Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

Steixner, Stephan; Spiegel, Christopher; Dammerer, Dietmar; Wurm, Alexander; Nogler, Michaël; Coraça‐Huber, Débora C.

doi:10.3390/antibiotics10070790

Cited by 6 publications

(8 citation statements)

References 49 publications

(48 reference statements)

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“…A possible cause of the higher results in hemoglobin medium may be the lower concentration of antibiotics. 20 , 21 According to the CLSI M100 Performance Standards, cysteine-containing defined growth supplement will significantly alter dilution test results with carbapenems and clavulanate. Hence, the role of the specific components in different growth supplements by the control variable method will be our next focus.…”

Section: Discussionmentioning

confidence: 99%

Impact of Alternative Growth Supplements on Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae

Zhou¹,

Xu²,

Xia³

et al. 2022

IDR

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Purpose The accurate detection of antibiotic susceptibility of Neisseria gonorrhoeae ( N. gonorrhoeae ) is of great importance for the treatment of patients with gonorrhea as well as to hinder the progress of drug resistance. To promote the application of gonococcal antibiotic susceptibility monitoring in primary hospitals and remote medical institutions, this study evaluated the effect of alternative growth supplements on the antibiotic susceptibility testing of N. gonorrhoeae isolates. Methods We divided the antimicrobial-containing media into three groups by adding different growth supplements (sterile defibrinated sheep blood, bovine hemoglobin, and Vitox). We tested the antimicrobial susceptibility of 80 N. gonorrhoeae isolates in different groups against eight antibiotics. Nonparametric signed-rank tests were utilized to compare the minimum inhibitory concentration (MIC) results of each group. Taking the MIC results of Vitox group as expected, the essential agreement (EA) and category agreement (CA) of the other two groups were calculated. Results For the group using sheep blood as growth supplements, the EA values and CA values of each antibiotic were above 90.00% and minor error rates were less than 7.00%. No very major error and major error were observed. For the group using hemoglobin as growth supplements, the EA values of the susceptibility results of zoliflodacin, penicillin, and ceftriaxone were lower than 90.00%. The overall MIC results of using hemoglobin as a growth supplement were higher than those of sheep blood and Vitox in the susceptibility testing of these three antibiotics. Conclusion Compared with the expected results, sheep blood may be considered for the use as an alternative material for N. gonorrhoeae antibiotics susceptibility surveillance, while hemoglobin may not be suitable for supplement to antimicrobial-containing medium.

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Section: Discussionmentioning

confidence: 99%

Impact of Alternative Growth Supplements on Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae

Zhou¹,

Xu²,

Xia³

et al. 2022

IDR

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“…Staphylococcus epidermidis ( S. epidermidis ) is one of the most common Coagulase-Negative Staphylococci (CoNS) [ 1 ]. The average person carries 10 to 24 strains of this bacteria on the surface of the skin and mucous membranes.…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Silver Nanoparticles in Changing the Biofilm-Related Gene Expression of Staphylococcus epidermidis

Swolana

Kępa

Kruszniewska‐Rajs

et al. 2022

IJMS

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Nowadays, antibiotic resistance is a major public health problem. Among staphylococci, infections caused by Staphylococcus epidermidis (S. epidermidis) are frequent and difficult to eradicate. This is due to its ability to form biofilm. Among the antibiotic substances, nanosilver is of particular interest. Based on this information, we decided to investigate the effect of nanosilver on the viability, biofilm formation and gene expression of the icaADBC operon and the icaR gene for biofilm and non-biofilm S. epidermidis strains. As we observed, the viability of all the tested strains decreased with the use of nanosilver at a concentration of 5 µg/mL. The ability to form biofilm also decreased with the use of nanosilver at a concentration of 3 µg/mL. Genetic expression of the icaADBC operon and the icaR gene varied depending on the ability of the strain to form biofilm. Low concentrations of nanosilver may cause increased biofilm production, however no such effect was observed with high concentrations. This confirms that the use of nanoparticles at an appropriately high dose in any future therapy is of utmost importance. Data from our publication confirm the antibacterial and antibiotic properties of nanosilver. This effect was observed phenotypically and also by levels of gene expression.

