Simple Assay for Accurate Determination of [
            <sup>35</sup>
            S]sulfate Reduction Activity

Rosser, H.R.; Hamilton, W. Allan

doi:10.1128/aem.45.6.1956-1959.1983

Cited by 38 publications

(13 citation statements)

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“…T h e activity of SRB within alginate beads was measured in terms of hydrogen sulphide generation, determined using an adapted radiorespirometric assay (Rosser and Hamilton 1983;Hardy and Syrett 1983). T h e following details are pertinent to the use of alginate beads.…”

Section: Activity Of Srb Within Stored Artificial Biofilm Beadsmentioning

confidence: 99%

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Whitham¹,

Gilbert²

1993

Journal of Applied Bacteriology

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R T. 1993. A model artificial biofilm was developed and evaluated for ranking the performance of biocides for application in oil production pipelines. T h e biofilm consisted of an alginate gel matrix into which were incorporated bacteria, scrapings from the inner surfaces of oil production pipelines and some crude oil.T h e viability and sulphide-respiration rates of sulphate-reducing bacteria (SRB) within freshly-prepared artificial biofilm remained largely unchanged during a 2-week storage period. Furthermore, storage of the model biofilm did not alter the susceptibility of the incorporated SRB to a biocide. These findings showed that artificial biofilm may be produced in advance of a biocide assessment study and stored for at least 2 weeks over the course of the study without t h e model system undergoing changes which affected t h e relative performance of the biocides assessed. Artificial biofilms were found to be more resistant to biocides than planktonic bacteria and the addition of oil pipeline scrapings and crude oil to the artificial biofilm was found to increase further the resistance of biofilm to biocides.

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Section: Activity Of Srb Within Stored Artificial Biofilm Beadsmentioning

confidence: 99%

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Whitham¹,

Gilbert²

1993

Journal of Applied Bacteriology

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“…In a comparison among radiotracer techniques, Meier et al (2000) discussed three different procedures to quantify sulfate reduction ratestwo passive extractions, as described by Rosser and Hamilton (1983) and Ulrich et al (1997), and reflux distillation, as suggested by Fossing and Jorgensen (1989). They concluded that sulfate reduction rates are reproducible both for passive and active extraction methods for recovery of reduced 35 S-sulfur.…”

Section: Discussionmentioning

confidence: 99%

“…They consider that the diffusion procedure of Ulrich et al (1997) is a quick and simple method for the extraction of total reduced inorganic sulfurs, with a good efficiency, comparable with that of the distillation procedure. Maxwell and Hamilton (1986) used the method proposed by Rosser and Hamilton (1983) with the addition of a 3Á0 9 1Á0 9 0Á5 cm 50D mild steel corrosive coupon. They described that the applicability of this assay to high sulfide production systems, as water injection systems and oil storage cells, must be carefully assessed.…”

Section: Discussionmentioning

confidence: 99%

“…Authors observed that with a contact time of 2 h, it was possible to observe distinct inhibiting effects of quaternary ammonium compound-based products. The technique could be used to assess inhibitor efficiencies in <6 h. Rosser and Hamilton (1983) detected labelled reduced sulfur species in sediments contaminated with domestic and industrial effluents, with an efficiency of 99Á3%. Maxwell (1986) used the radiorespirometric technique to assess SRB-mediated corrosion concluding that it is not possible to correlate microbial production of sulfide and corrosion rates, due to the chemical and physical nature of the materials tested, that play a more important role.…”

Section: Discussionmentioning

confidence: 99%

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Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria

2013

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Aims: The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H 2 S in trace concentrations, to correlate with sulfate-reducing bacterial activity. Methods and Results: Serial dilutions of synthetic sea water with a pure culture of Desulfovibrio alaskensis, a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction. Conclusions: Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum. Significance and Impact of the Study: The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.

