Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen)

Unique synteny and alternate splicing of the chitin synthases in closely related heliothine moths

“…An alternative splicing product was observed in 2 nd and 3 rd instar larvae and muscle of 4 th and 5 th instar larvae. Similar alternative splicing products have been reported in T. castaneum, M. sexta, A. gambiae, D. melanogaster, P. xylostella, S. exigua, O. furnacalis and L. migratoria manilensis(Gagou et al 2002;Arakane et al 2004;Hogenkamp et al 2005;Ashfaq et al 2007;Chen et al 2007;Zhang et al 2010;Zhang et al 2012) where the splice variants differ in a 59 amino acid insertion. The major tissues for HzChSB expression were in the foregut, midgut and hindgut (Fig.…”

“…Bactrocera dorsalis (Yang et al 2013); the moths Manduca sexta (Zhu et al 2002;Hogenkamp et al 2005), Ostrinia furnacalis Qu and Yang 2012), Plutella xylostella (Ashfaq et al 2007), Spodoptera exigua (Chen et al 2007; Kumar et al 2008), S. frugiperda (Bolognesi et al 2005); the beetle, Tribolium castaneum (Arakane et al 2004); the locust, Locusta migratoria manilensis (Zhang et al 2010); and the hemipterans, Aphis glycines (Bansal et al 2012), Laodelphax striatellus (Wang et al 2012) and Nilaparvata lugens (Wang et al 2012). However, in these three hemipterans only the ChSA were present while genes for ChSB were absent, which is consistent with the lack of PM in these hemipterans (Bansal et al 2012;Wang et al 2012).…”

“…-7 -helix Rezende et al 2008;Zhang et al 2010;Qu and Yang 2012). These variants are differentially expressed in the epidermis and tracheae during insect development (Arakane et al 2004;Hogenkamp et al 2005;Zimoch et al 2005;).…”

Two chitinase 5 genes from Locusta migratoria: Molecular characteristics and functional differentiation

“…For the high expression level of LmCht5-1 in adults, we hypothesized that LmCht5-1 might be involved in early adult development or fecundity of locusts. Indeed high transcripts of chitin synthase 1 have been found in L. migratoria (Zhang et al, 2010) and T. castaneum (Arakane et al, 2008) during the early adult stage. These results suggest that chitin metabolic pathways could be still very active in the early adult stage.…”

RNA interference: Applications and advances in insect toxicology and insect pest management

“…Apparently, different levels of RNAi-mediated suppression of a target gene can significantly affect the outcome. Even for the same target gene, a low level of suppression of its transcript in one study or in an insect with low RNAi efficiency may not display a lethal effect, but a high level of suppression in other study or in an insect with robust RNAi response may result in a strong lethal effect [79,80]. Although it has been less well documented, different dsRNA delivery methods for the same target gene may also lead to different outcomes due to possible differences in cellular uptake and degradation of dsRNA, or due to differential expression of genes involved in the RNAi pathway in different tissues of an insect species.…”