Silicon microneedle array for minimally invasive human health monitoring

Smith, Robert L.; Collins, Scott D.; Duy, Janice; Minogue, Timothy D.

doi:10.1117/12.2299264

Cited by 8 publications

(11 citation statements)

References 19 publications

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“…So far, MN were at best used for sampling and releasing total skin ISF and circulating nucleic acids detected after heavy sample processing and PCR-based analysis. 37…”

mentioning

confidence: 99%

“…In addition, to the best of our knowledge, there has been no report of MNs engineered to sample and detect specific nucleic acid biomarkers from skin ISF. So far, MNs were at best used for sampling and releasing total skin ISF and circulating nucleic acids detected after heavy sample processing and PCR-based analysis …”

mentioning

confidence: 99%

“…So far, MNs were at best used for sampling and releasing total skin ISF and circulating nucleic acids detected after heavy sample processing and PCR-based analysis. 37 Herein, we report on the engineering of hydrogel-coated MN patches that can sample and isolate specific miRNA biomarkers from skin ISF at the fastest rate, while enabling the captured miRNA to be detected in situ (Figure 1a). Our versatile platform also offers the capability of light-triggered release of the miRNA for post-sampling off-chip analysis.…”

mentioning

confidence: 99%

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Hydrogel-Coated Microneedle Arrays for Minimally Invasive Sampling and Sensing of Specific Circulating Nucleic Acids from Skin Interstitial Fluid

et al. 2019

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Minimally-invasive technologies that can sample and detect cell-free nucleic acid biomarkers from liquid biopsies have recently emerged as clinically useful for early diagnosis of a broad range of pathologies, including cancer. Although blood has been so far the most commonly interrogated body fluid, skin interstitial fluid has been mostly overlooked despite containing the same broad variety of molecular biomarkers originating from cells and surrounding blood capillaries. Minimally-invasive technologies have emerged as a method to sample this fluid in a pain-free manner and often take the form of microneedle patches. Herein, we developed microneedles that are coated with an alginate-peptide nucleic acid hybrid material for sequence-specific sampling, isolation and detection of nucleic acid biomarkers from skin interstitial fluid. Characterized by fast sampling kinetics and large sampling capacity (~6.5μL in 2 min), this platform technology also enables for the first time the detection of specific nucleic acid biomarkers either on the patch itself or in solution after light-triggered release from the hydrogel. Considering the emergence of cellfree nucleic acids in bodily fluids as clinically informative biomarkers, platform technologies that can detect them in an automated and minimally invasive fashion have great potential for personalized diagnosis and longitudinal monitoring of patient-specific disease progression.

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“…So far, MN were at best used for sampling and releasing total skin ISF and circulating nucleic acids detected after heavy sample processing and PCR-based analysis. 37…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Hydrogel-Coated Microneedle Arrays for Minimally Invasive Sampling and Sensing of Specific Circulating Nucleic Acids from Skin Interstitial Fluid

et al. 2019

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“…The result demonstrated the identification of potential disease biomarkers which is only achievable using the traditional blood sampling method. [ 148 ]…”

Section: Biosensors—sensing and Sampling Isfmentioning

confidence: 99%

Sampling Dermal Interstitial Fluid Using Microneedles: A Review of Recent Developments in Sampling Methods and Microneedle‐Based Biosensors

Saifullah

Rad

2023

Adv Materials Inter

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“…Biofluid uptake through the orifice into the hollow microneedle shaft could occur by capillary action, or biofluid flow could be induced through a microfluidic system, usually by applying negative pressure [37]- [42]. This technique has been used to extract dermal ISF using hollow silicon microneedles for on-chip detection (see "Microneedles for in situ biosensing," below), as well as offchip nucleic acid profiling to identify potential disease biomarkers, in conjunction with conventional sample processing techniques and PCR-based analysis [43]. One study reported an ISF uptake rate of 1 µL/s [39].…”

Section: Microneedles For Biofluid Extractionmentioning

confidence: 99%

The diagnostic potential of microneedles in infectious diseases

Dixon

Lau

Moghimi

et al. 2020

Precision Nanomedicine

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The coronavirus disease 2019 (COVID-19) pandemic has taught us much about our weaknesses in the management of infectious disease outbreaks. A key lesson has been the need for more effective pointof-care diagnostic tools that produce not only rapid and reliable results but also facilitate decentralised testing to avoid overwhelming central test facilities when demand peaks in an outbreak. Microneedle devices can be inserted painlessly into the skin to detect biomolecules in the epidermal and dermal layers. They have been used to identify biomarkers in both the interstitial fluid and capillary blood. Importantly, they are amenable to self-administration. In this article, we provide an overview of existing microneedle-based diagnostic technologies and discuss how they may be built upon to provide effective diagnostic tools for infectious diseases.

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Silicon microneedle array for minimally invasive human health monitoring

Cited by 8 publications

References 19 publications

Hydrogel-Coated Microneedle Arrays for Minimally Invasive Sampling and Sensing of Specific Circulating Nucleic Acids from Skin Interstitial Fluid

Hydrogel-Coated Microneedle Arrays for Minimally Invasive Sampling and Sensing of Specific Circulating Nucleic Acids from Skin Interstitial Fluid

Sampling Dermal Interstitial Fluid Using Microneedles: A Review of Recent Developments in Sampling Methods and Microneedle‐Based Biosensors

The diagnostic potential of microneedles in infectious diseases

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