Sika pilose antler type I collagen promotes BMSC differentiation via the ERK1/2 and p38-MAPK signal pathways

Abstract

Context: Sika pilose antler type I collagen (SPC-I) have been reported to promote bone marrow mesenchymal stem cell (BMSC) proliferation and differentiation. However, the underlying mechanism is still unclear.Objective: This study investigates the molecular mechanisms of SPC-I on the BMSC proliferation and differentiation of osteoblast (OB) in vitro.Material and methods: The primary rat BMSC was cultured and exposed to SPC-I at different concentrations (2.5, 5.0 and 10.0 mg/mL) for 20 days. The effect of SPC-I on the differentiation of BMSCs was evaluated through detecting the activity of alkaline phosphatase (ALP), ALP staining, collagen I (Col-I) content, and calcified nodules. The markers of osteoblastic differentiation were evaluated using RT-PCR and Western-blot analysis.Results: SPC-I treatment (2.5 mg/mL) significantly increased the proliferation of BMSCs (p < 0.01), whereas, SPC-I (5.0 and 10.0 mg/mL) significantly inhibited the proliferation of BMSCs (p < 0.01). SPC-I (2.5 mg/mL) significantly increased ALP activity and Col-I content (p < 0.01), and increased positive cells in ALP staining and the formation of calcified nodules. Additionally, the gene expression of ALP, Col-I, Osteocalcin (OC), Runx2, Osterix (Osx), ERK1/2, BMP2 and p38-MAPK, along with the protein expression of ERK1/2, p-ERK1/2, p-p38 MAPK were markedly increased in the SPC-I (5.0 mg/mL) treatment group (p < 0.01) compared to the control group.Discussion and conclusions: SPC-I can induce BMSC differentiation into OBs and enhance the function of osteogenesis through ERK1/2 and p38-MAPK signal transduction pathways and regulating the gene expression of osteogenesis-specific transcription factors.

Calycosin stimulates the osteogenic differentiation of rat calvarial osteoblasts by activating the IGF1R/PI3K/Akt signaling pathway

“…Its formation and expression can control and stimulate osteoblast adhesion and differentiation (Dawson‐Hughes et al, ; Hu et al, ). Runt‐related transcription factor 2 ( Runx2 ) and Osterix are two essential transcription factors in the early and late stages of osteoblast differentiation and bone formation; they affect the expression of genes responsible for the production of bone specific matrix proteins, including ALP, COL1, BSP, and OCN, and eventually promote cell matrix mineralization (Nakashima et al, ; Hinoi et al, ; Ying et al, ; Wang et al, ). The bone morphogenetic protein (BMP) family, with more than 20 members, consists of multifunctional extracellular growth factors in the transforming growth factor‐β superfamily which play a pivotal role in numerous biological processes in the developing embryo and in the adult.…”

Expression of microRNA‑21 in osteoporotic patients and its involvement in the regulation of osteogenic differentiation

“…Under certain induction conditions, they can differentiate into many kinds of cells, such as osteoblasts, adipocytes, chondrocytes, cardiomyocytes and nerve cells (18). The osteogenic potential of BMSCs plays an important role in the treatment of OP, bone fractures, bone necrosis, osteoarthritis and bone defects (19,20). Therefore, to study the differentiation of BMSCs is of great significance for the treatment of OP.…”

Effect of CD44 on differentiation of human amniotic mesenchymal stem cells into chondrocytes via Smad and ERK signaling pathways

“…a previous study reported that the MaPK signaling pathway, including erK1/2, JnK and p38 MaPK molecules, is activated in chondrocytes (19). activation of erK signaling pathway can enhance the ability of MScs to differentiate into osteoblasts and cardiomyocytes (20,21). in addition, the erK1/2 signaling pathway regulates chondrocyte differentiation and cartilage formation in in vitro (22).…”