Simultaneous Determination of Cimaterol, Salbutamol, Terbutaline and Ractopamine in Feed by SPE Coupled to UPLC

Abstract

An ultra performance liquid chromatography (UPLC) method was established for the simultaneous determination of cimaterol, salbutamol, terbutaline, and ractopamine in feed. Target compounds were extracted with methanol and centrifuged. The supernatant was then transferred and concentrated, and applied to a solid phase extraction MCX cartridge for clean-up before UPLC analysis. The calibration curves for the four b-agonists were good linear in concentration range of 0.05-1.0 lg mL -1 with the correlation coefficients (r) over 0.999. The average recoveries were in the range of 90.1-101.4% with relative standard deviation values lower than 8.0%.

Determination of beta‐agonists in swine hair by μFIA and chemiluminescence

“…The tolerable concentration ratios with respect to 1.0 × 10 −6 mol/L ␤-agonists for interference at the 5% level were over 1000 for Na + , K + , Cl − , NO 3− , Glucose, Starch, and EDTA; 100 for Alanine, Phenylalanine, Glycine, and Tryptophan; 50 for SO 4 2− , Mg 2+ and CO 3 2+ ; 10 for Cystine, Methionine, and Urea; 5 for Ca 2+ and Zn 2+ ; and 1 for Cu 2+ , Fe 3+ , Fe 2+ , and Mn 2+ . The main interferences are from heavy metal ions, which can be eliminated by using EDTA as a masking reagent.…”

“…So ␤-agonists have been used as growth promoters in the livestock industry. However, once the animals were fed with the ␤-agonists, the residues may remain in their meat and liver for a long time and it is difficult to eliminate them [4] by heating in the generally cooking process, and therefore this group of feed addictives eventually lead to a potential risk to human health. For the sake of consumer's health, many countries, including the European Union, China, and Taiwan, have a zero-tolerance policy for meats with even traces of these substances.…”

Screening, recognition and quantitation of salbutamol residues in ham sausages by molecularly imprinted solid phase extraction coupled with high-performance liquid chromatography–ultraviolet detection

“…In order to analysis of SAL in biological samples, several methods have been developed, such as high performance liquid chromatography (HPLC) [1,7,8], capillary electrophoresis (CE) [9][10][11], gas chromatography-mass spectrometry (GC-MS) [12], liquid chromatography-mass spectrometry (LC-MS) [13,14], enzyme immunoassay [15], and bromatometric methods [16]. However, they are suffered to achieve separation of target compounds from complex sample matrices directly without the pretreatment process.…”

“…Recently, owing to the large numbers of food safety events, among which was "lean meat powder" toxic incident, occurred one after another, the problem of food safety has aroused in the world scope and become the focus of the government and the customers [1]. Salbutamol (SAL) is a selective ␤ 2 -adrenoceptor agonist extensively used as a bronchodilator in asthmatic patients [2].…”

Determination of ractopamine in pork by using electrochemiluminescence inhibition method combined with molecularly imprinted stir bar sorptive extraction

“…Many methods for RAC determination have been reported [7][8][9][10][11][12][13][14], including liquid chromatography (LC), gas chromatography (GC) and both methods coupled with mass spectrometry (MS) detection. These methods usually require expensive instrumentation and cumbersome sample pretreatments; sometimes have less sensitive and narrow linear response range, and suffer from some interference.…”