Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Abstract

Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat

“…Among biomaterials, hybrid of PVA and Col derivatives has been used in both skin and nerve engineering. [22][23][24][25] However, most of them are crosslinked through hydrogen bonds, UV-radiation, glutaraldehyde and carbodiimide. In this study, we have attempted to develop fabrication of PVA and Col fibrous mat as a biological response dressing patch.…”

Production of Cell Enclosing Silk Derivative Microsphere in Uniform Size Distribution Through Coaxial Microfluidic Device and Horseradish Crosslinking Reaction

“…The SF is one of the valuable and applicable natural polymers for its high strength (1, 7), good biological compatibility, and low immunogenicity that resulted in a wide application for various biomedical purposes (4-6). It can be physically and chemically modi ed to amend the biophysical and biochemical properties of SF-based scaffolds and constructs for tissue engineering and drug delivery purposes (1,10). SF-based constructs can be fabricated through different techniques and shapes of architectures such as lms, sponges, nano/micro-particles, bers/tubes, bulk hydrogel, and conduit (1,8,10).…”

“…It can be physically and chemically modi ed to amend the biophysical and biochemical properties of SF-based scaffolds and constructs for tissue engineering and drug delivery purposes (1,10). SF-based constructs can be fabricated through different techniques and shapes of architectures such as lms, sponges, nano/micro-particles, bers/tubes, bulk hydrogel, and conduit (1,8,10).…”

“…indicated that cell mitochondrial activity elevated until the 18 days of microencapsulation, which can be attested to denser microstructures (46). The cellular growth and proliferation in SF-Ph microparticles would be due to the existence of cell interactive moieties in SF structure, such as cell adhesive motifs (10,47). Meanwhile, cellular growth on hydrogel surfaces for most polysaccharides and synthetic polymers hindered and obtained a low proliferation rate.…”

“…Hierarchical self-assembly of SF structure in nature is composed of amorphous and crystalline domains, imparting high toughness and strength to silk, leading to unique mechanical properties (1,7). Owing to these, SF has found its way in many elds, including sustained drug delivery, biosensing, adhesives, wound repair, tissue engineering in several forms: nano bers, membranes, scaffolds, hydrogels, microstructures (1,4,(8)(9)(10). The SF utilized in tissue engineering is mainly modi ed and crosslinked to form a three-dimensional (3D) shape (11).…”