Detection of cancer biomarkers in serum using a hybrid mechanical and optoplasmonic nanosensor

Kosaka, Priscila M.; Pini, Valerio; Ruz, José J.; Silva, Rubens A.; González, María Ujué; Ramos, Daniel; Calleja, Montserrat; Tamayo, Javier

doi:10.1038/nnano.2014.250

Cited by 229 publications

(179 citation statements)

References 38 publications

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“…spectroscopy | sensing | SERS | slippery surfaces | nanoparticles U ltrasensitive detection of chemicals and biological species is important in a broad range of scientific and technological fields ranging from analytical chemistry, materials, and biomolecular diagnostics (1)(2)(3)(4)(5) to the inspection of pollutants, explosives, and pharmaceutical drugs (6)(7)(8). Among various analytical techniques, surface-enhanced Raman scattering (SERS) is among the most promising methods in detecting trace amounts of molecules owing to its high molecular specificity (i.e., differentiation between different types of molecules) and high sensitivity (i.e., the lowest analyte concentration from which SERS signals are distinguishable from the noise signal of a control sample) (9)(10)(11)(12)(13)(14)(15)(16)(17).…”

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confidence: 99%

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Yang

Dai

Stogin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

608

439

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Detecting target analytes with high specificity and sensitivity in any fluid is of fundamental importance to analytical science and technology. Surface-enhanced Raman scattering (SERS) has proven to be capable of detecting single molecules with high specificity, but achieving single-molecule sensitivity in any highly diluted solutions remains a challenge. Here we demonstrate a universal platform that allows for the enrichment and delivery of analytes into the SERSsensitive sites in both aqueous and nonaqueous fluids, and its subsequent quantitative detection of Rhodamine 6G (R6G) down to ∼75 fM level (10 −15 mol·L −1 ). Our platform, termed slippery liquidinfused porous surface-enhanced Raman scattering (SLIPSERS), is based on a slippery, omniphobic substrate that enables the complete concentration of analytes and SERS substrates (e.g., Au nanoparticles) within an evaporating liquid droplet. Combining our SLIPSERS platform with a SERS mapping technique, we have systematically quantified the probability, p(c), of detecting R6G molecules at concentrations c ranging from 750 fM (p > 90%) down to 75 aM (10 −18 mol·L −1 ) levels (p ≤ 1.4%). The ability to detect analytes down to attomolar level is the lowest limit of detection for any SERS-based detection reported thus far. We have shown that analytes present in liquid, solid, or air phases can be extracted using a suitable liquid solvent and subsequently detected through SLIPSERS. Based on this platform, we have further demonstrated ultrasensitive detection of chemical and biological molecules as well as environmental contaminants within a broad range of common fluids for potential applications related to analytical chemistry, molecular diagnostics, environmental monitoring, and national security.spectroscopy | sensing | SERS | slippery surfaces | nanoparticles

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mentioning

confidence: 99%

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Yang

Dai

Stogin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

608

439

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“…A micro-to nanoscale size sensor can detect mass changes from pg to fg, which means a large enhancement compared with the limits of detection of other kinds of sensors. Kosaka et al (31) realized the detection of cancer markers in serum using a dynamic mode and achieved a limit of detection of 1 × 10 −16 g/mL. However, owing to the influence of a damping phenomena, it is difficult for a microcantilever biosensor with a micro to nanoscale size to function stably in a liquid environment.…”

Section: Microcantilever Biosensormentioning

confidence: 99%

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

2018

Sensors and Materials

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There are a variety of methods to detect odorant molecules, including gas chromatography (GC) and its related coupled techniques, electronic noses, and olfactory receptor (OR)-based biosensors. Nevertheless, studies in which cell and cell signaling amplifier systems were directly utilizied to detect odors are very few. In this review, we aim to provide some references and suggestions for researchers in related fields by summarizing OR sensors and olfactory cell signaling cascade amplifier systems. In this paper, we summarize the detection methods for odorants, introduce the research and progress on OR sensors, and present a proposal of utilizing cell or tissue signaling cascade amplifier systems to amplify electrochemical signals and the use of the G protein signaling cascade amplifier system to prepare electrochemical biosensors. In recent years, the detection technology of electrochemical sensors has matured. Meanwhile, olfactory sensation within organisms basically transfer nerve signals or metabolic endocrine signals in the form of electrochemical signals. Therefore, ORs combined with cell signaling cascade amplifier systems, as well as electrochemical sensors of signal cascade amplifier systems, have incredible prospects for development and application. The proposal of OR electrochemical biosensors and their cell signaling cascade amplifier systems, provides a new idea and a quantitative method for the detection and assessment of odorants and their physiologic processes in the nervous system.

