Sika Deer Antler Collagen Type I‐Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway

Li, Na; Zhang, Min; Drummen, Gregor P. C.; Zhao, Yu; Tan, Yin Fen; Luo, Shihua; Qu, Xiao Bo

doi:10.1155/2016/2109204

Cited by 6 publications

(10 citation statements)

References 56 publications

(59 reference statements)

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“…that of TCP, most probably through conventional signalling mechanisms. Consistent with this conclusion, it was reported that the interaction of cell surface integrins with collagenous compounds of scaffolds activated signalling pathways inducing osteogenic specific genes expression [44,45].…”

Section: Discussionsupporting

confidence: 68%

Osteogenic differentiation of Wharton’s jelly-derived mesenchymal stem cells cultured on WJ-scaffold through conventional signalling mechanism

Beiki

Zeynali

Taghiabadi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Wharton's jelly-derived extracellular matrix (WJ-ECM) has attracted researcher's attention for its biomedical applications. Previously, we fabricated a biomimetic spongy scaffold from decellularized WJ-ECM and, in this study, we sought to examine the osteogenic inductive potential of this scaffold and its underlying mechanism. To address this question, mesenchymal stem cells (MSCs) were isolated from WJ using a mechanical method and cultured on the scaffold, under dynamic condition, for over 21 days in the presence or absence of osteogenic medium. The status of signalling pathways involved in the osteogenic differentiation and the expression profile of integrins in the WJ-derived MSCs (WJ-MSCs) were examined. WJ-MSCs displayed differentiation capacities and expressed surface antigens, characteristics of MSCs. Histologically, WJ-MSCs seeded on the scaffold showed a proper cellular attachment, penetration and migration. They also exhibited a higher degree of alkaline phosphatase activity, calcium deposition and osteogenic gene expression, than those cultured in 2D condition. The expression of Wnt, BMP and TGF-b signalling target genes together with that of a2, av and b1 integrins was increased in WJ-MSCs in both presence and absence of osteogenic induction medium. Taken together, our results demonstrate that WJ-derived scaffold induces osteogenic differentiation of WJ-MSCs, possibly through activating integrins and subsequently conventional intracellular signalling pathways.

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Section: Discussionsupporting

confidence: 68%

Osteogenic differentiation of Wharton’s jelly-derived mesenchymal stem cells cultured on WJ-scaffold through conventional signalling mechanism

Beiki

Zeynali

Taghiabadi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…Rat BMSCs were isolated and confirmed using a modification of methods previously described (Na et al. 2016 ) and put in the freezer (−80 °C) for further use. The study was made to comply with the Animal Research: Reporting In Vivo Experiments (ARRIVE) guidelines (Changchun University of Chinese Medicine ethical approval number: Protocol Number 2014-08-15).…”

Section: Methodsmentioning

confidence: 99%

“…SPC-I was extracted by a trypsin hydrolysis method as described as previously (Na et al. 2016 ), and protein concentration was 56.89%. To exclude the other protein peptide residues, high performance liquid chromatography (Agilent 1100 series; Agilent Technologies, Lexington, MA) was used to purify the SPC-I.…”

Section: Methodsmentioning

confidence: 99%

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Sika pilose antler type I collagen promotes BMSC differentiation via the ERK1/2 and p38-MAPK signal pathways

Wang

Luo

Zhang

et al. 2017

Pharmaceutical Biology

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Context: Sika pilose antler type I collagen (SPC-I) have been reported to promote bone marrow mesenchymal stem cell (BMSC) proliferation and differentiation. However, the underlying mechanism is still unclear.Objective: This study investigates the molecular mechanisms of SPC-I on the BMSC proliferation and differentiation of osteoblast (OB) in vitro.Material and methods: The primary rat BMSC was cultured and exposed to SPC-I at different concentrations (2.5, 5.0 and 10.0 mg/mL) for 20 days. The effect of SPC-I on the differentiation of BMSCs was evaluated through detecting the activity of alkaline phosphatase (ALP), ALP staining, collagen I (Col-I) content, and calcified nodules. The markers of osteoblastic differentiation were evaluated using RT-PCR and Western-blot analysis.Results: SPC-I treatment (2.5 mg/mL) significantly increased the proliferation of BMSCs (p < 0.01), whereas, SPC-I (5.0 and 10.0 mg/mL) significantly inhibited the proliferation of BMSCs (p < 0.01). SPC-I (2.5 mg/mL) significantly increased ALP activity and Col-I content (p < 0.01), and increased positive cells in ALP staining and the formation of calcified nodules. Additionally, the gene expression of ALP, Col-I, Osteocalcin (OC), Runx2, Osterix (Osx), ERK1/2, BMP2 and p38-MAPK, along with the protein expression of ERK1/2, p-ERK1/2, p-p38 MAPK were markedly increased in the SPC-I (5.0 mg/mL) treatment group (p < 0.01) compared to the control group.Discussion and conclusions: SPC-I can induce BMSC differentiation into OBs and enhance the function of osteogenesis through ERK1/2 and p38-MAPK signal transduction pathways and regulating the gene expression of osteogenesis-specific transcription factors.

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“…This osteoclast precursor and its membrane surface can express the receptor activator of the nf-kappa B (RANK) [18,19]. Under physiological conditions, RANKL comes into contact with RANK, promoting osteoclast differentiation and function.…”

Section: Anti-osteoporotic Activities Of the Collagen Extracted From mentioning

confidence: 99%

Anti-osteoporotic activities of the collagen extracted from Carapacis et Plastri Testudinis on a ROS1728 cell line in vitro

Wang¹,

Wu²,

Jiang³

et al. 2018

biomedicalresearch

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Sika Deer Antler Collagen Type I‐Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway

Cited by 6 publications

References 56 publications

Osteogenic differentiation of Wharton’s jelly-derived mesenchymal stem cells cultured on WJ-scaffold through conventional signalling mechanism

Osteogenic differentiation of Wharton’s jelly-derived mesenchymal stem cells cultured on WJ-scaffold through conventional signalling mechanism

Sika pilose antler type I collagen promotes BMSC differentiation via the ERK1/2 and p38-MAPK signal pathways

Anti-osteoporotic activities of the collagen extracted from Carapacis et Plastri Testudinis on a ROS1728 cell line in vitro

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