Silicon chips detect intracellular pressure changes in living cells

Gómez-Martínez, Rodrigo; Hernández‐Pinto, Alberto M.; Duch, Marta; Vázquez, Patricia; Zinoviev, Kirill; Rosa, Enrique J. de la; Estéve, J.; Suárez, Teresa; Plaza, J.A.

doi:10.1038/nnano.2013.118

Cited by 70 publications

(66 citation statements)

References 29 publications

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“…In cell culture, FRET has also been used to measure uniaxial compression (Paszek et al, 2014). More generally, fluorescent materials exhibit a variety of properties that could be dependent on other mechanical quantities, such as intracellular pressure (Gomez-Martinez et al, 2013;Watanabe et al, 2013), and thus could be used to develop new force probes.…”

Section: Fretmentioning

confidence: 99%

Measuring forces and stressesin situin living tissues

2016

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Development, homeostasis and regeneration of tissues result from a complex combination of genetics and mechanics, and progresses in the former have been quicker than in the latter. Measurements of in situ forces and stresses appear to be increasingly important to delineate the role of mechanics in development. We review here several emerging techniques: contact manipulation, manipulation using light, visual sensors, and non-mechanical observation techniques. We compare their fields of applications, their advantages and limitations, and their validations. These techniques complement measurements of deformations and of mechanical properties. We argue that such approaches could have a significant impact on our understanding of the development of living tissues in the near future.

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Section: Fretmentioning

confidence: 99%

Measuring forces and stressesin situin living tissues

2016

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“…The two opposite surfaces of the cantilever act as mirrors that enhance the light reflectivity for certain wavelengths in which constructive interference occurs between the multiple reflections from the two mirrors. Conversely, the reflectivity is suppressed for other wavelengths by destructive interference 31,32 . This is illustrated in the modulation of the reflectivity with the wavelength observed in the cantilever with respect to the preclamping, in which interference effects are negligible (Fig.…”

Section: Measuring Mass and Plasmonic Signaturesmentioning

confidence: 99%

Detection of cancer biomarkers in serum using a hybrid mechanical and optoplasmonic nanosensor

et al. 2014

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Blood contains a range of protein biomarkers that could be used in the early detection of disease. To achieve this, however, requires sensors capable of detecting (with high reproducibility) biomarkers at concentrations one million times lower than the concentration of the other blood proteins. Here, we show that a sandwich assay that combines mechanical and optoplasmonic transduction can detect cancer biomarkers in serum at ultralow concentrations. A biomarker is first recognized by a surface-anchored antibody and then by an antibody in solution that identifies a free region of the captured biomarker. This second antibody is tethered to a gold nanoparticle that acts as a mass and plasmonic label; the two signatures are detected by means of a silicon cantilever that serves as a mechanical resonator for 'weighing' the mass of the captured nanoparticles and as an optical cavity that boosts the plasmonic signal from the nanoparticles. The capabilities of the approach are illustrated with two cancer biomarkers: the carcinoembryonic antigen and the prostate specific antigen, which are currently in clinical use for the diagnosis, monitoring and prognosis of colon and prostate cancer, respectively. A detection limit of 1 × 10(-16) g ml(-1) in serum is achieved with both biomarkers, which is at least seven orders of magnitude lower than that achieved in routine clinical practice. Moreover, the rate of false positives and false negatives at this concentration is extremely low, ∼10(-4).

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“…Because the photonic microchips are planar, the total cell volume consumed by the photonic chips is limited. Therefore, the photonic chips do not seem to have a strong influence on cell viability [34], although their diameter of a few micrometers is reasonably large when compared to typical mammalian cells.…”

Section: B Raman Spectroscopymentioning

confidence: 99%

Silicon and silicon nitride photonic circuits for spectroscopic sensing on-a-chip [Invited]

et al. 2015

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There is a rapidly growing demand to use silicon and silicon nitride (Si 3 N 4 ) integrated photonics for sensing applications, ranging from refractive index to spectroscopic sensing. By making use of advanced CMOS technology, complex miniaturized circuits can be easily realized on a large scale and at a low cost covering visible to mid-IR wavelengths. In this paper we present our recent work on the development of silicon and Si 3 N 4 -based photonic integrated circuits for various spectroscopic sensing applications. We report our findings on waveguide-based absorption, and Raman and surface enhanced Raman spectroscopy. Finally we report on-chip spectrometers and on-chip broadband light sources covering very near-IR to mid-IR wavelengths to realize fully integrated spectroscopic systems on a chip.

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Silicon chips detect intracellular pressure changes in living cells

Cited by 70 publications

References 29 publications

Measuring forces and stressesin situin living tissues

Measuring forces and stressesin situin living tissues

Detection of cancer biomarkers in serum using a hybrid mechanical and optoplasmonic nanosensor

Silicon and silicon nitride photonic circuits for spectroscopic sensing on-a-chip [Invited]

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