Silver Inlaid with Gold Nanoparticle/Chitosan Wound Dressing Enhances Antibacterial Activity and Porosity, and Promotes Wound Healing

Li, Qing; Lu, Fei; Zhou, Guofang; Yu, Kun; Lu, Bitao; Xiao, Yang; Dai, Fangyin; Deng-jun, WU; Lan, Guangqian

doi:10.1021/acs.biomac.7b01180

Cited by 157 publications

(85 citation statements)

References 45 publications

(76 reference statements)

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“…). Similar data have been recorded by Li et al ., in which these wavenumbers have been assigned to CH vibrations from the methylene group of CS. The broadening of peaks at around 3431 cm −1 wavenumber in PANI‐CS‐NC and PANI‐CS‐Ag‐NC are indicative of stretching of the carbonyl group of β‐gal, which confirms binding of the enzyme to the NCs.…”

Section: Resultssupporting

confidence: 51%

Elucidating the binding efficacy of β‐galactosidase on polyaniline–chitosan nanocomposite and polyaniline–chitosan–silver nanocomposite: activity and molecular docking insights

Khan

Husain

Ahmad

2018

J of Chemical Tech & Biotech

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BACKGROUND: Aspergillus oryzae -galactosidase ( -gal; EC 3.2.1.23) was physically adsorbed on polyaniline-chitosan nanocomposite (PANI-CS-NC) and polyaniline-chitosan-silver nanocomposite (PANI-CS-Ag-NC). Stability and kinetic studies were assessed, along with reusability and molecular docking interactions.RESULTS: According to transmission electron microscopy images, the sizes of the respective NCs were found to be in the range of 23-39 and 6-18 nm. The (effectiveness factor) values for PANI-CS-NC-and PANI-CS-Ag-NC-bound -gal were calculated to be 0.89 and 1.23, respectively, suggesting an enhancement in the activity of the enzyme in the presence of silver ions. Significant broadening was observed in pH and temperature profiles. Kinetic properties of PANI-CS-NC-and PANI-CS-Ag-NC-bound -gal showed lower K m , and two-and fourfold higher V max , respectively, compared to free enzyme. PANI-CS-Ag-NC-bound -gal was able to retain 94% of its initial activity after 10 repeated uses. Circular dichroism analysis revealed conformational changes in the secondary structure of protein upon immobilization. The genotoxic assessment revealed non-toxic behaviour of both free and enzyme-bound NC. The rate of lactose hydrolysis at 50 ∘ C exhibited by PANI-CS-Ag-NC bound -gal was 7% higher than that for the native enzyme. In-silico analysis showed efficient binding of -gal on PANI-CS-NCs due to interaction with residues that were exclusive of the active site of the enzyme. CONCLUSION: PANI-CS-Ag-NC-bound -gal preparation proved to be a robust nano-biocatalyst due to remarkable enhancement in catalytic activity along with excellent stability and reusability characteristics, which could be further utilized for efficient construction of nano-biosensors for producing lactose-free dairy products to feed lactose-intolerant patients. Lactose hydrolysis in batch processEnzymatic hydrolysis of lactose is a highly selective procedure which maintains the characteristics of a raw material in the J Chem Technol Biotechnol 2019; 94: 837-849

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Section: Resultssupporting

confidence: 51%

Elucidating the binding efficacy of β‐galactosidase on polyaniline–chitosan nanocomposite and polyaniline–chitosan–silver nanocomposite: activity and molecular docking insights

Khan

Husain

Ahmad

2018

J of Chemical Tech & Biotech

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“…These results are consistent with the antibacterial mechanism of Ag + ions that Ag + ions can induce the destruction of the bacterial membrane. [ 19 ]…”

Section: Resultsmentioning

confidence: 99%

A Macroporous Hydrogel Dressing with Enhanced Antibacterial and Anti‐Inflammatory Capabilities for Accelerated Wound Healing

Huang

Ying

Wang

et al. 2020

Adv Funct Materials

226

159

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Here, a novel macroporous hydrogel dressing is presented that can accelerate wound healing and guard against bacteria-associated wound infection. Carboxymethyl agarose (CMA) is successfully prepared from agarose. The CMA molecular chains are cross-linked by hydrogen bonding to form a supramolecular hydrogel, and the hydroxy groups in the CMA molecules complex with Ag + to promote hydrogel formation. This hydrogel composite exhibits pH-responsiveness and temperature-responsiveness and releases Ag + , an antibacterial agent, over a prolonged period of time. Moreover, this hydrogel exhibits outstanding cytocompatibility and hemocompatibility. In vitro and in vivo investigations demonstrate that the hydrogel has enhanced antibacterial and anti-inflammatory capabilities and can significantly accelerate skin tissue regeneration and wound closure. Astonishingly, the hydrogel can cause the inflammation process to occur earlier and for a shorter amount of time than in a normal process. Given its excellent antibacterial, anti-inflammatory, and physicochemical properties, the broad application of this hydrogel in bacteriaassociated wound management is anticipated.

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“…Similarly, chitosan blended with metallic nanoparticles has an eff ect on the microbial activity and wound healing [98]. Th e reductions of infl ammatory cells and mostly closed capillary lumens have been investigated by diverse research groups, using silver nanoparticles in diverse polymer matrices [89][90][91][92][93][94][95][96][97].…”

Section: Electrospinnable Polymer Materials For Wound Dressing Applicmentioning

confidence: 99%

Review of Possible Applications of Nanofibrous Mats for Wound Dressings

Mamun¹

2019

TEK

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Skin is an important part of the human body. Its function is to control the body's homeostasis mechanism. If a skin injury occurs, it is of utmost importance to heal the skin as soon as possible. The currently available medical treatment system, however, has limited eff ectiveness on the regeneration of the structure and function of the injured skin, potentially causing wound infections and dehydration. The corresponding impact on the healing process may in the worst case also be fatal. To overcome these problems in wound dressing materials, nanofi brous mats are excellent candidates for wound treatment and management. Such wound dressings can be found in biomedical applications not only in drug delivery, but also as antibacterial and antimicrobial materials, and as materials for the regeneration and repair of tissue or organs. The large surface-area-to-volume ratio is one of the unique properties of nanofi brous mats. This paper gives a brief overview of possible materials and applications of nanofi brous mats for wound dressing.

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Silver Inlaid with Gold Nanoparticle/Chitosan Wound Dressing Enhances Antibacterial Activity and Porosity, and Promotes Wound Healing

Cited by 157 publications

References 45 publications

Elucidating the binding efficacy of β‐galactosidase on polyaniline–chitosan nanocomposite and polyaniline–chitosan–silver nanocomposite: activity and molecular docking insights

Elucidating the binding efficacy of β‐galactosidase on polyaniline–chitosan nanocomposite and polyaniline–chitosan–silver nanocomposite: activity and molecular docking insights

A Macroporous Hydrogel Dressing with Enhanced Antibacterial and Anti‐Inflammatory Capabilities for Accelerated Wound Healing

Review of Possible Applications of Nanofibrous Mats for Wound Dressings

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