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“…An alternative method to separate sulfide from sulfate is to place a sulfide trap within a sealed vial where acidification and reduction by Cr 2+ is performed. This method relies on passive diffusion to transport H 2 S from the slurry of sediment and reagents, and into the trap (Rosser and Hamilton 1983;Hsieh and Yang 1989). However, the passive distillation suffers from low recovery (Howarth and Giblin 1983), especially for elemental sulfur (Ulrich et al 1997).…”

Section: -mentioning

confidence: 99%

Determination of dissimilatory sulfate reduction rates in marine sediment via radioactive ³⁵S tracer

Røy

Weber

Tarpgaard

et al. 2014

Limnology & Ocean Methods

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The concentration of sulfate in seawater exceeds all other dissolved electron acceptors combined by more than an order of magnitude. This allows dissimilatory sulfate reduction to persist in marine sediment long after the reactive species of more energetically favorable electron acceptors have been depleted. Thus, sulfate reduction dominates anaerobic carbon oxidation in most coastal and estuarine sediments worldwide (Goldhaber and Kaplan 1975;Jørgensen 1977 Jørgensen , 1982Canfield 1993). The relative importance of sulfate reduction decreases at low sedimentation rates because slowly deposited sediments are exposed to oxygen, nitrate and oxidized metals for a longer time after deposition, and because the degradability of dead organic matter decreases steeply during aging (Middelburg 1989). Sulfate reduction in deep-sea sediments therefore only contribute minutely to the global sulfur cycling (Canfield et al. 2005; Jørgensen and Kasten 2006) but deeply buried sediments with low metabolic activity still comprise a vast volume and the carbon mineralization in these sediments exerts major control on the climate and the chemistry of the planet.Direct determination of sulfate reduction rates by measuring the decreasing sulfate concentration over time in a closed vial is possible in limnic sediment (e.g., Bak et al. 1991), but it is not practical in marine sediments due to the high sulfate concentration in seawater (28 mmol L -1 ). Typical sulfate reduction rates in coastal surface-sediments are in the order of 20 nmol SO 4 2-cm -3 d -1 (Jørgensen 1982). With near 20 μmol SO 4 2-cm -3 in the sediment this implies that a 1% reduction in sulfate content requires impractically long incubation for days to months. The slow relative change in sulfate concentration is, however, turned to an advantage with radiotracer-based methods because the specific activity of the tracer remains nearly constant throughout the experiment. Because only the added tracer is measured, the high background concentration of sulfate and sulfides is of little concern. Here we re-evaluate the 35 SO 4 2-based measurement of sulfate reduction in marine sediments that has developed into a standard method over the last 30 years. Theoretical basisThe high activation energy of SO 4 2-makes microbial reduction through enzymatic catalysis the only pathway of sulfate reduction within the physiological temperature range in marine sediments. Various organisms are capable of assimila- AbstractRates of dissimilatory sulfate reduction in aquatic sediments have been measured over many years with 35 Sradiotracer, and the method has been continuously modified and optimized. This article discusses the sequence of procedures that constitutes the method from sediment handling before incubation, via incubation and distillation, to statistical analysis of the results. We test modifications that have been added since previous method descriptions, and we recommend sound experimental procedures. We discuss the measurement of extremely low sulfate reduction rates whereby o...

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Simple Assay for Accurate Determination of [ ³⁵ S]sulfate Reduction Activity

Cited by 38 publications

References 15 publications

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria

Determination of dissimilatory sulfate reduction rates in marine sediment via radioactive ³⁵S tracer

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Simple Assay for Accurate Determination of [ 35 S]sulfate Reduction Activity

Cited by 38 publications

References 15 publications

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Evaluation of a model biofilm for the ranking of biocide performance against sulphate‐reducing bacteria

Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria

Determination of dissimilatory sulfate reduction rates in marine sediment via radioactive 35S tracer

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Product

Resources

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Simple Assay for Accurate Determination of [ ³⁵ S]sulfate Reduction Activity

Determination of dissimilatory sulfate reduction rates in marine sediment via radioactive ³⁵S tracer