Silk based scaffolds with immunomodulatory capacity: anti-inflammatory effects of nicotinic acid

Siavashani, Abdollah Zakeri; Mohammadi, Javad; Maniura‐Weber, Katharina; Şentürk, Berna; Nourmohammadi, Jhamak; Sadeghi, Behnam; Huber, Lukas A.; Rottmar, Markus

doi:10.1039/c9bm00814d

Cited by 18 publications

(9 citation statements)

References 99 publications

(106 reference statements)

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“…To improve the retention time of exosomes in vivo , we introduced hydrogel formed by SS and SF. It has been found that undegummed native silk can induce a severe immunoglobulin E mediated allergy or inflammation, delaying the wound healing process ( 46 ). However, there are some conflicting reports regarding the possible immunogenicity of silk proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, SS has been used to treat myocardial infarction ( 49 ), skin wound ( 50 ), and bone injury ( 51 – 53 ). During the purification of silk sericin, the majority of small molecule impurities such as waxes, sugars and fats are expected to be removed during dialysis ( 46 , 54 ), which may indicate that impurities in sericin is the cause for immune response. Another study showed that synergistic effect of crystalline fibroin coated with sericin protein and lipopolysaccharides, trigging significant release of TNF-α from macrophages, which indicates that sericin mediated inflammation activation is dependent on the association with core fibroin fibers ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

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Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

Han

Liu

Chen

et al. 2021

Front. Cardiovasc. Med.

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Recent studies have shown that the hydrogels formed by composite biomaterials are better choice than hydrogels formed by single biomaterial for tissue repair. We explored the feasibility of the composite hydrogel formed by silk fibroin (SF) and silk sericin (SS) in tissue repair for the excellent mechanical properties of SF, and cell adhesion and biocompatible properties of SS. In our study, the SF SS hydrogel was formed by SF and SS protein with separate extraction method (LiBr dissolution for SF and hot alkaline water dissolution for SS), while SF-SS hydrogel was formed by SF and SS protein using simultaneous extraction method (LiBr dissolution for SF and SS protein). The effects of the two composite hydrogels on the release of inflammatory cytokines from macrophages and the wound were analyzed. Moreover, two hydrogels were used to encapsulate and deliver human umbilical cord mesenchymal stem cell derived exosomes (UMSC-Exo). Both SF SS and SF-SS hydrogels promoted wound healing, angiogenesis, and reduced inflammation and TNF-α secretion by macrophages. These beneficial effects were more significant in the experimental group treated by UMSC-Exo encapsulated in SF-SS hydrogel. Our study found that SF-SS hydrogel could be used as an excellent alternative to deliver exosomes for tissue repair.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

Han

Liu

Chen

et al. 2021

Front. Cardiovasc. Med.

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“…[ 238 ] 3D silk scaffolds prepared by freeze‐drying afforded them high porosity and well‐connected macropores. [ 239 ] Incorporation of nicotinic acid into silk scaffolds showed a sustained drug release and suppressed gene expression of pro‐inflammatory markers TNF‐ α , CXCL10, and CD197 from macrophages. A silk hydrogel system formed by simply blending mulberry and non‐mulberry silk.…”

Section: Strategies To Regulate Immune Responsementioning

confidence: 99%

Toward a Better Regeneration through Implant‐Mediated Immunomodulation: Harnessing the Immune Responses

Zhang

Zhou

et al. 2021

Advanced Science

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Tissue repair/regeneration, after implantation or injury, involves comprehensive physiological processes wherein immune responses play a crucial role to enable tissue restoration, amidst the immune cells early‐stage response to tissue damages. These cells break down extracellular matrix, clear debris, and secret cytokines to orchestrate regeneration. However, the immune response can also lead to abnormal tissue healing or scar formation if not well directed. This review first introduces the general immune response post injury, with focus on the major immune cells including neutrophils, macrophages, and T cells. Next, a variety of implant‐mediated immunomodulation strategies to regulate immune response through physical, chemical, and biological cues are discussed. At last, various scaffold‐facilitated regenerations of different tissue types, such as, bone, cartilage, blood vessel, and nerve system, by harnessing the immunomodulation are presented. Therefore, the most recent data in biomaterials and immunomodulation is presented here in a bid to shape expert perspectives, inspire researchers to go in new directions, and drive development of future strategies focusing on targeted, sequential, and dynamic immunomodulation elicited by implants.

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“…The stress–strain curves of materials can be distinctly different. For example, the stress–strain curves of human skin, [ 31 ] silk hydrogel, [ 32 ] and silicone foam composite [ 33 ] are monotonically concave (J‐shaped); the stress–strain curves of concrete, [ 34 ] ceramic, [ 35 ] and silk scaffold [ 36 ] are monotonically convex; the stress–strain curves of aluminum foam [ 37 ] and rubber composites [ 38 ] have a plateau regime. To verify the practicality of the three simple schemes proposed in Section 4, the stress–strain curves of silicone foam composite, aluminum foam, and silk scaffold are set as target curves, respectively (Figure 5b–d).…”

Section: Validation Of the Strategymentioning

confidence: 99%

A Stair‐Building Strategy for Tailoring Mechanical Behavior of Re‐Customizable Metamaterials

Lin

Pan

Yang

et al. 2021

Adv Funct Materials

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Re‐customizable mechanical behavior is critical for versatile materials with tunable functions and applications, but inverse design for varying targets is often hindered by complex coupling between structural topologies and mechanics. In this work, a novel “stair‐building” strategy for customizing as well as re‐customizing target mechanical behavior for mechanical metamaterials is proposed. Similar to building a stair with bricks, customizing or re‐customizing a target stress–strain (force–displacement) curve for the material can be realized by stacking the brick‐like loading curves of bistable units visually. The mechanical feasibility of the “stair‐building” strategy is firstly physically realized in a type of array‐structured multistable mechanical metamaterial and then carefully verified by theoretical mechanics analysis. Accordingly, three specific simple design schemes are further proposed for implementation. The “stair‐building” strategy is proved to be rapid, effective, and accurate for mechanical behavior customization by both experiments and finite element simulations. Moreover, re‐customization for diverse mechanical behaviors in a wide range can be realized by the same piece of metamaterial. This design strategy provides a novel approach for tailoring metamaterials with re‐customizable target mechanical behaviors and applies to a variety of bistable units.

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Silk based scaffolds with immunomodulatory capacity: anti-inflammatory effects of nicotinic acid

Abstract: Here we show that 3D silk scaffolds loaded with nicotinic acid have great potential for tissue engineering due to their excellent cytocompatibility and ability to decrease the expression of proinflammatory markers in a concentration dependent manner.

Cited by 18 publications

References 99 publications

Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

Toward a Better Regeneration through Implant‐Mediated Immunomodulation: Harnessing the Immune Responses

A Stair‐Building Strategy for Tailoring Mechanical Behavior of Re‐Customizable Metamaterials

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