Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat

Abstract

Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration. Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch. The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes. The EGCG as anti-oxidative/inflammatory substrate was encapsulated efficiently in NCs and released in a sustained manner. The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model. Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG. The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity. Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples. The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.

Biomedical properties and hemostatic efficacy of polyvinyl alcohol (PVA) based hydrogel in experimental rat liver injury model

“…3,31 Contrary to this, various studies demonstrated the positive role of PVA in biomedical uses, benefits of PVA such as biocompatibility, anti-inflammatory and antimicrobial properties are consequential and cannot be ignored even if PVA shows less efficacy than other hemostatic agents. 32,33 In our study in the same way with previous studies, no cytotoxic effects of PVA were observed. And it can be a great advantage for using this material despite the unproven powerful effect.…”

Fabrication of alginate‐based hydrogel microparticle via ruthenium‐catalyzed photocrosslinking

“…The ADSCs at 2 × 10 4 cells/well were seeded on hydrogel surfaces. The morphology, viability, and growth ability of seeded cells were monitored by microscopic observation, trypan blue exclusion dye assay, and water‐soluble formazan dye assay 3,4,8 …”

“…[1][2][3][4][5][6][7] These hydrogels are formed in different geometries, such as nanoparticle, microparticle, microcapsules, and microfibers. 3,4,8 Among these, the microparticles are commonly crosslinked physically through thermo-crosslinking and ionic-crosslinking strategies and chemically through photoinitiated or enzymatic polymerization approaches. 2,4,9 The physically crosslinked microparticles are easily undergo decomposition and swelling under physiological conditions and subsequently, their long-term mechanical stability is insufficient for cell or drug delivery applications.…”

“…Subsieve‐size injectable hydrogels with the ability to support cells mechanically and biologically are promising candidates for cell or bioactive molecule delivery applications in vivo 1–7 . These hydrogels are formed in different geometries, such as nanoparticle, microparticle, microcapsules, and microfibers 3,4,8 . Among these, the microparticles are commonly crosslinked physically through thermo‐crosslinking and ionic‐crosslinking strategies and chemically through photoinitiated or enzymatic polymerization approaches 2,4,9 .…”

Effectiveness of insolubilized polyvinyl alcohol-based electrospun fibers loaded methylprednisolone by enzyme-catalyzed cross-linking in spinal cord regeneration

“…Appropriate tissue scaffolds have to provide biologically interactive microenvironment, mechanically durable and elasticized structure like native nerve tissue, and topographically biomimetic architecture, which would be feasible through combination of synthetic and natural biopolymers, since each one possesses speci c required properties [11][12][13][14]. Besides, the electrospinning technique has been used for manufacturing brous scaffolds composed of biopolymers and bioactive molecules, which is a favorable microenvironment from structural point of view for cell culture, drug loading, and tissue regeneration because of their similarity to the natural ECM architecture and their high surface-to-volume ratio [15][16][17].…”