Silk fibroin/sericin 3D sponges: The effect of sericin on structural and biological properties of fibroin

Siavashani, Abdollah Zakeri; Mohammadi, Javad; Rottmar, Markus; Şentürk, Berna; Nourmohammadi, Jhamak; Sadeghi, Behnam; Huber, Lukas A.; Maniura‐Weber, Katharina

doi:10.1016/j.ijbiomac.2020.02.316

Cited by 44 publications

(25 citation statements)

References 88 publications

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“…[80] Interestingly, biomaterials prepared from either SS or SF were shown to be immunoinert or low immunogenic in several studies targeting different applications, [81] while the mixed SP-based materials activated immune response by a mechanism, which is still unclear. [10,21,82] It was indeed suggested in some cases that SS is the immunotoxic element of SP, which was used to justify cocoon degumming and research projects focusing on purified SF. [10,65] Conversely, it was shown in other research focusing on SS that it is a low immunogenic material with anti-inflammatory properties.…”

Section: Immune Response To Silk Sericin-silk Fibroin Compositesmentioning

confidence: 99%

“…[10,21,82] It was indeed suggested in some cases that SS is the immunotoxic element of SP, which was used to justify cocoon degumming and research projects focusing on purified SF. [10,65] Conversely, it was shown in other research focusing on SS that it is a low immunogenic material with anti-inflammatory properties. [34,46] While the constitutive element of SP triggering the immune reaction remains unknown, it has been suggested that the response could be due to the presence of 𝛽-sheet domains, which are hydrophobic.…”

Section: Immune Response To Silk Sericin-silk Fibroin Compositesmentioning

confidence: 99%

“…As pointed out in Section 2.2, SS improves the mechanical properties of SF via enhanced 𝛽-sheet formation. [10] Consequently, SS-SF composites could constitute good bone scaffold biomaterials, exhibiting suitable mechanical properties and stimulating osteoinduction. For example, it has been reported that SS-SF 3D sponges exhibited excellent osteoblast-like MG63 cell attachment without excessive immune response.…”

Section: Bone Biomaterialsmentioning

confidence: 99%

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Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Boni

Bakadia

Osi

et al. 2021

Macromolecular Bioscience

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The unique properties of silk proteins (SPs), particularly silk sericin (SS) and silk fibroin (SF), have attracted attention in the design of scaffolds for tissue engineering over the past decades. Since SF has good mechanical properties, while SS displays bioactivity, scaffolds combining both proteins should exhibit complementary properties enhancing the potential of these materials. Unfortunately, SS-SF composites can generate chronic immune responses and their immunogenic element is not completely clear. The potential of SS-SF composites in tissue engineering, elements which may contribute to their immunogenicity, and alternatives for their preparation and design, to modulate the immune response and take advantage of their useful properties, are discussed in this review. It is known that SS can enhance 𝜷-sheet formation in SF, which may act as hydrophobic regions with a strong affinity for adsorption proteins inducing the chronic recruitment of inflammatory cells. Therefore, tailoring the exposure of hydrophobic regions at the scaffold surface should represent a viable strategy to modulate the immune response. This can be achieved by coating SS-SF composites with SS or other hydrophilic polymers, to take advantage of their antibiofouling properties. Research is still needed to realize the full potential of these composites for tissue engineering.

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Section: Immune Response To Silk Sericin-silk Fibroin Compositesmentioning

confidence: 99%

Section: Immune Response To Silk Sericin-silk Fibroin Compositesmentioning

confidence: 99%

Section: Bone Biomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Boni

Bakadia

Osi

et al. 2021

Macromolecular Bioscience

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“…After dissolution, the remnants of chemicals were removed through dialysis with a cellulose dialysis membrane in distilled water for 3 days. After dialysis the silk fibroin solution was sonicated at 20 kHz: 400W for 1 hr and then lyophilized to obtain silk fibroin powder (Ha, Park & Hudson, 2003;Siavashani et al, 2020).…”

Section: Dialysismentioning

confidence: 99%

Silk derived formulations for accelerated wound healing in diabetic mice

Tariq¹,

Tahir²,

Butt³

et al. 2021

PeerJ

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Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

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“…[ 63 ] In addition, the incorporation of functional molecules or agents in scaffolds to modulate recruited macrophages has also drawn the interest of researchers. Biomolecules such as 25‐hydroxyvitamin D3, [ 64 ] SEW2871, [ 65 ] aspirin‐triggered resolvin D1, [ 66 ] polymerizable fucoidan and carrageenans, [ 67 ] lanthanum, [ 68 ] and sericin [ 69 ] capable of promoting M2 macrophage polarization or anti‐inflammatory processes were applied and investigated. Surface coatings for immune evasion have been widely investigated on synthetic material‐derived grafts as stealth coatings.…”

Section: Immunocompatibility Of Engineered Tissue or Organ‐based Implantsmentioning

confidence: 99%

Tissue Engineering for Mimics and Modulations of Immune Functions

Tao

Wang

2021

Adv Healthcare Materials

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In the field of regenerative medicine, advances in tissue engineering have surpassed the reconstruction of individual tissues or organs and begun to work towards engineering systemic factors such as immune objects and functions. The immune system plays a crucial role in protecting and regulating systemic functions in the human body. Engineered immune tissues and organs have shown potential in recovering dysfunctions and aplasia of the immune system and the evasion from immune‐mediated inflammatory responses and rejection elicited by engineered implants from allogeneic or xenogeneic sources are also being pursued to facilitate clinical transplantation of tissue engineered grafts. Here, current progress in tissue engineering to mimic or modulate immune functions is reviewed and elaborated from two perspectives: 1) engineering of immune tissues and organs per se and 2) immune evasion of host immunoinflammatory rejection by tissue‐engineered implants.

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Silk fibroin/sericin 3D sponges: The effect of sericin on structural and biological properties of fibroin

Cited by 44 publications

References 88 publications

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Silk derived formulations for accelerated wound healing in diabetic mice

Tissue Engineering for Mimics and Modulations of Immune Functions

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