydroxy-2′-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and four F 2 –isoprostane isomers (8-PGF 2α , 11-PGF 2α , 15-PGF 2α , and 8,15-PGF 2α ) were determined in urine from the same set of samples, as reported previously …”

Section: Methodsmentioning

confidence: 98%

Allantoin as a Marker of Oxidative Stress: Inter- and Intraindividual Variability in Urinary Concentrations in Healthy Individuals

Martinez-Moral

2019

Environ. Sci. Technol. Lett.

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Altered systemic oxidative stress is a consequence of environmental exposures and diseases, among other stress factors. The extent of oxidative stress in humans is assessed through the analysis of oxidative stress biomarkers (OSBs) in biospecimens such as urine. Allantoin is an OSB, a specific product of the oxidation of uric acid, that is measurable in urine. In this study, concentrations and inter-and intraindividual variability in urinary concentrations of allantoin were determined in healthy individuals. The results were compared with those found for other OSBs of lipids, proteins, and DNA in the same set of samples. Allantoin was found in all urine samples (N = 515), at concentrations that ranged from 0.88 to 41.7 mmol mol −1 creatinine (0.90 to 112 μg mL −1 urine). The intraclass correlation coefficient (ICC) of urinary allantoin measured in samples collected longitudinally for over a month from 19 individuals (0.74 for creatinine corrected and 0.80 for volumetric concentrations) showed an excellent reliability and notable interindividual variability. Among eight biomarkers measured in the same set of urine samples, allantoin was the predominant one, and it was positively and significantly correlated with urinary creatinine and other OSBs of lipids, proteins, and DNA. We suggest that urinary allantoin is a suitable biomarker of oxidative stress because of its requirement for simple and rapid analysis and long-term reliability/reproducibility as determined by high ICC values.

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“…Depending on the analytes of interest, sometimes SPE is used together with another sample preparation technique in order to ensure that every analyte is detected. In 2018, Martinez and Kannan developed and validated an LC–MS/MS method for the simultaneous determination of seven different urinary biomarkers of oxidative damage to lipids, proteins, and DNA, using SPE preceded by a derivatization step with 2,4‐dinitrophenylhydrazine [122]. Despite the fact that several studies have already determined these biomarkers together, this research is innovative as the previous studies all used multiple analytical techniques [123–125].…”

Section: Strategies To Quantify Biomarkers In Human Urine (2015–2020)mentioning

confidence: 99%

Recent progress in the LC–MS/MS analysis of oxidative stress biomarkers

et al. 2020

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The presence of a dynamic and balanced equilibrium between the production of reactive oxygen (ROS) and nitrogen (RNS) species and the in‐house antioxidant defense mechanisms is characteristic for a healthy body. During oxidative stress (OS), this balance is switched to increased production of ROS and RNS, exceeding the capacity of physiological antioxidant systems. This can cause damage to biological molecules, leading to loss of function and even cell death. Nowadays, there is increasing scientific and clinical interest in OS and the associated parameters to measure the degree of OS in biofluids. An increasing number of reports using LC–MS/MS methods for the analysis of OS biomarkers can be found. Since bioanalysis is usually complicated by matrix effects, various types of cleanup procedures are used to effectively separate the biomarkers from the matrix. This is an essential part of the analysis to prepare a reproducible and homogenous solution suitable for injection onto the column. The present review gives a summary of the chromatographic methods used for the determination of OS biomarkers in both urine and plasma, serum, and whole blood samples. The first part mainly describes the biological background of the different OS biomarkers, while the second part reports examples of chromatographic methods for the analysis of different metabolites connected with OS in biofluids, covering a period from 2015 till early 2020. The selected examples mainly include LC–MS/MS methods for isoprostanes, oxidized proteins, oxidized lipoproteins, and DNA/RNA biomarkers. The last part explains the clinical relevance of this review.

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Simultaneous Analysis of Seven Biomarkers of Oxidative Damage to Lipids, Proteins, and DNA in Urine

Cited by 42 publications

References 31 publications

Tetramethylpyrazine Protects Oxidative Stability and Gelation Property of Rabbit Myofibrillar Proteins

Tetramethylpyrazine Protects Oxidative Stability and Gelation Property of Rabbit Myofibrillar Proteins

Allantoin as a Marker of Oxidative Stress: Inter- and Intraindividual Variability in Urinary Concentrations in Healthy Individuals

Recent progress in the LC–MS/MS analysis of oxidative stress biomarkers

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