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“…Kosaka e colaborabores 107 conseguiram combinar propriedades optoplasmônicas e mecânicas em um transdutor LSPR para a detecção de marcadores de câncer. Nesse trabalho o biomarcador é primeiramente reconhecido por um anticorpo ancorado à superfície e, em seguida, um segundo anticorpo em solução identifica a região livre do biomarcador ancorado.…”

Section: Figura 6 (A) Sensibilidade a Mudanças No íNdice De Refraçãounclassified

Ressonância de plasmon de superfície localizado e aplicação em biossensores e células solares

Santos¹,

Santos²,

Thesing³

et al. 2016

Quim. Nova

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LOCALIZED SURFACE PLASMON RESSONANCE APPLIED TO BIOSENSORS AND SOLAR CELLS. Within the last decades, the research on nanoparticles presenting localized surface plasmon resonance has increased constantly. In these materials, the interaction between electrons and incident light results in charge separation, enhancement of the electromagnetic field on the nanoparticles surface and in unique optical properties. Although many metals such as gold, silver, copper and aluminum present localized surface plasmon resonance within the visible range, gold and silver are the most commonly studied metals, due to the chemical inertia of gold and intense plasmon resonance from silver. In this review, we provide a description of the origin of localized surface plasmon resonance through the works developed by Mie, Maxwell and Maxwell-Garnett and a description of many examples of application of plasmonic nanoparticles on biosensors and solar cells, detailing the contribution of these plasmonic nanoparticles on the performance of these devices.Keywords: LSPR; Localized Surface Plasmon Resonance; biosensor; solar cell. INTRODUÇÃONos últimos 20 anos o interesse por nanopartículas metálicas (NPs-M) tem aumentado constantemente devido a sua ampla variedade de aplicações.1-8 Vários grupos de pesquisa têm estudado desde nanopartículas isoladas de cerca de 1 nm até nanopartículas de centenas de nanômetros, assim como seus aglomerados, buscando entender seu comportamento óptico e catalítico. Adicionalmente, a literatura descreve a constante busca por metodologias de síntese cada vez mais simples, de baixo custo e com alta reprodutibilidade. O comportamento óptico de nanopartículas metálicas tem fascinado a humanidade há vários séculos através de obras de arte, mesmo antes que a palavra nanotecnologia fosse utilizada.9 Um dos exemplos mais conhecidos é a Taça de Lycurgus (século 4 d.C), que tem em sua composição nanopartículas de ouro (NPAu).10 No entanto, foi somente em 1857 que as NPs-M receberam maior atenção devido aos estudos de Michael Faraday sobre o efeito da interação da luz em uma solução coloidal de NPAu obtida através da redução do cloreto de ouro pelo fósforo.11,12 Deste então, o interesse nestas NPs tem aumentado e novas metodologias de síntese e caracterização têm sido desenvolvidas, possibilitando grande controle de tamanho e formato.13-15 A pesquisa nesta área foi enriquecida pela compreensão do efeito da ressonância de plasmon de superfície localizado (LSPR), que explica o comportamento óptico destes materiais. Mie foi o primeiro a elucidar a origem das cores nestas estruturas e sua teoria foi posteriormente complementada com os modelos de Maxwell-Garnett que explicavam a interação de NPs-M com o meio. Estes estudos proporcionaram uma ampla visão quanto as possíveis aplicações das NPs-M, sendo atualmente empregada no desenvolvimento de novos materiais como células solares, biossensores e catalisadores. Nesta revisão, serão abordados os embasamentos teóricos envolvidos no efeito LSPR de NPs-M, destacando a aplicação destas n...

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Detection of cancer biomarkers in serum using a hybrid mechanical and optoplasmonic nanosensor

Cited by 229 publications

References 38 publications

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Ultrasensitive surface-enhanced Raman scattering detection in common fluids

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

Ressonância de plasmon de superfície localizado e aplicação em biossensores e células solares

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