Silver nanoparticles enhance wound healing in zebrafish (Danio rerio)

Abstract

Silver nanoparticles (AgNPs) were successfully synthesized by a chemical reduction method, physico-chemically characterized and their effect on wound-healing activity in zebrafish was investigated. The prepared AgNPs were circular-shaped, water soluble with average diameter and zeta potential of 72.66 nm and -0.45 mv, respectively. Following the creation of a laser skin wound on zebrafish, the effect of AgNPs on wound-healing activity was tested by two methods, direct skin application (2 μg/wound) and immersion in a solution of AgNPs and water (50 μg/L). The zebrafish were followed for 20 days post-wounding (dpw) by visual observation of wound size, calculating wound healing percentage (WHP), and histological examination. Visually, both direct skin application and immersion AgNPs treatments displayed clear and faster wound closure at 5, 10 and 20 dpw compared to the controls, which was confirmed by 5 dpw histology data. At 5 dpw, WHP was highest in the AgNPs immersion group (36.6%) > AgNPs direct application group (23.7%) > controls (18.2%), showing that WHP was most effective in fish immersed in AgNPs solution. In general, exposure to AgNPs induced gene expression of selected wound-healing-related genes, namely, transforming growth factor (TGF-β), matrix metalloproteinase (MMP) -9 and -13, pro-inflammatory cytokines (IL-1β and TNF-α) and antioxidant enzymes (superoxide dismutase and catalase), which observed differentiation at 12 and 24 h against the control; but the results were not consistently significant, and many either reached basal levels or were down regulated at 5 dpw in the wounded muscle. These results suggest that AgNPs are effective in acceleration of wound healing and altered the expression of some wound-healing-related genes. However, the detailed mechanism of enhanced wound healing remains to be investigated in fish.

Mechanical induced wounds in fish – a review on models and healing mechanisms

“…2013; Seo et al . 2017). However, the cost and availability of the equipment may limit the implementation of this method.…”

Oral Administration of Alginate Oligosaccharide from Padina sp. Enhances Tolerance of Oxygen Exposure Stress in Zebrafish (Danio rerio)

“…The feeding trial was reared for twelve days. During this rearing period, Zebrafish (D. rerio) were fed up 4% of biomass at three times.day -1 (Seo et al, 2017).…”

<p>Silver Decorated Mesoporous Carbons for the Treatment of Acute and Chronic Wounds, in a Tissue Regeneration Context</p>

“…In fact, several works report Ag nanoparticles inducing higher collagen expression, 34 promoting re-epithelialization through increased cell migration and proliferation 35 and accelerating wound closure. 35,36 Given that macrophages were shown to be activated by C3 nanomatrices (with and without Ag) with proinflammatory cytokines' production, 500 μL MɸCM were added to a fibroblast cell culture and RT-qPCR was performed after 48 hrs incubation time. Expression of the main tissue repair genes, such as FGF, EGF, TGF-β1, Col1a1, Spp1, VEGFa, MMP-9 and TIMP-3 were investigated, in order to evaluate the wound healing potential of the pristine and silver-doped mesoporous carbons ( Figure 10).…”