Silver/polysaccharide-based nanofibrous materials synthesized from green chemistry approach

Martínez-Rodríguez, M.A.; Garza-Navarro, Marco A.; Moreno-Cortez, Iván E.; Porto, Raúl Lucio; González-González, V.

doi:10.1016/j.carbpol.2015.09.014

Cited by 26 publications

(14 citation statements)

References 39 publications

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“…This widening has been attributed to the superposition of the stretching vibration of OH functional groups in the PVA polymer chain and the N–H amide groups in the HRP protein structure . The previous experimental evidence confirms the presence of the HRP enzyme in the PVA nanofibers.…”

Section: Resultsmentioning

confidence: 54%

Thermal stability of the immobilization process of horseradish peroxidase in electrospun polymeric nanofibers

Rodríguez-deLuna

Moreno-Cortez

Garza-Navarro

et al. 2017

J of Applied Polymer Sci

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The horseradish peroxidase enzyme (type VI 250 U mg−1) was encapsulated in polymeric nanofibers using the electrospinning technique and successfully immobilized by the exposure to glutaraldehyde (GA) vapor in order to create covalent bonds between the polyivinilalcohol (PVA) polymeric chains and the enzyme molecules. The morphology of the nanofibers was analyzed by scanning electron microscopy (SEM) showing a diameter in the range of 100–200 nm. The presence of the enzyme in the electrospun nanofibers was confirmed by infrared spectroscopy (FTIR). The optimum crosslinking time was 1 h of exposure to GA vapor. The maximum percentage of the retained protein and the enzyme activity was obtained using the lowest initial enzyme concentration. The enzyme activity of the sample was retained after four reuse cycles. Differential scanning calorimetry (DSC) was used to study the thermal stability of the samples. The thermal study of the immobilized HRP enzyme provide for the first time additional information regarding the interaction of the HRP enzyme molecules with the PVA chains of the nanofiber matrix, as well as the effect of the crosslinking time in the glass transition temperature and the heat of fusion of the samples. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44811.

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Section: Resultsmentioning

confidence: 54%

Thermal stability of the immobilization process of horseradish peroxidase in electrospun polymeric nanofibers

Rodríguez-deLuna

Moreno-Cortez

Garza-Navarro

et al. 2017

J of Applied Polymer Sci

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“…The CMC is usually commercialized as a water-soluble sodium salt, which in aqueous solution can be loaded with metallic ions as Ag + by a simple displacement reaction of Na + [18]. Moreover, due to the abundant hydroxyl groups on its molecular structure, CMC has been successfully used as a reducing agent for the preparation of CMC-AgNPs composites [16,19]. From this approach is possible to get an outstanding particle size control and good efficiency over the silver ions reduction, without the use or generation of hazardous substances.…”

Section: Introductionmentioning

confidence: 99%

Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

et al. 2020

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In this paper we report on the influence of polysaccharides’ molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC). These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw). The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques. The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis. The cytotoxicity of composites was assessed against human gingival fibroblast. Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation. This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO− moieties of CMC. Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.

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“…However, it was determined from the P ‐value results, the coefficients of the model equation and the one parameter at a time graphs that the fiber diameter size decreased linearly with the increase of feed rate as given in Figure D‐F. This may be related to the presence of silver nanoparticles and bioactive glass, which increases the conductivity of the solution . In this case, it is possible that the feed rate cannot overcome the fiber diameter reduction effect of silver nanoparticles and bioactive glass …”

Section: Resultsmentioning

confidence: 99%

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

2020

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The current research focuses on the findings of an investigation on optimizing of an electrospun antibacterial gelatin nanocomposite membrane for bone tissue engineering applications. For this reason, soluble starch coated silver nanoparticles (Ag‐NPs) and bioactive glass particles (BG) were incorporated in to gelatin (Gt) to fabricate Gt/Ag‐NPs/BG nanocomposite membranes. Employing Box‐Behnken design, second‐order models have been successfully obtained to evaluate the statistical significance of individual and interaction effects of applied voltage, tip‐to‐collector distance (TCD), and flow rate on fiber diameter. There was a reasonable agreement between the regression R2 value (0.9542), R2 predicted value (0.8768), and the R2 adjusted value (0.9286) across the entire factor space with identical observations for the experimental and model values. Under optimum conditions (applied voltage of 26 kV, TCD of 180 mm, and flow rate of 0.5 mL/h), the nanocomposite membrane with similar fiber size of bone tissue extracellular matrix can be fabricated with the predicted value of 557 nm obtained by the proposed model. The optimized nanofiber membrane was fabricated under these conditions and average fiber diameter of this membrane was found as 472 ± 94 nm. The characterization studies of this nanofiber suggest that obtained nanocomposite is a potential candidate for bone tissue engineering applications.

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Silver/polysaccharide-based nanofibrous materials synthesized from green chemistry approach

Cited by 26 publications

References 39 publications

Thermal stability of the immobilization process of horseradish peroxidase in electrospun polymeric nanofibers

Thermal stability of the immobilization process of horseradish peroxidase in electrospun polymeric nanofibers

Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

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