Simulated synovial fluids for in vitro drug and prodrug release testing of depot injectables intended for joint injection

Thing, Mette; Mertz, Nina; Ågårdh, Li; Larsen, Susan Weng; Østergaard, Jesper; Larsen, Claus

doi:10.1016/j.jddst.2018.11.012

Cited by 12 publications

(5 citation statements)

References 54 publications

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“…Disadvantages are related to the limitation in the obtainment of adequate amounts from patients [18]. One alternative can be the use of bovine, porcine or equine SF [19] or the use of simulated SF [20,21].…”

Section: Introductionmentioning

confidence: 99%

Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

et al. 2021

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Bacterial antibiotic resistance and biofilm formation are mechanisms usually involved in the pathogeny of implant-related infections. Worldwide, antibiotic susceptibility tests are usually carried out using nutrient-rich media. Clinical routine laboratories and even research centers use for example EUCAST or CLSI for guidelines. In this study, we investigated the effect of different nutrient media on the antibiotic susceptibility and icaADBC gene expression of bacteria in biofilm. As media, Müller-Hinton Bouillon (MHB), Tryptic Soy Broth (TSB) and human synovial fluid (SF) diluted 1:4 in phosphate buffered saline (PBS), each also supplemented with 1% glucose, were used. The influence of different nutrient media on the antibiotic susceptibility of coagulase-negative staphylococci (CoNS) was evaluated by counting of colony-forming units (CFU) and by checking the metabolic activity of the bacteria. We used reverse transcriptase and real-time qPCR to investigate the influence of nutrient media on the biofilm gene expression. We used two-way analysis of variance (ANOVA). p < 0.05 was considered to be statistically significant. Significant differences in growth and antibiotic susceptibility were detected in all strains tested among the different media used. The nutrient media showed influence on the cell viability of all bacteria after antibiotic treatment. IcaADBC gene expression was significantly influenced by glucose and all nutrient media. The results highlight the influence of glucose on the antibiotic susceptibility, growth and gene expression of all strains tested. For all strains, a significant difference in bacterial recovery, viability and gene expression were found when compared to biofilm grown in SF.

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Section: Introductionmentioning

confidence: 99%

Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

et al. 2021

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“…2 ). In human SF, proteins with MW > 160 kDa are totally absent [ 41 ]; subsequently, the cut-off of a healthy synovial membrane is lower than 160 kDa. Samples of the conjugate HA-ALD and the physical mixture HA + ALD were placed in a 20 kDa dialysis and total released phosphorous was measured in the reservoir at different time points.…”

Section: Resultsmentioning

confidence: 99%

Hyaluronic acid-alendronate conjugate: A macromolecular drug delivery system for intra-articular treatment of osteoarthritis

Pluda

Beninatto

Soato

et al. 2021

Osteoarthritis and Cartilage Open

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“…Apart from the MMPs, esterase is detected in the synovial fluid of OA patients. [75][76][77] Since the ester bonds present in TG-18 are susceptible to esterase and MMPs, we used it to achieve sustained release of BI-4394. 51 To investigate the release of the encapsulated BI-4394 in response to the enzymes, Gel@5 and Gel@10 were placed in dialysis bags containing 100 U mL À1 esterase.…”

Section: Rheologymentioning

confidence: 99%

On-demand release of a selective MMP-13 blocker from an enzyme-responsive injectable hydrogel protects cartilage from degenerative progression in osteoarthritis

Roy,

Murugesan,

Kulkarni

et al. 2024

J. Mater. Chem. B

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show abstract

Simulated synovial fluids for in vitro drug and prodrug release testing of depot injectables intended for joint injection

Cited by 12 publications

References 54 publications

Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

Hyaluronic acid-alendronate conjugate: A macromolecular drug delivery system for intra-articular treatment of osteoarthritis

On-demand release of a selective MMP-13 blocker from an enzyme-responsive injectable hydrogel protects cartilage from degenerative progression in osteoarthritis

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