Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges

AbuRuz, Salah; Millership, Jeffrey S.; Heaney, Liam G.; McElnay, James

doi:10.1016/j.jchromb.2003.09.044

Cited by 71 publications

(44 citation statements)

References 24 publications

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“…Documented methods for quantitation of the drug utilize gas chromatography [19], radioimmunoassay [20], high performance liquid chromatography (HPLC) [21], spectrophotometry [22] and capillary separations [23] coupled to various detectors, with only few HPLC methods being used for concentration determination of prednisolone in human blood serum and urine. These techniques involve long separation times, use complicated procedures and require derivatization.…”

Section: Introductionmentioning

confidence: 99%

Fullerene C60 modified gold electrode and nanogold modified indium tin oxide electrode for prednisolone determination

Goyal

Oyama

Bachheti

et al. 2009

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

Fullerene C60 modified gold electrode and nanogold modified indium tin oxide electrode for prednisolone determination

Goyal

Oyama

Bachheti

et al. 2009

Bioelectrochemistry

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“…Várias técnicas para a determinação de corticosteróides em fluidos biológicos necessitam do pré-tratamento da amostra [6,9,19,26,34,35,36,37,38,39,40,41] seja através de métodos de extração em fase sólida ou extração por solventes (diclorometano, acetato de etila, metanol, entre outros). Vale ressaltar que o pré-tratamento da amostra pode induzir a alguns problemas, como: o tempo dispensado no processo, manuseio laborioso, erros de manipulação, contaminação por outros agentes, alto custo, decomposição de alguns compostos, entre outros.…”

Section: Corticosterona Acetato De Hidrocortisona Acetato De Dexametaunclassified

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

2010

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The present work had as aim the development of simple an analytical method, fast efficient and the determination of corticosteroids (cortisone, corticosterone, acetate of hidrocortisone and acetate of dexametasone) in sanguine samples of rats using an isocratic system of liquid chromatography of high efficiency (CLAE) with ultraviolet detention. The methodinvolved direct injection of sanguine sample in a chromatographic column with internal surface of phase reverse (ISRP-C18), using the composed mobile phase for drain plug phosphate pH 4,0: acetonitrile (65:35 v/v). The detention the analytes was obtained using an ultraviolet detector of changeable wave length (Varian Model 2550) adjusted in 240 nm and a 4400 integratorSP Chromaject (Varian Associates, Inc., Sunnyvale, CA, USA). The extraction of the analyte resulted in recovery values between 90% and 108%, and variation coefficient enter 1,1% and 2.5%. The detention and quantification limits of the method were 0,02 and 0,04 μg mL-1, respectively. In conclusion, the analytical method possible the direct injection (on-line) of the sample without previous treatment also presenting some advantages, such as: fast, exactness, precision and specific.

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“…Urinary cortisol was extracted (solid-phase extraction) and analysed using highperformance liquid chromatography (HPLC) [17]. This method has been shown to have high specificity for cortisol measurement without interference from prednisolone or inhaled steroids and has been shown to be superior to radioimmunoassay and enzyme-linked immunosorbent assay [18] due to a higher degree of specificity.…”

Section: Chromatographic Analysismentioning

confidence: 99%

A cross‐sectional study evaluating the relationship between cortisol suppression and asthma control in patients with difficult asthma

AbuRuz

Heaney

Millership

et al. 2006

Brit J Clinical Pharma

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AimTo examine the relationship between cortisol suppression and asthma symptoms in patients with difficult asthma. MethodsPatients, referred to a specialist difficult asthma service and who fulfilled the criteria for difficult asthma, were recruited to the study in a sequential, unselected manner. At each clinic visit, all patients completed a validated asthma control questionnaire. For measuring cortisol suppression, early morning urinary cortisol [corrected for creatinine to give urinary cortisol creatinine ratio (UCC ratio)] was used. The urine samples were collected and stored at − 70 ° C until ready for analysis. Urinary cortisol was extracted (solid-phase extraction) and analysed using high-per formance liquid chromatography. The Pearson correlation coefficient was used for correlation analysis while t -tests were used for between-group differences for normally distributed data. If the data were not normally distributed, nonparametric statistics were used. A Pvalue < 0.05 was considered statistically significant. ResultsDuring the study period all the patients who attended the difficult asthma clinic and fulfilled the criteria for difficult asthma ( n = 66) agreed to take part in the study. There were moderate to strong and significant associations between several measures of asthma control and UCC ratio. The correlation coefficient with five indicators of asthma control ranged between 0.3 and 0.5 ( P < 0.05). ConclusionsWe have demonstrated a relationship between cortisol suppression and asthma control in difficult asthmatics on high-dose steroid therapy. We have proposed a model based on the relationship between symptom control and cor tisol suppression, whereby both adherence and therapeutic adjustments could potentially be made. A properly controlled prospective clinical trial should examine the utility of this approach in clinical practice.

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Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges

Cited by 71 publications

References 24 publications

Fullerene C60 modified gold electrode and nanogold modified indium tin oxide electrode for prednisolone determination

Fullerene C60 modified gold electrode and nanogold modified indium tin oxide electrode for prednisolone determination

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

A cross‐sectional study evaluating the relationship between cortisol suppression and asthma control in patients with difficult asthma

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