Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Abstract

Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

Multidimensional Applications and Challenges of Riboswitches in Biosensing and Biotherapy

“…[159,160] In recent years, a variety of biosensors have been extensively used to detect food safety. [161,162] Riboswitch-based biosensors can be used for rapid real-time food sample detection with enhanced precision and programmability. [168] Copyright 2014, Elsevier Ltd. B) A fluoride riboswitch-based cell-free sensor using the enzyme colorimetric reporting element.…”

Nanomaterial-Based Fluorescent Biosensor for Food Safety Analysis

“…Numerous aptasensors have been proposed for food safety monitoring, including aptamer-based colorimetric [ 24 ], fluorescent [ 12 , 25 ], electrochemical [ 26 , 27 ], and SPR biosensors [ 28 ]. Wang et al used an aptamer screening kit to rapidly screen aptamers of foodborne Vibrio parahaemolyticus [ 29 ].…”

“…In particular, fluorescent biosensors have attracted considerable attention owing to their simplicity, rapidity, accuracy, and in situ detection [ 11 ]. Furthermore, their spectral properties and fluorescence anisotropy can be used alone or together for the signal output [ 12 ]. These advantages make them suitable for use in food safety analysis.…”

Luminescent Aptamer-Based Bioassays for Sensitive Detection of Food Allergens

“…Fluorescence detection technology has been widely employed for the development of aptasensors for food allergens [ 65 ]. In most cases, the aptamers were labeled with fluorescent dyes or, more recently, nanosized labels, such as quantum dots (QDs), even though assay formats employing unlabeled aptamers are also possible [ 66 , 67 ].…”