Simple determination of terbutaline in dog plasma by column-switching liquid chromatography

Zhang, Y.; Zhang, Z.R.

doi:10.1016/j.jchromb.2004.02.037

Cited by 15 publications

(9 citation statements)

References 14 publications

(23 reference statements)

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“…C14H20N2Br2·HCl. A number of methods have been developed for the estimation of bromhexine individually and also in combined forms along with other drugs which include HPLC [10][11][12][13][14][15], spectrophotometry [16][17][18][19][20], fluorimetry [21]. Oxtriphylline is a bronchodilator used to treat the symptoms of asthma, bronchitis and emphysema.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Salbutamol, Oxtriphylline and Bromhexine Hydrochloride in Tablet Dosage Form by RP-HPLC

Chakravarthi¹,

Devanna²

2017

Asian J. Chem.

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A simple, rapid, accurate, specific and stability indicating reverse phase HPLC method has been developed and validated for the simultaneous estimation of salbutamol, oxtriphylline and bromhexine hydrochloride in bulk drug and pharmaceutical tablet dosage form. The chromatographic separation was performed on the kromasil C18 column (250 mm × 4.6 mm, 5 µm particle size), using a mobile phase of orthophosphoric acid buffer:acetonitrile (65:35 v/v), at a flow rate of 1.0 mL/min at an ambient temperature of 25 °C with the detection wavelength at 260 nm. The retention times of salbutamol, oxtriphylline and bromhexine hydrochloride was found to be 2.03, 2.90 and 4.92 min, respectively. The proposed method has been validated for linearity, range, precision, accuracy and robustness were within the acceptance limit according to ICH Q2R1 guidelines. Quantification of the components in actual tablet formulations was calculated against the responses of freshly prepared external standard solutions. In the linearity test correlation coefficient was found to be 0.999 for all the molecules, percentage relative standard deviation (RSD) results from precision studies were 0.7, 0.2 and 0.3; mean percentage recoveries in accuracy studies were found to be 99.33, 99.44 and 99.36 % for salbutamol, oxtriphylline and bromhexine hydrochloride, respectively. Very low concentrations of LOD and LOQ indicate the method was highly sensitive enough. The designed validated method can be used effectively in the laboratory for regular determination of salbutamol, oxtriphylline and bromhexine hydrochloride in tablet formulation and bulk form.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Salbutamol, Oxtriphylline and Bromhexine Hydrochloride in Tablet Dosage Form by RP-HPLC

Chakravarthi¹,

Devanna²

2017

Asian J. Chem.

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“…Within the EU, their use for growth promotion has been banned since 1988 [3]. Cimaterol, salbutamol, terbutaline, and ractopamine belong to the class of b-agonist drugs [4,5], and are the derivatives of phenyl-b-ethanol amines with different substituents on the aromatic ring and on the terminal amino group [6]. Their basic information is listed in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Cimaterol, Salbutamol, Terbutaline and Ractopamine in Feed by SPE Coupled to UPLC

Zhang

et al. 2011

Chromatographia

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An ultra performance liquid chromatography (UPLC) method was established for the simultaneous determination of cimaterol, salbutamol, terbutaline, and ractopamine in feed. Target compounds were extracted with methanol and centrifuged. The supernatant was then transferred and concentrated, and applied to a solid phase extraction MCX cartridge for clean-up before UPLC analysis. The calibration curves for the four b-agonists were good linear in concentration range of 0.05-1.0 lg mL -1 with the correlation coefficients (r) over 0.999. The average recoveries were in the range of 90.1-101.4% with relative standard deviation values lower than 8.0%.

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“…Peak plasma levels are 1.2 lg mL À1 for every mg of an oral dose, reached within 2-3 h. Following inhalation, only about 10-20% of inhaled dose reaches the lungs but after nanosizing the drug candidate >50% can be targeted deeper to alveolar region (Bhavna et al, 2009). Several analytical techniques like, ultraviolet spectroscopy (Selek et al, 2003;Shahin et al, 2002), voltammetery (Beltagi et al, 2007), capillary electrophoresis (Boer and Ensing, 1998;Kim et al, 2000), chemiluminescence (Li et al, 2009), high performance liquid chromatography (Chiap et al, 2002;Herring and Johnson, 2000;Jacobson and Peterson, 1994;Reverchon and Porta, 2003;Sagar et al, 1993), LC-electrochemical detection (Edholm et al, 1984;Zhang and Zhang, 2004), LC-tandem MS Fesser et al, 2005), GC-MS (Spisso et al, 2000), and electrospray high-field asymmetric waveform ion mobility spectrometry-mass spectrometry (ESI-FAIMS-MS) (Mie et al, 2008) have been reported in the literature for quantification of TBS; but most of them either employ large sample volumes or have long extraction processes. For pharmacokinetic studies, the most useful bioanalytical method reported is GC-MS (Borgstro¨m et al, 1989;Spisso et al, 2000;Bredberg et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

A validated HPTLC method for determination of terbutaline sulfate in biological samples: Application to pharmacokinetic study

Faiyazuddin

Rauf

Ahmad

et al. 2011

Saudi Pharmaceutical Journal

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Terbutaline sulfate (TBS) was assayed in biological samples by validated HPTLC method. Densitometric analysis of TBS was carried out at 366 nm on precoated TLC aluminum plates with silica gel 60F254 as a stationary phase and chloroform-methanol (9.0:1.0, v/v) as a mobile phase. TBS was well resolved at RF 0.34 ± 0.02. In all matrices, the calibration curve appeared linear (r (2) ⩾ 0.9943) in the tested range of 100-1000 ng spot(-1) with a limit of quantification of 18.35 ng spot(-1). Drug recovery from biological fluids averaged ⩾95.92%. In both matrices, rapid degradation of drug favored and the T 0.5 of drug ranged from 9.92 to 12.41 h at 4 °C and from 6.31 to 9.13 h at 20 °C. Frozen at -20 °C, this drug was stable for at least 2 months (without losses >10%). The maximum plasma concentration (Cpmax) was found to be 5875.03 ± 114 ng mL(-1), which is significantly higher than the maximum saliva concentration (Csmax, 1501.69 ± 96 ng mL(-1)). Therefore, the validated method could be used to carry out pharmacokinetic studies of the TBS from novel drug delivery systems.

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Simple determination of terbutaline in dog plasma by column-switching liquid chromatography

Cited by 15 publications

References 14 publications

Simultaneous Estimation of Salbutamol, Oxtriphylline and Bromhexine Hydrochloride in Tablet Dosage Form by RP-HPLC

Simultaneous Estimation of Salbutamol, Oxtriphylline and Bromhexine Hydrochloride in Tablet Dosage Form by RP-HPLC

Simultaneous Determination of Cimaterol, Salbutamol, Terbutaline and Ractopamine in Feed by SPE Coupled to UPLC

A validated HPTLC method for determination of terbutaline sulfate in biological samples: Application to pharmacokinetic study

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