Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation

Abstract

The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various strategies have been tried. It remains a challenge to obtain good cell proliferation and antibacterial effects. Here, human hair kerateine (HHK)/poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) nanofibers were prepared using cysteine-rich HHK, and then, silver nanoparticles (AgNPs) were in situ anchored in the sulfur-containing amino acid residues of HHK. After the ultrasonic degradation test, HHK/PEO/PVA nanofibrous mats treated with 0.005-M silver nitrate were selected due to their relatively complete structures. It was observed by TEM-EDS that the sulfur-containing amino acids in HHK were the main anchor points of AgNPs. The results of FTIR, XRD and the thermal analysis suggested that the hydrogen bonds between PEO and PVA were broken by HHK and, further, by AgNPs. AgNPs could act as a catalyst to promote the thermal degradation reaction of PVA, PEO and HHK, which was beneficial for silver recycling and medical waste treatment. The antibacterial properties of AgNP-HHK/PEO/PVA nanofibers were examined by the disk diffusion method, and it was observed that they had potential antibacterial capability against Gram-positive bacteria, Gram-negative bacteria and fungi. In addition, HHK in the nanofibrous mats significantly improved the cell proliferation of NIH3T3 cells. These results illustrated that the AgNP-HHK/PEO/PVA nanofibrous mats exhibited excellent antibacterial activity and the ability to promote the proliferation of fibroblasts, reaching our target applications.

Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation

“…By increasing the CEO-loaded keratin to 0.5 mg/mL, the cell viability decreased [ 89 ]. The materials applied in the study PU, Gel, and PVA as electrospun NFs were reported for their support for cell adhesion, growth, and proliferation, where they simulate the structure of a natural extracellular matrix (ECM) [ 39 , 90 ].…”

Synthesis and modification of the structural, optical, and thermal properties of PVA–PEO by LDH nanoplates

“…It can be inferred from the position and wideness of this peak that increasing LDH content up to 7.0 wt% promotes the hydrophilicity of the PVA-PEO blend to enhance this endothermic peak [47]. Tang et al [19] also reported that the water evaporation from PVA-PEO mixed with human hair kerateine took place at ∼167 °C-196 °C. But this peak disappeared after the addition of AgNO 3 , where the Ag replaced the water molecules.…”

“…Additionally, Gouda et al [17] achieved some improvements in the thermal, mechanical properties, and selectivity of the PVA (85%)/PEO (15%) blend, compared with those of the Nafion117 membrane, by doping with 3.0 wt% PO 4 TiO 2 nanotubes. Moreover, the thermal, hydrophobic, and mechanical properties and cytotoxicity of keratin/PVA/ PEO nanocomposites were investigated under certain conditions [18,19].…”

Perspectives on Usage of Functional Nanomaterials in Antimicrobial Therapy for Antibiotic-Resistant Bacterial Infections

“…Necrotic tissue helps in the adhesion of bacteria in these infections while also providing nutrients that delay the healing of wounds by preventing re-epithelialization and extending inflammation. The treatment of wound infections utilizes AgNPs that have been integrated into hydrogels or wound dressings . Additional types of nanoparticles have also been the subject of increasing research for treating wounds affected by biofilm.…”

“…The treatment of wound infections utilizes AgNPs that have been integrated into hydrogels or wound dressings. 154 Additional types of nanoparticles have also been the subject of increasing research for treating wounds affected by biofilm. For instance, P. aeruginosa and S. aureus biofilms could not form when copper particles were inserted into biodegradable nanofibers, and any biofilms that had already grown were destroyed by the copper particles.…”