Silk fibroin/alumina nanoparticle scaffold using for osteogenic differentiation of rabbit adipose-derived stem cells

Zafar, Bahar; Mottaghitalab, Fatemeh; Shahosseini, Zahra; Negahdari, Babak; Farokhi, Mehdi

doi:10.1016/j.mtla.2019.100518

“…Silk protein generated from silk cocoons has been extensively used for the medical applications including wound healing, tissue regeneration and drug delivery [5,10,22]. Silk derivatives contain high quantity of (Gly-Ala-Gly-Ser)n sequences in the crystalline region, (Ala)n sequences as well as Arg-Gly-Asp tripeptide, which may function as a biological recognition signal and encourage cell adhesion [21][22][23]. Besides, Ch derivative as a biomimetic polymer owing to its hemostatic, stimulation of healing, antimicrobial, nontoxic, biocompatible and biodegradable properties has received signi cant status on wound healing and skin tissue regeneration [5,6,19].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Fathi

¹

,

Khanmohammadi

²

,

Goodarzi

³

et al. 2020

Preprint

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Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

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“…Ch (low in molecular weight (Mw), degree of deacetylation 75-85%) was obtained from Shanghai Hanshare Industry Co., Ltd. Silk was obtained by following previous reported protocols [19]. Brie y, cocoons of mulberry Bombyx mori silkworm were heated in 0.5% (w/v) sodium carbonate within 1 h followed by washing in distilled water through centrifugation at 4000 rpm for 10 min which help to remove debris.…”

Section: Methodsmentioning

confidence: 99%

“…Silk protein generated from silk cocoons has been extensively used for the medical applications including wound healing, tissue regeneration and drug delivery [4,7,19]. Besides, Ch derivative as a biomimetic polymer has antibacterial effects on wound healing and skin tissue regeneration [4,5,16].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Chitosan-Polyvinyl Alcohol and Silk Electrospun Fiber Seeded with Differentiated Keratinocyte for Skin Tissue Regeneration in Animal Wound Model

Fathi

¹

,

Khanmohammadi

²

,

Goodarzi

³

et al. 2020

Preprint

1

0

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Abstract Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. The fabricated fibrous Ch-PVA + Silk hydrogels were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mats increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

show abstract

“…Silk protein generated from silk cocoons has been extensively used for the medical applications including wound healing, tissue regeneration and drug delivery [4,7,19]. Silk derivatives contain high quantity of (Gly-Ala-Gly-Ser)n sequences in the crystalline region, (Ala)n sequences as well as Arg-Gly-Asp tripeptide, which may function as a biological recognition signal and encourage cell adhesion [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…Silk protein generated from silk cocoons has been extensively used for the medical applications including wound healing, tissue regeneration and drug delivery [4,7,19]. Silk derivatives contain high quantity of (Gly-Ala-Gly-Ser)n sequences in the crystalline region, (Ala)n sequences as well as Arg-Gly-Asp tripeptide, which may function as a biological recognition signal and encourage cell adhesion [18][19][20]. Besides, Ch derivative as a biomimetic polymer owing to its hemostatic, stimulation of healing, antimicrobial, nontoxic, biocompatible and biodegradable properties has received signi cant status on wound healing and skin tissue regeneration [4,5,16].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Fathi

¹

,

Khanmohammadi

²

,

Goodarzi

³

et al. 2020

Preprint

View full text Add to dashboard Cite

Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

show abstract

Silk fibroin/alumina nanoparticle scaffold using for osteogenic differentiation of rabbit adipose-derived stem cells

Cited by 30 publications

References 48 publications

Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Fabrication of Chitosan-Polyvinyl Alcohol and Silk Electrospun Fiber Seeded with Differentiated Keratinocyte for Skin Tissue Regeneration in Animal Wound Model

Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

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