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“…Clearly, growth in TSB only is a very poor approximation to the in vivo environment, and thus, relevant mechanisms related to S. epidermidis pathogenesis may have gone unidentified by their use. Particularly for PJI, studies provided evidence that in vitro experiments in convenient laboratory media do not reflect bacterial growth in vivo under conditions of a human joint cavity (Skovdal et al, 2021;Steixner et al, 2021). Bacteria entering a human joint cavity have to adapt to the unique environment provided by the synovial joint fluid (SF), which lubricates the joint cavity.…”

Section: Introductionmentioning

confidence: 99%

“…Despite this fact, however, only a few studies have so far been conducted using unmodified human SF (Dastgheyb et al, 2015;Gilbertie et al, 2019). Typically, human SF is diluted before use (Knott et al, 2021;Staats et al, 2021;Steixner et al, 2021), or substituted by animal SF (Gupta et al, 2021;Perez and Patel, 2015;Ibberson et al, 2016;Pestrak et al, 2020;Staats et al, 2021). The obvious limitations of these approaches currently have to be accepted due to the limited availability of human SF (Gilbertie et al, 2019;Steixner et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Typically, human SF is diluted before use (Knott et al, 2021;Staats et al, 2021;Steixner et al, 2021), or substituted by animal SF (Gupta et al, 2021;Perez and Patel, 2015;Ibberson et al, 2016;Pestrak et al, 2020;Staats et al, 2021). The obvious limitations of these approaches currently have to be accepted due to the limited availability of human SF (Gilbertie et al, 2019;Steixner et al, 2021). While substitute joint fluid to treat joint disease has already been introduced into modern medicine (Adams et al, 1995;Neustadt et al, 2005;Faust et al, 2018), only a few attempts to use a bona fide artificial SF in pathogenesis research have been made (Knott et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Development of an artificial synovial fluid useful for studying Staphylococcus epidermidis joint infections

Stamm

Weißelberg²,

Both³

et al. 2022

Front. Cell. Infect. Microbiol.

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Staphylococcus epidermidis is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective pathogenic potential. In vitro studies essentially relying on phenotypic assays and genetic approaches have provided a detailed picture of the molecular events contributing to biofilm assembly. A major limitation in these studies is the use of synthetic growth media, which significantly differs from the environmental conditions S. epidermidis encounters during host invasion. Building on evidence showing that growth in serum substantially affects S. epidermidis gene expression profiles and phenotypes, the major aim of this study was to develop and characterize a growth medium mimicking synovial fluid, thereby facilitating research addressing specific aspects related to PJI. Using fresh human plasma, a protocol was established allowing for the large-scale production of a medium that by biochemical analysis matches key characteristics of synovial fluid and therefore is referred to as artificial synovial fluid (ASF). By analysis of biofilm-positive, polysaccharide intercellular adhesion (PIA)-producing S. epidermidis 1457 and its isogenic, PIA- and biofilm-negative mutant 1457-M10, evidence is provided that the presence of ASF induces cluster formation in S. epidermidis 1457 and mutant 1457-M10. Consistent with the aggregative properties, both strains formed multilayered biofilms when analyzed by confocal laser scanning microscopy. In parallel to the phenotypic findings, expression analysis after growth in ASF found upregulation of genes encoding for intercellular adhesins (icaA, aap, and embp) as well as atlE, encoding for the major cell wall autolysin being responsible for eDNA release. In contrast, growth in ASF was associated with reduced expression of the master regulator agr. Collectively, these results indicate that ASF induces expression profiles that are able to support intercellular adhesion in both PIA-positive and PIA-negative S. epidermidis. Given the observation that ASF overall induced biofilm formation in a collection of S. epidermidis isolates from PJI, the results strongly support the idea of using growth media mimicking host environments. ASF may play an important role in future studies related to the pathogenesis of S. epidermidis PJI.

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Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro

Cited by 6 publications

References 49 publications

Impact of Alternative Growth Supplements on Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae

Impact of Alternative Growth Supplements on Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae

Antibiofilm Effect of Silver Nanoparticles in Changing the Biofilm-Related Gene Expression of Staphylococcus epidermidis

Development of an artificial synovial fluid useful for studying Staphylococcus epidermidis joint infections

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