Siloxane-poly(lactic acid)-vaterite composites with 3D cotton-like structure

Abstract

Trace amounts of ionic calcium and silicon species have been reported to stimulate the proliferation, differentiation, and mineralization of bone-forming cells. Composite materials comprising siloxane-doped calcium carbonate (vaterite) particles and poly(L-lactic acid) have been developed [siloxane-poly(lactic acid)-vaterite hybrid-composite, SiPVH] so far; they were designed such that calcium and silicate ions are gradually released from SiPVH and they show the chronic effects of ions on cellular activities. In the present work, SiPVH with a 3D cotton-like structure was prepared by electrospinning to obtain the major advantages of excellent bioactivity and ease of handling for bone filling surgery. The diameter of the fibrous skeletons that form structure of the cotton-like SiPVH was controlled to ~10 μm to achieve cellular migration into the spaces between fibers. The resulting cotton-like SiPVH showed good flexibility. The fiber surface was coated rapidly with numerous particles of several hundred nanometers in size by alternate soaking in CaCl(2) and Na(2)HPO(4). The treated cotton-like material, which released calcium and silicate ions gradually, showed good cellular migration behavior into the 3D structure in cell culture tests using murine osteoblast-like MC3T3-E1 cells.

Preparation of Cotton-Wool-Like Poly(lactic acid)-Based Composites Consisting of Core-Shell-Type Fibers

“…The preparation method of cotton-wool-like materials has been reported in our earlier work [16]. After the electrospun fibers fly to rush in ethanol, the electrical charge is neutralized instantly.…”

“…Although nonwoven materials are very promising for bone regeneration, their thickness is usually limited to ~0.1–0.2 mm. In our earlier work, cotton-wool-like SiVPCs were developed using a modified electrospinning method [16]. From the viewpoint of the significance for in vitro bioactivity and animal tests, cotton-wool-like SiVPCs containing 47 vol % of SiV are expected to be one of the best-performing materials.…”

Combinatorial effects of inorganic ions on adhesion and proliferation of osteoblast‐like cells

“…Reportedly, the proliferation and differentiation of osteoblast‐like cells is more effectively promoted by SiV/PLLA composites than by vaterite/PLLA composites, which release no Si ions. Electrospun SiV/PLLA composites have a cotton wool‐like structure with high porosity, which helps achieve good cell migration into the inside of the materials, and high flexibility, which allows ease of handling . Aminopropyltriethoxysilane (APTES) is the Si source used in SiV, and the Si ions released by SiV have a structure different from that of Si ions released by conventional bioactive glasses, such as 45S5 bioglass.…”

“…Electrospun SiV/PLLA composites have a cotton wool-like structure with high porosity, which helps achieve good cell migration into the inside of the materials, and high flexibility, which allows ease of handling. [11][12][13] Aminopropyltriethoxysilane (APTES) is the Si source used in SiV, and the Si ions released by SiV have a structure different from that of Si ions released by conventional bioactive glasses, such as 45S5 bioglass. Although osteoblast-like cells have demonstrated differences between the responses to Si ions released from SiV and 45S5 bioglass as well as different Si dose dependence, Si ions from both sources enhance cell mineralization.…”

Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite

“…Composite materials consisting of SiV and biodegradable polymers, such as poly(L-lactic acid) (PLLA) and poly(lactic-co-glycolic acid), were developed as fibre mats and cotton wool-like structures by electrospinning [14][15][16][17]. Proliferation and bonelike properties of mouse osteoblast-like cells (MC3T3-E1 cells) were enhanced to a larger extent on SiV-PLLA composites (releasing both Si and Ca) than on vaterite-PLLA composites (releasing only Ca) [14].…”