Combinatorial effects of inorganic ions on adhesion and proliferation of osteoblast‐like cells

Abstract

Combinatorial effects of three ions, namely silicate (Si), calcium (Ca), and magnesium (Mg) ions, on the adhesion and proliferation of MC3T3‐E1 mouse osteoblast‐like cells were evaluated. The cells were cultured in single‐, dual‐, or triple‐ion‐conditioned culture media with systematically changed ion concentrations. The ranges of Si, Ca, and Mg ion concentrations were set as 10–70, 80–400, and 25–500 ppm, respectively. The numbers of adherent live cells were measured after culturing for 3 h and for 1, 3, and 5 days to examine cell adhesion and proliferation, respectively. Mg ions predominantly enhanced cell adhesion in both the dual‐ion (xSi‐zMg and yCa‐zMg) and triple‐ion (xSi‐yCa‐zMg) systems but had no effect when they acted individually in the single‐ion system. Conversely, Si ions predominantly enhanced cell proliferation in most single‐ and triple‐ion‐conditioned media. Evaluation of the combinatorial effects of the three ions on cell adhesion and proliferation revealed that the dual‐ and triple‐ion‐conditioned media mainly conferred synergistic effects on adhesion but antagonistic effects on proliferation. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1042–1051, 2019.

A ternary nanocomposite fibrous scaffold composed of poly(ε‐caprolactone)/Gelatin/Gehlenite (Ca2Al2SiO7): Physical, chemical, and biological properties in vitro

“…However, during the rest of culture time, the PCL/GT and PCL/GT/GLN7% scaffolds' values overtook the PCL scaffold and even the control showing their great potential to provide a more suitable environment for the cells growth and proliferation. It is noteworthy that the scaffold containing the GLN nanoparticles exhibited more cells growth than the one without the nanoparticles rooting in the stimulating effects of GLN's ions like Ca and Si; these ions were reported to enhance osteoblast cells proliferation and differentiation 54,55 . The cell viability results showed that the ternary scaffold had greater potential for the cells growth than other scaffolds here.…”

“…However, at day 14, the PCL/GT/GLN7% scaffold had a higher ALP activity than other scaffolds and this trend was again repeated at the last time interval. The reason behind superiority of PCL/GT/GLN7% scaffold can be traced through the stimulating effects of Ca and Si ions on the osteoblast cells activity 54,55 …”

Osteogenic Capability of Vaterite‐Coated Nonwoven Polycaprolactone Scaffolds for In Vivo Bone Tissue Regeneration

“…The concentration of extracellular Ca 2+ cations is a key factor for osteoblasts mineralization activity because the Ca 2+ is closely involved in the regulation of integrins ligand-binding processes and therefore mediation of adhesion, proliferation, and differentiation of cells in a matrix. [57] It was revealed that the increase of local concentrations of Ca 2+ results in enhanced osteoblasts adhesion, proliferation, and differentiation, which should promote their osteogenic activity. [58,59] Also, the vaterite can serve as a vehicle for bioactive species due to its high degree of porosity.…”

Fabrication of physical and chemical crosslinked hydrogels for bone tissue engineering

“…Recent studies also proved that Mg 2+ with an appropriate concentration (50–200 ppm) can observably promote osteogenic differentiation and considerable new bone regeneration in bone defects [ 36 , 37 ]. Based on the above facts, Pan et al designed a macro-porous structured GelMA hydrogel with the incorporation of MgO nanoparticles (NPs) via thiol-ene click reactions [ 38 ].…”