Silica bead-based microfluidic device with integrated photodiodes for the rapid capture and detection of rolling circle amplification products in the femtomolar range

Soares, Ruben R. G.; Neumann, Felix; Caneira, Catarina R.F.; Madaboosi, Narayanan; Ciftci, Sibel; Hernández-Neuta, Iván; Pinto, Inês F.; Santos, Denis R.; Chu, V.; Russom, Aman; Conde, J.P.; Nilsson, Mats

doi:10.1016/j.bios.2018.12.004

Cited by 33 publications

(18 citation statements)

References 34 publications

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“…Silicon bead-based microfluidics can detect the amplification product in the femtomolar range. 64 Nanopores fabricated in the silicon nitride membrane can quantify DNA concentration in attomoles. 65 A label-free impedimetric electrochemical biosensor identifies Zika DNA in a nanomolar range.…”

Section: Diagnostic Approaches To Covid-19mentioning

confidence: 99%

Current and Perspective Diagnostic Techniques for COVID-19

et al. 2020

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Since late December 2019, the coronavirus pandemic (COVID-19; previously known as 2019-nCoV) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been surging rapidly around the world. With more than 1,700,000 confirmed cases, the world faces an unprecedented economic, social, and health impact. The early, rapid, sensitive, and accurate diagnosis of viral infection provides rapid responses for public health surveillance, prevention, and control of contagious diffusion. More than 30% of the confirmed cases are asymptomatic, and the high false-negative rate (FNR) of a single assay requires the development of novel diagnostic techniques, combinative approaches, sampling from different locations, and consecutive detection. The recurrence of discharged patients indicates the need for long-term monitoring and tracking. Diagnostic and therapeutic methods are evolving with a deeper understanding of virus pathology and the potential for relapse. In this Review, a comprehensive summary and comparison of different SARS-CoV-2 diagnostic methods are provided for researchers and clinicians to develop appropriate strategies for the timely and effective detection of SARS-CoV-2. The survey of current biosensors and diagnostic devices for viral nucleic acids, proteins, and particles and chest tomography will provide insight into the development of novel perspective techniques for the diagnosis of COVID-19.

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Section: Diagnostic Approaches To Covid-19mentioning

confidence: 99%

Current and Perspective Diagnostic Techniques for COVID-19

et al. 2020

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“…When mixed with microfluidic systems, some benefits like rapidity and cheapness are present. Rolling circle amplification combined with on‐chip size‐selective trapping of amplicons on silica beads showed that this system could be applied to diagnosing Ebola and influenza viruses 44 . In another study, Ciftci et al showed that traditional approaches like virus isolation, serology, immunoassays, and RT‐PCR are difficult and limited in terms of specificity and sensitivity for detecting RNA viruses.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

“…As a result, these devices may also help detect SARS‐CoV‐2 and accelerate the process of diagnosis and rehabilitation, ultimately lowering the death rate. These devices can use several techniques such as RT‐PCR, RT‐LAMP, nested PCR, nucleic acid hybridization, ELISA, or fluorescence‐based Assays 40‐44 …”

Section: Introductionmentioning

confidence: 99%

Microfluidic devices for detection of RNA viruses

Basiri

Heidari

Nadi

et al. 2020

Reviews in Medical Virology

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Summary There is a long way to go before the coronavirus disease 2019 (Covid‐19) outbreak comes under control. qRT‐PCR is currently used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), the causative agent of Covid‐19, but it is expensive, time‐consuming, and not as sensitive as it should be. Finding a rapid, easy‐to‐use, and cheap diagnostic method is necessary to help control the current outbreak. Microfluidic systems provide a platform for many diagnostic tests, including RT‐PCR, RT‐LAMP, nested‐PCR, nucleic acid hybridization, ELISA, fluorescence‐Based Assays, rolling circle amplification, aptamers, sample preparation multiplexer (SPM), Porous Silicon Nanowire Forest, silica sol‐gel coating/bonding, and CRISPR. They promise faster, cheaper, and easy‐to‐use methods with higher sensitivity, so microfluidic devices have a high potential to be an alternative method for the detection of viral RNA. These devices have previously been used to detect RNA viruses such as H1N1, Zika, HAV, HIV, and norovirus, with acceptable results. This paper provides an overview of microfluidic systems as diagnostic methods for RNA viruses with a focus on SARS‐CoV‐2.

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“…In many cases, the concept of the polymerase chain reaction is also useful to amplify the numbers of copies of specific genetic samples, such as RNA samples from Influenza [149,150]. Moreover, a point-of-care device with a self-powered system is a promising innovation to provide the potential in personalized diagnostic devices.…”

Section: Moving Toward Modern Electrochemical Detection Of Virusesmentioning

confidence: 99%

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

2020

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Personal biosensors and bioelectronics have been demonstrated for use in out-of-clinic biomedical devices. Such modern devices have the potential to transform traditional clinical analysis into a new approach, allowing patients or users to screen their own health or warning of diseases. Researchers aim to explore the opportunities of easy-to-wear and easy-to-carry sensors that would empower users to detect biomarkers, electrolytes, or pathogens at home in a rapid and easy way. This mobility would open the door for early diagnosis and personalized healthcare management to a wide audience. In this review, we focus on the recent progress made in modern electrochemical sensors, which holds promising potential to support point-of-care technologies. Key original research articles covered in this review are mainly experimental reports published from 2018 to 2020. Strategies for the detection of metabolites, ions, and viruses are updated in this article. The relevant challenges and opportunities of applying nanomaterials to support the fabrication of new electrochemical biosensors are also discussed. Finally, perspectives regarding potential benefits and current challenges of the technology are included. The growing area of personal biosensors is expected to push their application closer to a new phase of biomedical advancement.

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Silica bead-based microfluidic device with integrated photodiodes for the rapid capture and detection of rolling circle amplification products in the femtomolar range

Cited by 33 publications

References 34 publications

Current and Perspective Diagnostic Techniques for COVID-19

Current and Perspective Diagnostic Techniques for COVID-19

Microfluidic devices for detection of RNA viruses

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

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