Simple Two-step Chromatographic Method for Purification of Ovomacroglobulin

Geng, Fang; Huang, Xi; Mei-Hu,; Li, Bingbing; Zhang, Xiaowei

doi:10.14233/ajchem.2013.13656

Cited by 4 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ovomacroglobulin consists of four subunits of equal molecular weight (SDS-PAGE analysis indicates approximately 175 kDa), two of which are linked by disulfide bonds. Various biological functions of ovomacroglobulin have been reported, including antibacterial and anti-inflammatory effects, keratitis treatment, and hemagglutination inhibition [ 104 ].…”

Section: Fractionation Of Egg Proteinsmentioning

confidence: 99%

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

et al. 2023

View full text Add to dashboard Cite

This review article discusses advanced extraction methods to enhance the functionality of egg-derived peptides while reducing their allergenicity. While eggs are considered a nutrient-dense food, some proteins can cause allergic reactions in susceptible individuals. Therefore, various methods have been developed to reduce the allergenicity of egg-derived proteins, such as enzymatic hydrolysis, heat treatment, and glycosylation. In addition to reducing allergenicity, advanced extraction methods can enhance the functionality of egg-derived peptides. Techniques such as membrane separation, chromatography, and electrodialysis can isolate and purify specific egg-derived peptides with desired functional properties, improving their bioactivity. Further, enzymatic hydrolysis can also break down polypeptide sequences and produce bioactive peptides with various health benefits. While liquid chromatography is the most commonly used method to obtain individual proteins for developing novel food products, several challenges are associated with optimizing extraction conditions to maximize functionality and allergenicity reduction. The article also highlights the challenges and future perspectives, including optimizing extraction conditions to maximize functionality and allergenicity reduction. The review concludes by highlighting the potential for future research in this area to improve the safety and efficacy of egg-derived peptides more broadly.

show abstract

Section: Fractionation Of Egg Proteinsmentioning

confidence: 99%

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Ovomacroglobulin contains four subunits of equal molecular weight (SDS-PAGE shows about 175 kDa), two of which are disulfide-bonded [ 47 ]. It has been shown that ovomacroglobulin has numerous biological activities, including antibacterial, anti-inflammatory, treatment of keratitis and inhibition of hemagglutinatio [ 48 ].…”

Section: Structures and Functional Properties Of Egg White Proteinsmentioning

confidence: 99%

“…Gel filtration chromatography also provide ideas for separating ovomacroglobulin. The ovomacroglobulin-rich precipitate was first obtained by PEG precipitation, then ovomacroglobulin was purified by two-step chromatography using the Q Sepharose Fast Flow anion-exchange column and the Sephacryl S-200 HR gel filtration column [ 48 ]. From further optimization on this basis, a one-step chromatographic method using a Sephacryl S-200 gel column could achieve 97.0 ± 0.3% purity of ovomacroglobulin [ 63 ].…”

Section: Common Techniquesmentioning

confidence: 99%

Functional Properties and Extraction Techniques of Chicken Egg White Proteins

Huang

Tang

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

Chicken egg whites contain hundreds of proteins, and are widely used in the food, biological and pharmaceutical industries. It is highly significant to study the separation and purification of egg white proteins. This review first describes the structures and functional properties of several major active proteins in egg whites, including ovalbumin, ovotransferrin, ovomucoid, lysozyme, ovomucin, ovomacroglobulin and avidin. Then, the common techniques (including precipitation, chromatography and membrane separation) and some novel approaches (including electrophoresis, membrane chromatography, aqueous two-phase system and molecular imprinting technology) for the separation and purification of egg white proteins broadly reported in the current research are introduced. In addition, several co-purification methods for simultaneous separation of multiple proteins from egg whites have been developed to improve raw material utilization and reduce costs. In this paper, the reported techniques in the last decade for the separation and purification of chicken egg white proteins are reviewed, discussed and prospected, aiming to provide a reference for further research on egg proteins in the future.

show abstract

“…Aqueous two‐phase system (ATPS) is an environmental extraction technology, which has been widely used to the separation and extraction of nucleic acids, proteins, enzymes, drugs, antibiotics, and other biological materials . ATPS has some advantages, such as short processing time, gentle biocompatible environment, and no use of toxic volatile organic solvent in the experiment.…”

Section: Introductionmentioning

confidence: 99%

“…6 Aqueous two-phase system (ATPS) 7,8 is an environmental extraction technology, which has been widely used to the separation and extraction of nucleic acids, 9 proteins, [10][11][12] enzymes, 13 drugs, 14 antibiotics, [15][16][17] and other biological materials. 18,19 ATPS has some advantages, such as short processing time, gentle biocompatible environment, and no use of toxic volatile organic solvent in the experiment. Usual types of ATPS include two different polymers ATPS, 20,21 polymer-salt ATPS, 22,23 ionic liquid (IL)-salt ATPS, 24 and small molecular watermiscible organic solvent-salt ATPS.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Donggang

Wang

et al. 2019

Chirality

View full text Add to dashboard Cite

An environmental and sensitive sample pretreatment method was established and combined with high‐performance liquid chromatography (HPLC) for the analysis of tetracycline hydrochloride (TC) in feed water and lake water. One element small molecule alcohol‐salt aqueous two‐phase system (ATPS) cannot effectively adjust the polarity of the system, but binary small molecule alcohol‐salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. In this work, a binary ATPS based on ethanol +2‐propanol + (NH4)2SO4 system was formed and applied to the separation and purification of TC in real water samples. The influence factors on partition behaviors of TC were discussed, including the types and the concentration of phase salts, the volume ratio of alcohol, the pH value, extraction temperature, and the standing time. The response surface methodology was used to determine the best experimental conditions for multi‐factor experiments. Under this optimal condition, the extraction efficiency of TC reached 95.7%. This new method is considered to have significant application in the divorce of antibiotics.

show abstract

Simple Two-step Chromatographic Method for Purification of Ovomacroglobulin

Cited by 4 publications

References 20 publications

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

Functional Properties and Extraction Techniques of Chicken Egg White Proteins

Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Contact Info

Product

Resources

About