Silver nanoclusters based FRET aptasensor for sensitive and selective fluorescent detection of T-2 toxin

Khan, Imran Mahmood; Zhao, Sen; Niazi, Sobia; Mohsin, Ali; Shoaib, Muhammad; Duan, Nuo; Wu, Shijia; Wang, Zhouping

doi:10.1016/j.snb.2018.09.021

Cited by 77 publications

(27 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These sensors mainly take advantage of the fluorescence-quenching capability and different affinity of MoS 2 to ssDNA and ssDNA-target combination, enabling easy preparation, single-step, and rapid detection. The fluorescent donor in a MoS 2 -based FRET sensor could be dyes [73, 75-91, 95, 96, 98, 102, 104-107], carbon dots [97,100,101,108], UCNPs [109], silver NCs [99,103], QDs [52,82,92,94], and AuNCs [93]. The fluorescence emission wavelength of these donors ranges from 360 to 680 nm, which validates the broad-spectrum quenching property of MoS 2 .…”

Section: D Nanomaterials As the Acceptor In Fret Sensors 41 Mos 2 Nmentioning

confidence: 72%

“…The LOD of the MoS 2 -based assay was calculated to be 500 pm and 5 μm for DNA and adenosine, respectively. Since then, dozens of researchers have reported the development of MoS 2 -DNA-based FRET sensors for the detection of various targets, such as the nucleic acids [74][75][76][77][78][79][80][81][82][83][84][85], proteins [86][87][88][89][90][91][92][93][94][95], metal ions [96,97], small molecules [52,86,[98][99][100][101][102][103][104], enzyme activity [105], and others [106]. These sensors mainly take advantage of the fluorescence-quenching capability and different affinity of MoS 2 to ssDNA and ssDNA-target combination, enabling easy preparation, single-step, and rapid detection.…”

Section: D Nanomaterials As the Acceptor In Fret Sensors 41 Mos 2 Nmentioning

confidence: 99%

See 1 more Smart Citation

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Zhou

Chen

et al. 2020

Nanophotonics

View full text Add to dashboard Cite

Abstract Förster resonance energy transfer (FRET)–based sensing has been steadily gaining popularity in the areas of biochemical analysis, environmental monitoring, and disease diagnosis in the past 20 years. Two-dimensional (2D) nanomaterials are extensively used as donors and acceptors in the FRET sensing because of their attractive optical and chemical properties. In this review, we first present the FRET theory and calculations to give readers a better understanding of the FRET phenomenon. Then, we discuss the recent research advances in using 2D nanomaterials as donors and acceptor in FRET sensing. Finally, we summarize the existing challenges and future directions of 2D nanomaterials in the FRET sensing applications.

show abstract

Section: D Nanomaterials As the Acceptor In Fret Sensors 41 Mos 2 Nmentioning

confidence: 72%

Section: D Nanomaterials As the Acceptor In Fret Sensors 41 Mos 2 Nmentioning

confidence: 99%

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Zhou

Chen

et al. 2020

Nanophotonics

View full text Add to dashboard Cite

show abstract

“…The DNA-AgNCs were synthesized according to a previously reported synthesis method with slight modification [50], and the whole reaction was carried out in the dark. Briefly, a 100 µM DNA template was dissolved in phosphate buffer (pH = 7.2), heated at 95 • C for 10 min, and cooled in an ice bath for 10 min.…”

Section: Preparation Of Dna-agncsmentioning

confidence: 99%

A Label-Free Fluorescent Aptasensor for Detection of Staphylococcal Enterotoxin A Based on Aptamer-Functionalized Silver Nanoclusters

Zhang

Khan

et al. 2020

Polymers

Self Cite

View full text Add to dashboard Cite

Staphylococcal enterotoxin A (SEA) is a worldwide public health problem accounting for the majority of food poisoning which is produced by Staphylococcus aureus, threatening human health and leading to various foodborne diseases. Therefore, it is of great significance to develop a sensitive detection method for SEA to ensure food safety and prevent foodborne diseases in humans. In this study, an adaptive fluorescence biosensor for the detection of staphylococcal enterotoxin A (SEA) was designed and developed by combining DNA silver nanoclusters (DNA-AgNCs) with polypyrrole nanoparticles (PPyNPs). Fluorescent AgNCs, synthesized using aptamers as templates, were used as fluorescence probes, whose fluorescence was quenched by PPyNPs. In the presence of the target SEA, DNA-AgNCs were forced to desorb from the surface of PPyNPs through the binding of SEA with the aptamer-DNA-AgNCs, thereby resulting in fluorescence recovery. Under the optimized conditions, the relative fluorescence intensity (FI) showed a linear relationship with the SEA concentration in the range from 0.5 to 1000 ng/mL (Y = 1.4917X + 0.9100, R2 = 0.9948) with a limit of detection (LOD) of 0.3393 ng/mL. The sensor was successfully used to evaluate the content of SEA in milk samples, and the recovery efficiency of SEA was between 87.70% and 94.65%. Thus, the sensor shows great potential for application in food analysis. In short, the proposed platform consisted of an aptamer fluorescent sensor that can be used for the ultrasensitive detection of various toxins by taking advantage of the excellent affinity and specificity of corresponding aptamers.

show abstract

“…The design strategy for the CNP-peptide fluorescence sensor is outlined in the accompanying graphical abstract. A peptide containing six arginine residues was chosen as a sensitive trypsin substrate, because recent studies have identified that this peptide can be selectively cleaved by trypsin [ 22 , 23 ]. The surface of CNPs oxidized by nitric acid has more negatively charged groups such as COO - and OH - [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

Hou

Feng

Zhao

et al. 2020

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleave the peptide, resulting in the release of the dye moiety and a substantial increase in fluorescence intensity, which was dose- and time-dependent, and trypsin could be quantified accordingly. Correspondingly, the biosensor has led to the development of a convenient and efficient fluorescent method to measure trypsin activity, with a detection limit of 0.7 μg/mL. The method allows rapid determination of trypsin activity in the normal and acute pancreatitis range, suitable for point-of-care testing. Furthermore, the applicability of the method has been demonstrated by detecting trypsin in spiked urine samples.

show abstract

Silver nanoclusters based FRET aptasensor for sensitive and selective fluorescent detection of T-2 toxin

Cited by 77 publications

References 52 publications

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

A Label-Free Fluorescent Aptasensor for Detection of Staphylococcal Enterotoxin A Based on Aptamer-Functionalized Silver Nanoclusters

A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

Contact Info

Product

Resources

About