Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Abstract

Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

Single-step pulsed electrodeposition of calcium phosphate coatings on titanium for drug delivery

“…Polished samples were ultrasonically cleaned in acetone, ethanol and ultrapure water. Before electrodeposition, samples were treated in NaOH 5 M solution for 24 h at 60°C [33], rinsed with ultrapure water and dried in a desiccator.…”

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

“…cell adhesion ability, onto the substrate [166]. Very recently, Godoy-Gallardo et al [167] have shown certain silanes could induce osteoblast differentiation but reduce bacterial and biofilm adhesion on titanium surface. Besides the cell signalling ability, silane is thin and strong that even remain after the resin debonding.…”

Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants

“…This effect can be further improved by using silanes that harbour biological activities themselves. For instance, the silane triethoxysilylpropyl succinic anhydride (TESPSA) has been shown to present both osteoinductive and antibacterial activity when immobilized on titanium surfaces [30,31]. Importantly, the biological activity of the silane and its straightforward chemistry allows for short and simple protocols, rendering this type of functionalization especially feasible for applications in the dental industry.…”