Simple, Rapid, and Selective Isolation of 2S Albumins from Allergenic Seeds and Nuts

Abstract

The 2S albumins belong to the group of seed storage proteins present in different seeds and nuts. Due to their pronounced allergenic potential, which is often associated with severe allergic reactions, this protein family is of special interest in the field of allergen research. Here we present a simple, rapid, and selective method for the purification of 2S albumins directly from allergenic seeds and nuts. We systematically optimized the parameters "buffer system", "extraction temperature", "buffer molarity", and "pH " and were able to achieve 2S albumin purities of about 99% without further purification and demonstrate transferability of this method to nine different allergenic food matrices. Compared to conventional isolation routines, significant reduction of hands-on time and required laboratory equipment is achieved, but nonetheless higher protein yields are obtained. The presented method allows for the rapid purification of different 2S albumins including the corresponding isoforms from natural material.

Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

“…Reports on isolation and characterization of the cashew allergens are scarce. Ana o 3 has been purified before, , but neither purity nor yield were mentioned. Occasionally, references to isolated forms of Ana o 2 (anacardein) are made; , however, as already indicated by the authors themselves, these fractions are not “immunochemically pure” .…”

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

“…2S albumin belongs to the group of prolamins, which have the characteristics of low molecular weight and multiple cysteine residues in their sequence. Furthermore, most allergens in the prolamin group are highly resistant to heat, pH, and gastrointestinal enzymes due to their small and compact structure [137,138]. The 7S legumin is usually a trimeric protein, which has weak stability due to lack of disulfide bonds.…”

The Effect of Consuming Food Allergens on Immune System Function with the Focus on Infectious Lung Diseases (COVID-19)

“…2S albumin is present in many edible grains and is highly considered in allergy-related research due to its allergenic nature [45,46]. In addition to providing plant nutrients, 2S albumin can act as a defensive weapon against fungal attacks.…”