Silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of <i>Streptococcus sanguinis</i> and <i>Lactobacillus salivarius</i>

Godoy-Gallardo, María; Rodríguez-Hernández, Ana G.; Delgado, Luis M.; Manero, J. M.; Gil, F.J.; Rodríguez, Daniel

doi:10.1111/clr.12422

Cited by 40 publications

(52 citation statements)

References 66 publications

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“…This protocol has been previously described [11,12,16]. Briefly, functionalized and control samples were immersed in 1 ml of bacterial suspensions (1·10 8 CFU ml -1 ) for 2 hours at 37ºC.…”

Section: Bacterial Adhesion To Treated Surfacesmentioning

confidence: 99%

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Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

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Section: Bacterial Adhesion To Treated Surfacesmentioning

confidence: 99%

“…This evaluation has been detailed elsewhere [11,12,16]. Samples were immersed in 1 mL of bacterial suspension for 2 h. Next, the medium was replaced by fresh medium and bacteria were allowed to grow for 24 h at 37ºC.…”

Section: Evaluation Of Biofilm Formation Onto Treated Surfacesmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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“…Interestingly, once the percentage of inhibition was studied, the effect in S.sanguinis is correlated with the results obtained in bacterial adhesion. Each strain, however, has a distinct response to the antimicrobial surfaces, as it has been studied in previous studies 8,70 . Whereas biofilm formation of S.sanguinis was clearly higher inhibited by ATRP-coatings, no significant reduction in bacterial inhibition was observed for L.salivarius among all three strategies.…”

Section: Biological Characterization Of the Surfacesmentioning

confidence: 99%

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

et al. 2015

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Dental implant failure can be associated to infections which develop into periimplantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMP) have been studied. To covalently immobilize these molecules onto metallic substrates several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro.Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with APTES, and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the non-fouling nature of polymer PDMA segment.3

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“…However, low osteoconduction and integration of titanium-based implants with the bone for long-term survival, their weak anti-inflammatory properties, and the possibility of toxic components releasing into the human body requires surface modification and the formation of a layer, which significantly eliminates these above-mentioned adverse factors. These surface modifications can be carried out into two ways: (a) The roughness and wettability changes of the titanium implants' surface, which can stimulate a durable connection between the implant and the bone [9][10][11]; and (b) the formation of bioactive coatings, which accelerate bone formation (e.g., hydroxyapatite layers [12,13]) or increase their biocidal activity (e.g., bio-functional magnesium coating, as well as silver nanoparticles [14][15][16]). The formation of an oxide layer (passivation layer) on the surface of titanium/titanium alloy implants, which is practically insoluble and largely responsible for their high corrosion resistance and biocompatibility, is an important way to approach implants' surface modification [17].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Evaluation of the Biological Properties of Surface-Modified Titanium Alloy Implants

Piszczek

Radtke

Ehlert

et al. 2020

JCM

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An increasing interest in the fabrication of implants made of titanium and its alloys results from their capacity to be integrated into the bone system. This integration is facilitated by different modifications of the implant surface. Here, we assessed the bioactivity of amorphous titania nanoporous and nanotubular coatings (TNTs), produced by electrochemical oxidation of Ti6Al4V orthopedic implants' surface. The chemical composition and microstructure of TNT layers was analyzed by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). To increase their antimicrobial activity, TNT coatings were enriched with silver nanoparticles (AgNPs) with the chemical vapor deposition (CVD) method and tested against various bacterial and fungal strains for their ability to form a biofilm. The biointegrity and anti-inflammatory properties of these layers were assessed with the use of fibroblast, osteoblast, and macrophage cell lines. To assess and exclude potential genotoxicity issues of the fabricated systems, a mutation reversal test was performed (Ames Assay MPF, OECD TG 471), showing that none of the TNT coatings released mutagenic substances in long-term incubation experiments. The thorough analysis performed in this study indicates that the TNT5 and TNT5/AgNPs coatings (TNT5-the layer obtained upon applying a 5 V potential) present the most suitable physicochemical and biological properties for their potential use in the fabrication of implants for orthopedics. For this reason, their mechanical properties were measured to obtain full system characteristics.

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Silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of Streptococcus sanguinis and Lactobacillus salivarius

Abstract: Silver deposition on titanium with a novel electrochemical anodizing process produced surfaces with significant antibacterial properties in vitro without negative effects on cell viability.

Cited by 40 publications

References 66 publications

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

Comprehensive Evaluation of the Biological Properties of Surface-Modified Titanium Alloy Implants

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