Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

Martínez-Rodríguez, M.A.; Madla-Cruz, Elizabeth; Urrutia-Baca, Víctor Hugo; Garza-Ramos, M. de la; González-González, V.; Garza-Navarro, Marco A.

doi:10.3390/nano10061164

Cited by 32 publications

(8 citation statements)

References 45 publications

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“…[34][35][36][37] Previously few studies reported the synthesis of CMC fabricated silver NPs through chemical reduction methods. 38,39 However, there is no study available on the decoration of silver NPs with CMC via green route using Syzygium aromaticum buds extracts. In addition, no study reported the pharmacological and cytotoxic activities of CMC-based silver NPs.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Asghar

Yousuf

Shoaib

et al. 2021

IJN

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Purpose The current study proposed the simple, eco-friendly and cost-effective synthesis of carboxymethyl cellulose (CMC) structured silver-based nanocomposite (CMC-AgNPs) using Syzygium aromaticum buds extract. Methods The CMC-AgNPs were characterized by ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transmission infra-red (FTIR), energy-dispersive X-ray (EDX), and dynamic light scattering (DLS) techniques. The synthesized nanocomposites were evaluated for their bactericidal kinetics, in-vivo anti-inflammatory, anti-leishmaniasis, antioxidant and cytotoxic activities using different in-vitro and in-vivo models. Results The spherical shape nanocomposite of CMC-AgNPs was synthesized with the mean size range of 20–30 nm, and the average pore diameter is 18.2 nm while the mean zeta potential of −31.6 ± 3.64 mV. The highly significant (P < 0.005) antibacterial activity was found against six bacterial strains with the ZIs of 24.6 to 27.9 mm. More drop counts were observed in Gram-negative strains after 10 min exposure with CMC-AgNPs. Significant damage in bacterial cell membrane was also observed in atomic force microscopy (AFM) after treated with CMC-AgNPs. Nanocomposite showed highly significant anti-inflammatory activity in cotton pellet induced granuloma model (Phase I) in rats with the mean inhibitions of 43.13% and 48.68% at the doses of 0.025 and 0.05 mg/kg, respectively, when compared to control. Reduction in rat paw edema (Phase II) was also highly significant (0.025 mg/kg; 42.39%; 0.05 mg/kg, 47.82%). At dose of 0.05 mg/kg, CMC-AgNPs caused highly significant decrease in leukocyte counts (922 ± 83), levels of CRP (8.4 ± 0.73 mg/mL), IL-1 (177.4 ± 21.3 pg/mL), IL-2 (83.7 ± 11.5 pg/mL), IL-6 (83.7 ± 11.5 pg/mL) and TNF-α (18.3 ± 5.3 pg/mL) as compared to control group. CMC-AgNPs produced highly effective anti-leishmaniasis activity with the viable Leishmania major counts decreased up to 36.7% within 24 h, and the IC 50 was found to be 28.41 μg/mL. The potent DPPH radical scavenging potential was also observed for CMC-AgNPs with the IC 50 value of 112 μg/mL. Furthermore, the cytotoxicity was assessed using HeLa cell lines with the LC 50 of 108.2 μg/mL. Conclusion The current findings demonstrate positive attributes of CMC fabricated AgNPs as a promising antibacterial, anti-inflammatory, anti-leishmaniasis, and antioxidant agent with low cytotoxic potential.

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Section: Introductionmentioning

confidence: 99%

Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Asghar

Yousuf

Shoaib

et al. 2021

IJN

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“…Composites of CMC of different degree of substitution (DS) and molecular weight (Mw) containing stabilized AgNPs were green synthesized from the reduction of Ag + into aqueous solutions of the polysaccharide by using chemical method. Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver nitrate added to the reaction, as well as the DS and Mw of Na‐CMC used for composites preparation 12 …”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties and antimicrobial activity of nanocomposite films based on carboxymethylcellulose and silver nanoparticles

Yunusov

Sarymsakov

Jalilov

et al. 2021

Polymers for Advanced Techs

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Silver nanoparticles (AgNPs) stabilized by sodium carboxymethylcellulose (Na‐CMC) with degree of substitution (DS) 0.65‐0.90 and degree of polymerization (DP) 200‐600 were synthesized by photochemical method. The structural, physico‐chemical, and physico‐mechanical properties as well as antimicrobial activity of polymer films containing the Na‐CMC and AgNPs were studied. The shape, quantity, and size of the AgNPs embedded into the Na‐CMC films were determined by UV‐Vis spectroscopy, ZETASIZER Nano ZS, atomic force microscopy and transmission electron microscopy. It was found that the increase of silver nitrate concentration in solution of Na‐CMC followed by photoirradiation leads to the change of size and shape of AgNPs. The AgNPs in the range of size from 5 to 35 nm were found to enhance the microbicidal activities of the Na‐CMC films.

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“…Even if they possess remarkable therapeutic effects and intrinsic biocompatibility, relentless efforts were done to maximize the bioactivity of AgNPs and improve their stability. In this respect, a wide variety of capping agents and surface coatings were successfully evaluated, including inorganic or organic, natural or synthetic materials [ 25 , 26 , 27 , 28 ].…”

Section: ⧉ Introductionmentioning

confidence: 99%

Biodistribution of essential oil-conjugated silver nanoparticles

Gherasim

Grumezescu²,

Mogoşanu³

et al. 2021

Rom J Morphol Embryol

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The beneficial synergy between antimicrobial silver nanoparticles (AgNPs) and essential oils (EOs), with therapeutic effects that have been acknowledged and explored for a long time, opens the way towards developing new and promising alternatives for anti-infective therapies. With the aim to improve the cytocompatibility and stability of AgNPs and to overcome the volatilization of EOs, AgNPs conjugated with sage (Salvia officinalis) and cinnamon (Cinnamomum aromaticum) EOs were obtained in our study. The synthesis process was realized either by classical or ultrasound-assisted chemical reduction. Compositional and microstructural characterization of the as-obtained Ag@EO NPs was performed by X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The biodistribution of Ag@EO NPs was evaluated on a mouse animal model.

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Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

Cited by 32 publications

References 45 publications

Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Physicochemical properties and antimicrobial activity of nanocomposite films based on carboxymethylcellulose and silver nanoparticles

Biodistribution of essential oil-conjugated silver nanoparticles

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